Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enz...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Springer Berlin Heidelberg
2015
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/19596/1/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2.pdf https://eprints.ums.edu.my/id/eprint/19596/7/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2-galactosidase%20through%20functionalized%20polymer%20nanofiber%20immobilization.pdf https://eprints.ums.edu.my/id/eprint/19596/ https://doi.org/10.1007/s00449-015-1432-5 |
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| Summary: | A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enzyme binding. The bioengineering performance of the enzyme carriers was further evaluated for bioconversion of lactose to galacto-oligosaccharides (GOS). The modified PSNF-Gal demonstrated distinguished performances to preserve the same activity as the free β-galactosidase at the optimum pH of 7.0, and to enhance the enzyme stability of PSNF-Gal in an alkaline condition up to pH 10. The PSNF assembly demonstrated improved thermal stability from 37 to 60 °C. The nanobiocatalyst was able to retain 30 % of its initial activity after ninth operation cycles comparing to four cycles with the unmodified counterpart. In contrast with free β-galactosidase, the modified PSNF-Gal enhanced the GOS yield from 14 to 28 %. These findings show the chemically modified PSNF-based nanobiocatalyst may be pertinent for various enzyme-catalysed bioprocessing applications. |
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