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Determination of nicotine content in various tobacco products using capillary electrophoresis

This study was aim to optimize the separation of nicotine in tobacco products sample. The tobacco products are cigarette and local chewing tobacco. The main objective of the study is to optimize capillary electrophoresis (CE) separation for nicotine. Two type of buffer was compared Citrate buffer a...

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Main Author: Romero Robby David Ubing
Format: Academic Exercise
Language:English
Published: 2010
Subjects:
TP Chemical technology
Online Access:https://eprints.ums.edu.my/id/eprint/11725/1/ae0000002687.pdf
https://eprints.ums.edu.my/id/eprint/11725/
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spelling my.ums.eprints.117252017-10-27T02:16:04Z https://eprints.ums.edu.my/id/eprint/11725/ Determination of nicotine content in various tobacco products using capillary electrophoresis Romero Robby David Ubing TP Chemical technology This study was aim to optimize the separation of nicotine in tobacco products sample. The tobacco products are cigarette and local chewing tobacco. The main objective of the study is to optimize capillary electrophoresis (CE) separation for nicotine. Two type of buffer was compared Citrate buffer and sodium phosphate, where both buffer was also tested at different pH pH 2.5, 4.5 and 9.5 .Sodium phosphate buffer with pH 2.5 was chosen as a running buffer due to high and symmetrical peak. The buffer then is tested on using different concentration 10mM, 25mM, 50mM and 100mM. Buffer with a high concentration increases the migration time slightly. Based .on the result sodium phosphate buffer pH 2.5 with 100mM was use in the sample analyze using CE and the condition was 10cm capillary length, UV detection at 260 nm, sample injection 0.5 psi, for 5 s at 30ºC with 5 kV applied voltage. Sample was analyze using the optimized method after extraction and the result was Cigarette (12.921 ppm) have almost double the nicotine contain compare to local chewing tobacco (7.572 ppm). 2010 Academic Exercise NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/11725/1/ae0000002687.pdf Romero Robby David Ubing (2010) Determination of nicotine content in various tobacco products using capillary electrophoresis. Universiti Malaysia Sabah. (Unpublished)
institution Universiti Malaysia Sabah
building UMS Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Sabah
content_source UMS Institutional Repository
url_provider http://eprints.ums.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Romero Robby David Ubing
Determination of nicotine content in various tobacco products using capillary electrophoresis
description This study was aim to optimize the separation of nicotine in tobacco products sample. The tobacco products are cigarette and local chewing tobacco. The main objective of the study is to optimize capillary electrophoresis (CE) separation for nicotine. Two type of buffer was compared Citrate buffer and sodium phosphate, where both buffer was also tested at different pH pH 2.5, 4.5 and 9.5 .Sodium phosphate buffer with pH 2.5 was chosen as a running buffer due to high and symmetrical peak. The buffer then is tested on using different concentration 10mM, 25mM, 50mM and 100mM. Buffer with a high concentration increases the migration time slightly. Based .on the result sodium phosphate buffer pH 2.5 with 100mM was use in the sample analyze using CE and the condition was 10cm capillary length, UV detection at 260 nm, sample injection 0.5 psi, for 5 s at 30ºC with 5 kV applied voltage. Sample was analyze using the optimized method after extraction and the result was Cigarette (12.921 ppm) have almost double the nicotine contain compare to local chewing tobacco (7.572 ppm).
format Academic Exercise
author Romero Robby David Ubing
author_facet Romero Robby David Ubing
author_sort Romero Robby David Ubing
title Determination of nicotine content in various tobacco products using capillary electrophoresis
title_short Determination of nicotine content in various tobacco products using capillary electrophoresis
title_full Determination of nicotine content in various tobacco products using capillary electrophoresis
title_fullStr Determination of nicotine content in various tobacco products using capillary electrophoresis
title_full_unstemmed Determination of nicotine content in various tobacco products using capillary electrophoresis
title_sort determination of nicotine content in various tobacco products using capillary electrophoresis
publishDate 2010
url https://eprints.ums.edu.my/id/eprint/11725/1/ae0000002687.pdf
https://eprints.ums.edu.my/id/eprint/11725/
_version_ 1760229230224867328
score 13.211869

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