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Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration

ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. Th...

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Main Author: Norhalmy, Mohd Nor
Format: Undergraduates Project Papers
Language:English
Published: 2006
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/559/1/Norhalmy_B._Mohd_Nor.pdf
http://umpir.ump.edu.my/id/eprint/559/
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spelling my.ump.umpir.5592021-06-03T05:17:49Z http://umpir.ump.edu.my/id/eprint/559/ Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration Norhalmy, Mohd Nor TP Chemical technology ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB. 2006-11 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/559/1/Norhalmy_B._Mohd_Nor.pdf Norhalmy, Mohd Nor (2006) Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Norhalmy, Mohd Nor
Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
description ABST ACT The use of waste as raw material is important for government economy and natural balance. The purpose of this work is to study the production of xylanase and CMCase enzyme by Aspergillus niger in solid state fermentation (SSF) using agricultural residues sugar cane bagasse as substrates. The study is focus on the effect of substrate concentration which is 1 g/L, 3g/L and 5 g/L. All the enzymes were incubating in temperature 37 oC and pH 5.0 for 30 minutes. The reaction was quenched by cooling on ice and the amount of reducing sugar was measured by dinitrosalicyclic (DNS) acid procedure with slight modification (Miller, 1965). The mixture was then incubated at 100 oC for 5 minutes. Subsequently, the reaction was stopped by cooling on ice. Distilled water was added into the final volume of 16 ml and the color intensity was measured at wavelength of 575 nm. The concentration of SCB affected the xylanase and CMCase activity. Xylanase and carboxymethylcellulase activity was higher at lower concentration hen using 3 g of SCB.
format Undergraduates Project Papers
author Norhalmy, Mohd Nor
author_facet Norhalmy, Mohd Nor
author_sort Norhalmy, Mohd Nor
title Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_short Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_full Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_fullStr Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_full_unstemmed Production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
title_sort production of cellulose enzyme from aspergillus niger using sugarcane bagasse; the effect of substrate concentration
publishDate 2006
url http://umpir.ump.edu.my/id/eprint/559/1/Norhalmy_B._Mohd_Nor.pdf
http://umpir.ump.edu.my/id/eprint/559/
_version_ 1702169986357788672
score 13.211869

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