Determination of nicotine content in various tobacco products using capillary electrophoresis
This study was aim to optimize the separation of nicotine in tobacco products sample. The tobacco products are cigarette and local chewing tobacco. The main objective of the study is to optimize capillary electrophoresis (CE) separation for nicotine. Two type of buffer was compared Citrate buffer a...
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| Main Author: | |
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| Format: | Academic Exercise |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/11725/1/ae0000002687.pdf https://eprints.ums.edu.my/id/eprint/11725/ |
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| Summary: | This study was aim to optimize the separation of nicotine in tobacco products sample. The tobacco products are cigarette and local chewing tobacco. The main objective of the
study is to optimize capillary electrophoresis (CE) separation for nicotine. Two type of buffer was compared Citrate buffer and sodium phosphate, where both buffer was also tested at different pH pH 2.5, 4.5 and 9.5 .Sodium phosphate buffer with pH 2.5 was chosen as a running buffer due to high and symmetrical peak. The buffer then is tested
on using different concentration 10mM, 25mM, 50mM and 100mM. Buffer with a high concentration increases the migration time slightly. Based .on the result sodium phosphate buffer pH 2.5 with 100mM was use in the sample analyze using CE and the condition was 10cm capillary length, UV detection at 260 nm, sample injection 0.5 psi, for 5 s at 30ºC with 5 kV applied voltage. Sample was analyze using the optimized
method after extraction and the result was Cigarette (12.921 ppm) have almost double the nicotine contain compare to local chewing tobacco (7.572 ppm). |
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