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Optimisation of Bioethanol Yield from Oil Palm Trunk Sap

This paper presents the effect of nutrients addition and fermentation microorganism on bioethanol yield from oil palm trunk sap. Six microorganisms namely, Saccharomyces cerevisiae ATCC 9763, Saccharomyces cerevisiae ATCC 26602, baker’s yeast, Kluyveromyces marxianus ATCC 46537, Zymomonas mobilis AT...

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Main Authors: Rossyuhaida, Mohd Zakria, Chua, Gek Kee, Jolius, Gimbun, Nor Shahirah, Mohd Nasir, Sook, Fun Pang, Mohd Fazli, Farida Asras, Cheng, C. K., Ahmad Ziad, Sulaiman
Format: Book Section
Language:English
Published: Universiti Teknikal Malaysia Melaka (UTeM) 2015
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/7607/1/Optimisation_of_Bioethanol_Yield_from_Oil_Palm_Trunk_Sap.pdf
http://umpir.ump.edu.my/id/eprint/7607/
http://ocs.utem.edu.my/index.php/mucet2014/MUCET2014/paper/viewFile/126/70
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spelling my.ump.umpir.76072018-01-10T07:43:59Z http://umpir.ump.edu.my/id/eprint/7607/ Optimisation of Bioethanol Yield from Oil Palm Trunk Sap Rossyuhaida, Mohd Zakria Chua, Gek Kee Jolius, Gimbun Nor Shahirah, Mohd Nasir Sook, Fun Pang Mohd Fazli, Farida Asras Cheng, C. K. Ahmad Ziad, Sulaiman TP Chemical technology This paper presents the effect of nutrients addition and fermentation microorganism on bioethanol yield from oil palm trunk sap. Six microorganisms namely, Saccharomyces cerevisiae ATCC 9763, Saccharomyces cerevisiae ATCC 26602, baker’s yeast, Kluyveromyces marxianus ATCC 46537, Zymomonas mobilis ATCC 29501 and Escherichia coli ATCC 10536 were screened for ethanol production at fixed temperature, pH, agitation and inoculum size. The sugar and ethanol content were determined using a high performance liquid chromatography (HPLC). The results showed that K. marxianus produced the highest ethanol yield (60.9%) at a shorter fermentation time (16h) compared to the other strains. Six nutrients, namely, ammonium sulphate, di-ammonium hydrogen phosphate, magnesium sulphate, β-alanine, calcium chloride and potassium dihydrogen phosphate were screened using this strain and the highest ethanol yield (98.62%) was achieved in fermentation supplemented by magnesium sulphate and β-alanine. Subsequently, the optimisation study using a reseponse surfae methodology found the optimum value of magnesium sulphate was 7.93 g/L and 0.90 g/L for β-alanine. Under the optimum conditions, the predicted ethanol concentration was 34.58 g/L while the experimental value (35.50 g/L) was in agreement with the predicted value with 2.66% error. Universiti Teknikal Malaysia Melaka (UTeM) 2015 Book Section NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7607/1/Optimisation_of_Bioethanol_Yield_from_Oil_Palm_Trunk_Sap.pdf Rossyuhaida, Mohd Zakria and Chua, Gek Kee and Jolius, Gimbun and Nor Shahirah, Mohd Nasir and Sook, Fun Pang and Mohd Fazli, Farida Asras and Cheng, C. K. and Ahmad Ziad, Sulaiman (2015) Optimisation of Bioethanol Yield from Oil Palm Trunk Sap. In: Proceeding Book Mucet 2014 : Engineering & Technology. Universiti Teknikal Malaysia Melaka (UTeM), Melaka, pp. 340-346. ISBN 978-967-0257-53-2 http://ocs.utem.edu.my/index.php/mucet2014/MUCET2014/paper/viewFile/126/70
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Rossyuhaida, Mohd Zakria
Chua, Gek Kee
Jolius, Gimbun
Nor Shahirah, Mohd Nasir
Sook, Fun Pang
Mohd Fazli, Farida Asras
Cheng, C. K.
Ahmad Ziad, Sulaiman
Optimisation of Bioethanol Yield from Oil Palm Trunk Sap
description This paper presents the effect of nutrients addition and fermentation microorganism on bioethanol yield from oil palm trunk sap. Six microorganisms namely, Saccharomyces cerevisiae ATCC 9763, Saccharomyces cerevisiae ATCC 26602, baker’s yeast, Kluyveromyces marxianus ATCC 46537, Zymomonas mobilis ATCC 29501 and Escherichia coli ATCC 10536 were screened for ethanol production at fixed temperature, pH, agitation and inoculum size. The sugar and ethanol content were determined using a high performance liquid chromatography (HPLC). The results showed that K. marxianus produced the highest ethanol yield (60.9%) at a shorter fermentation time (16h) compared to the other strains. Six nutrients, namely, ammonium sulphate, di-ammonium hydrogen phosphate, magnesium sulphate, β-alanine, calcium chloride and potassium dihydrogen phosphate were screened using this strain and the highest ethanol yield (98.62%) was achieved in fermentation supplemented by magnesium sulphate and β-alanine. Subsequently, the optimisation study using a reseponse surfae methodology found the optimum value of magnesium sulphate was 7.93 g/L and 0.90 g/L for β-alanine. Under the optimum conditions, the predicted ethanol concentration was 34.58 g/L while the experimental value (35.50 g/L) was in agreement with the predicted value with 2.66% error.
format Book Section
author Rossyuhaida, Mohd Zakria
Chua, Gek Kee
Jolius, Gimbun
Nor Shahirah, Mohd Nasir
Sook, Fun Pang
Mohd Fazli, Farida Asras
Cheng, C. K.
Ahmad Ziad, Sulaiman
author_facet Rossyuhaida, Mohd Zakria
Chua, Gek Kee
Jolius, Gimbun
Nor Shahirah, Mohd Nasir
Sook, Fun Pang
Mohd Fazli, Farida Asras
Cheng, C. K.
Ahmad Ziad, Sulaiman
author_sort Rossyuhaida, Mohd Zakria
title Optimisation of Bioethanol Yield from Oil Palm Trunk Sap
title_short Optimisation of Bioethanol Yield from Oil Palm Trunk Sap
title_full Optimisation of Bioethanol Yield from Oil Palm Trunk Sap
title_fullStr Optimisation of Bioethanol Yield from Oil Palm Trunk Sap
title_full_unstemmed Optimisation of Bioethanol Yield from Oil Palm Trunk Sap
title_sort optimisation of bioethanol yield from oil palm trunk sap
publisher Universiti Teknikal Malaysia Melaka (UTeM)
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/7607/1/Optimisation_of_Bioethanol_Yield_from_Oil_Palm_Trunk_Sap.pdf
http://umpir.ump.edu.my/id/eprint/7607/
http://ocs.utem.edu.my/index.php/mucet2014/MUCET2014/paper/viewFile/126/70
_version_ 1643665659254538240
score 13.211869

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