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Total phenolic content and antioxidant activity of some Malaysian herbs

Herbs have drawn much attention of people worldwide, not only because of their economic value as food products, but also for their antioxidant compounds. Thus, this study was conducted to investigate the total phenolic content and antioxidant activities on three Malaysian herbs by using Soxhlet extr...

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Main Author: Norhayantie, A.Rahman
Format: Undergraduates Project Papers
Language:English
Published: 2013
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/7226/1/Total_phenolic_content_and_antioxidant_activity.pdf
http://umpir.ump.edu.my/id/eprint/7226/
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spelling my.ump.umpir.72262021-06-09T03:04:12Z http://umpir.ump.edu.my/id/eprint/7226/ Total phenolic content and antioxidant activity of some Malaysian herbs Norhayantie, A.Rahman QD Chemistry Herbs have drawn much attention of people worldwide, not only because of their economic value as food products, but also for their antioxidant compounds. Thus, this study was conducted to investigate the total phenolic content and antioxidant activities on three Malaysian herbs by using Soxhlet extraction method. The herbs were Eugenia polyantha (Serai kayu), Euodia redlevi (Tenggek burung) and Limnocharis flava (Sudu itik). The total phenolic content and antioxidant compounds were extracted using distilled water at three different extraction time (4, 8 and 12 hour). The extracts were then evaluated using Folin-Ciocalteu reagent for their total phenolic content and 2, 2-Diphenyl-1-picrylhydrazyl hydrate (DPPH) assay for their antioxidant compounds. Further analyzed was done using High Performance Liquid Chromatography (HPLC) to further verifying the ascorbic acid existence in the extracts. It was found L. flava showed the highest content of total phenolic compounds (15.01 mg gallic acid equivalent (GAE) per 1 g of sample) with the antioxidant capacity standing at 20.42 mg ascorbic acid equivalent (AAE) per 1 g of sample after 8 hour extraction. While, E. polyantha showed the highest value of antioxidant capacity (21.19 mg AAE/g) with total phenolic content of 9.99 mg GAE/g. In HPLC analysis, it was found that E. polyantha and L. flava after 8 hour extraction contained 0.7625 and 0.9745 mg AAE/g of ascorbic acid respectively. 2013 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7226/1/Total_phenolic_content_and_antioxidant_activity.pdf Norhayantie, A.Rahman (2013) Total phenolic content and antioxidant activity of some Malaysian herbs. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Norhayantie, A.Rahman
Total phenolic content and antioxidant activity of some Malaysian herbs
description Herbs have drawn much attention of people worldwide, not only because of their economic value as food products, but also for their antioxidant compounds. Thus, this study was conducted to investigate the total phenolic content and antioxidant activities on three Malaysian herbs by using Soxhlet extraction method. The herbs were Eugenia polyantha (Serai kayu), Euodia redlevi (Tenggek burung) and Limnocharis flava (Sudu itik). The total phenolic content and antioxidant compounds were extracted using distilled water at three different extraction time (4, 8 and 12 hour). The extracts were then evaluated using Folin-Ciocalteu reagent for their total phenolic content and 2, 2-Diphenyl-1-picrylhydrazyl hydrate (DPPH) assay for their antioxidant compounds. Further analyzed was done using High Performance Liquid Chromatography (HPLC) to further verifying the ascorbic acid existence in the extracts. It was found L. flava showed the highest content of total phenolic compounds (15.01 mg gallic acid equivalent (GAE) per 1 g of sample) with the antioxidant capacity standing at 20.42 mg ascorbic acid equivalent (AAE) per 1 g of sample after 8 hour extraction. While, E. polyantha showed the highest value of antioxidant capacity (21.19 mg AAE/g) with total phenolic content of 9.99 mg GAE/g. In HPLC analysis, it was found that E. polyantha and L. flava after 8 hour extraction contained 0.7625 and 0.9745 mg AAE/g of ascorbic acid respectively.
format Undergraduates Project Papers
author Norhayantie, A.Rahman
author_facet Norhayantie, A.Rahman
author_sort Norhayantie, A.Rahman
title Total phenolic content and antioxidant activity of some Malaysian herbs
title_short Total phenolic content and antioxidant activity of some Malaysian herbs
title_full Total phenolic content and antioxidant activity of some Malaysian herbs
title_fullStr Total phenolic content and antioxidant activity of some Malaysian herbs
title_full_unstemmed Total phenolic content and antioxidant activity of some Malaysian herbs
title_sort total phenolic content and antioxidant activity of some malaysian herbs
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/7226/1/Total_phenolic_content_and_antioxidant_activity.pdf
http://umpir.ump.edu.my/id/eprint/7226/
_version_ 1702170073676906496
score 13.211869

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