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Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization

A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enz...

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Main Authors: Mailin Misson, Bo, Jin, Binghui, Chen, Hu, Zhang
Format: Article
Language:English
English
Published: Springer Berlin Heidelberg 2015
Subjects:
TA Engineering (General). Civil engineering (General)
Online Access:https://eprints.ums.edu.my/id/eprint/19596/1/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2.pdf
https://eprints.ums.edu.my/id/eprint/19596/7/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2-galactosidase%20through%20functionalized%20polymer%20nanofiber%20immobilization.pdf
https://eprints.ums.edu.my/id/eprint/19596/
https://doi.org/10.1007/s00449-015-1432-5
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spelling my.ums.eprints.195962020-12-09T02:50:13Z https://eprints.ums.edu.my/id/eprint/19596/ Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization Mailin Misson Bo, Jin Binghui, Chen Hu, Zhang TA Engineering (General). Civil engineering (General) A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enzyme binding. The bioengineering performance of the enzyme carriers was further evaluated for bioconversion of lactose to galacto-oligosaccharides (GOS). The modified PSNF-Gal demonstrated distinguished performances to preserve the same activity as the free β-galactosidase at the optimum pH of 7.0, and to enhance the enzyme stability of PSNF-Gal in an alkaline condition up to pH 10. The PSNF assembly demonstrated improved thermal stability from 37 to 60 °C. The nanobiocatalyst was able to retain 30 % of its initial activity after ninth operation cycles comparing to four cycles with the unmodified counterpart. In contrast with free β-galactosidase, the modified PSNF-Gal enhanced the GOS yield from 14 to 28 %. These findings show the chemically modified PSNF-based nanobiocatalyst may be pertinent for various enzyme-catalysed bioprocessing applications. Springer Berlin Heidelberg 2015-10 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19596/1/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2.pdf text en https://eprints.ums.edu.my/id/eprint/19596/7/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2-galactosidase%20through%20functionalized%20polymer%20nanofiber%20immobilization.pdf Mailin Misson and Bo, Jin and Binghui, Chen and Hu, Zhang (2015) Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization. Bioprocess and Biosystems Engineering, 38 (10). pp. 1915-1923. ISSN 1615-7605 https://doi.org/10.1007/s00449-015-1432-5
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
English
topic TA Engineering (General). Civil engineering (General)
spellingShingle TA Engineering (General). Civil engineering (General)
Mailin Misson
Bo, Jin
Binghui, Chen
Hu, Zhang
Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
description A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enzyme binding. The bioengineering performance of the enzyme carriers was further evaluated for bioconversion of lactose to galacto-oligosaccharides (GOS). The modified PSNF-Gal demonstrated distinguished performances to preserve the same activity as the free β-galactosidase at the optimum pH of 7.0, and to enhance the enzyme stability of PSNF-Gal in an alkaline condition up to pH 10. The PSNF assembly demonstrated improved thermal stability from 37 to 60 °C. The nanobiocatalyst was able to retain 30 % of its initial activity after ninth operation cycles comparing to four cycles with the unmodified counterpart. In contrast with free β-galactosidase, the modified PSNF-Gal enhanced the GOS yield from 14 to 28 %. These findings show the chemically modified PSNF-based nanobiocatalyst may be pertinent for various enzyme-catalysed bioprocessing applications.
format Article
author Mailin Misson
Bo, Jin
Binghui, Chen
Hu, Zhang
author_facet Mailin Misson
Bo, Jin
Binghui, Chen
Hu, Zhang
author_sort Mailin Misson
title Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
title_short Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
title_full Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
title_fullStr Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
title_full_unstemmed Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
title_sort enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
publisher Springer Berlin Heidelberg
publishDate 2015
url https://eprints.ums.edu.my/id/eprint/19596/1/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2.pdf
https://eprints.ums.edu.my/id/eprint/19596/7/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2-galactosidase%20through%20functionalized%20polymer%20nanofiber%20immobilization.pdf
https://eprints.ums.edu.my/id/eprint/19596/
https://doi.org/10.1007/s00449-015-1432-5
_version_ 1760229602218737664
score 13.211869

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