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The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed

Production of extracellular protease refers to proteases produced from microorganisms and functions out of the cell in which it was originated. In this term, the microorganism was sought to be normal mesophilic bacteria. The experiment was conducted to evaluate the effects of temperature and agitati...

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Main Author: Mohd Fadli, Abdullah
Format: Undergraduates Project Papers
Language:English
Published: 2006
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/551/1/Mohd_Fadli_Bin_Abdullah.pdf
http://umpir.ump.edu.my/id/eprint/551/
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spelling my.ump.umpir.5512021-06-03T05:18:55Z http://umpir.ump.edu.my/id/eprint/551/ The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed Mohd Fadli, Abdullah TP Chemical technology Production of extracellular protease refers to proteases produced from microorganisms and functions out of the cell in which it was originated. In this term, the microorganism was sought to be normal mesophilic bacteria. The experiment was conducted to evaluate the effects of temperature and agitation speed of the incubator shaker towards the production of extracellular protease. Optimization of the enzyme production condition involving the two parameters was also made using statistical software. Typical methods conducted in previous researches were utilized in the experiments using different temperature and agitation speed. However, in previous researches, experiments were conducted through one-factor-at-a-time method (OF AT) but in this experiment, that method was overrun and designed beforehand using Minitab. The standard procedures of protease assay were carried out when experimenting. The bacteria were re-cultured to enable its functionality to serve its purpose. The results obtained proved that both parameters had significant effects if stood alone towards the production, however had to interact with each other due to randomization of the values implemented in the experiments. Simple analysis was not an option therefore the results were opted to be analyzed by Minitab and also the condition of protease production was optimized. 2006-11 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/551/1/Mohd_Fadli_Bin_Abdullah.pdf Mohd Fadli, Abdullah (2006) The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mohd Fadli, Abdullah
The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed
description Production of extracellular protease refers to proteases produced from microorganisms and functions out of the cell in which it was originated. In this term, the microorganism was sought to be normal mesophilic bacteria. The experiment was conducted to evaluate the effects of temperature and agitation speed of the incubator shaker towards the production of extracellular protease. Optimization of the enzyme production condition involving the two parameters was also made using statistical software. Typical methods conducted in previous researches were utilized in the experiments using different temperature and agitation speed. However, in previous researches, experiments were conducted through one-factor-at-a-time method (OF AT) but in this experiment, that method was overrun and designed beforehand using Minitab. The standard procedures of protease assay were carried out when experimenting. The bacteria were re-cultured to enable its functionality to serve its purpose. The results obtained proved that both parameters had significant effects if stood alone towards the production, however had to interact with each other due to randomization of the values implemented in the experiments. Simple analysis was not an option therefore the results were opted to be analyzed by Minitab and also the condition of protease production was optimized.
format Undergraduates Project Papers
author Mohd Fadli, Abdullah
author_facet Mohd Fadli, Abdullah
author_sort Mohd Fadli, Abdullah
title The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed
title_short The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed
title_full The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed
title_fullStr The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed
title_full_unstemmed The production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed
title_sort production of extracellular protease using bacillus subtilis : effect of temperature and agitation speed
publishDate 2006
url http://umpir.ump.edu.my/id/eprint/551/1/Mohd_Fadli_Bin_Abdullah.pdf
http://umpir.ump.edu.my/id/eprint/551/
_version_ 1702169983226740736
score 13.211869

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