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Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system

Jacalin is the major protein contained in the crude extract of jackfruit (Artocarpus heterophyllus) seed that specifically recognizes and binds reversibly to galactose. Conventionally, purification of jacalin is carried out using the tedious and costly chromatographic techniques. In this study, extr...

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Main Authors: S. F. S., Mohamad, F. M., Said, M. S. A., Munaim, S., Mohamad, W. M. A. W., Sulaiman
Format: Conference or Workshop Item
Language:English
Published: Institute of Physics Publishing 2020
Subjects:
QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/35951/1/Response%20surface%20optimization%20of%20the%20forward%20extraction%20of%20jacalin%20from%20jackfruit%20seeds%20using%20AOT_isooctane.pdf
http://umpir.ump.edu.my/id/eprint/35951/
https://doi.org/10.1088/1757-899X/716/1/012019
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spelling my.ump.umpir.359512022-12-28T06:45:13Z http://umpir.ump.edu.my/id/eprint/35951/ Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system S. F. S., Mohamad F. M., Said M. S. A., Munaim S., Mohamad W. M. A. W., Sulaiman QD Chemistry T Technology (General) TA Engineering (General). Civil engineering (General) TP Chemical technology Jacalin is the major protein contained in the crude extract of jackfruit (Artocarpus heterophyllus) seed that specifically recognizes and binds reversibly to galactose. Conventionally, purification of jacalin is carried out using the tedious and costly chromatographic techniques. In this study, extraction of jacalin from jackfruit seed crude extract were done using the sodium bis(2-ethylhexyl) sulfosuccinate (AOT)-based reverse micellar system. Reverse micellar extraction is an attractive alternative for downstream processing of various proteins. A successful reverse micellar extraction consists of two basic steps: forward and backward extraction. Forward extraction transfers a target protein from an aqueous solution into the reverse micellar solution, while backward extraction releases the protein from the reverse micelles structure into a new aqueous solution. The effects of the aqueous phase pH, NaCl concentration and AOT concentration on the forward extraction efficiency (FEE) are investigated using the response surface methodology (Box-Behnken Design). The main effects and interactions of the parameters are analyzed through the 3D surface plots. The optimum conditions for forward extraction were determined as follows: aqueous phase pH 4.58, 125 mM NaCl and 40 mM AOT. Under the optimal conditions, the FEE reached 88.04±1.30%, closer to 87.99% predicted by the model. The results indicated that AOT/isooctane reverse micelle system is effective in extracting jacalin from the jackfruit seed crude extract and verified the practicability of the BBD model for optimizing the main parameters in the forward extraction of jacalin. Institute of Physics Publishing 2020-02-11 Conference or Workshop Item PeerReviewed pdf en cc_by http://umpir.ump.edu.my/id/eprint/35951/1/Response%20surface%20optimization%20of%20the%20forward%20extraction%20of%20jacalin%20from%20jackfruit%20seeds%20using%20AOT_isooctane.pdf S. F. S., Mohamad and F. M., Said and M. S. A., Munaim and S., Mohamad and W. M. A. W., Sulaiman (2020) Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. In: IOP Conference Series: Materials Science and Engineering, The Third Bioprocessing and Biomanufacturing Symposium 2019, 9-10 April 2019 , Lumut, Perak, Malaysia. pp. 1-9., 716 (012019). ISSN 1757-8981 https://doi.org/10.1088/1757-899X/716/1/012019
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
spellingShingle QD Chemistry
T Technology (General)
TA Engineering (General). Civil engineering (General)
TP Chemical technology
S. F. S., Mohamad
F. M., Said
M. S. A., Munaim
S., Mohamad
W. M. A. W., Sulaiman
Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system
description Jacalin is the major protein contained in the crude extract of jackfruit (Artocarpus heterophyllus) seed that specifically recognizes and binds reversibly to galactose. Conventionally, purification of jacalin is carried out using the tedious and costly chromatographic techniques. In this study, extraction of jacalin from jackfruit seed crude extract were done using the sodium bis(2-ethylhexyl) sulfosuccinate (AOT)-based reverse micellar system. Reverse micellar extraction is an attractive alternative for downstream processing of various proteins. A successful reverse micellar extraction consists of two basic steps: forward and backward extraction. Forward extraction transfers a target protein from an aqueous solution into the reverse micellar solution, while backward extraction releases the protein from the reverse micelles structure into a new aqueous solution. The effects of the aqueous phase pH, NaCl concentration and AOT concentration on the forward extraction efficiency (FEE) are investigated using the response surface methodology (Box-Behnken Design). The main effects and interactions of the parameters are analyzed through the 3D surface plots. The optimum conditions for forward extraction were determined as follows: aqueous phase pH 4.58, 125 mM NaCl and 40 mM AOT. Under the optimal conditions, the FEE reached 88.04±1.30%, closer to 87.99% predicted by the model. The results indicated that AOT/isooctane reverse micelle system is effective in extracting jacalin from the jackfruit seed crude extract and verified the practicability of the BBD model for optimizing the main parameters in the forward extraction of jacalin.
format Conference or Workshop Item
author S. F. S., Mohamad
F. M., Said
M. S. A., Munaim
S., Mohamad
W. M. A. W., Sulaiman
author_facet S. F. S., Mohamad
F. M., Said
M. S. A., Munaim
S., Mohamad
W. M. A. W., Sulaiman
author_sort S. F. S., Mohamad
title Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system
title_short Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system
title_full Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system
title_fullStr Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system
title_full_unstemmed Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system
title_sort response surface optimization of the forward extraction of jacalin from jackfruit seeds using aot/isooctane reverse micellar system
publisher Institute of Physics Publishing
publishDate 2020
url http://umpir.ump.edu.my/id/eprint/35951/1/Response%20surface%20optimization%20of%20the%20forward%20extraction%20of%20jacalin%20from%20jackfruit%20seeds%20using%20AOT_isooctane.pdf
http://umpir.ump.edu.my/id/eprint/35951/
https://doi.org/10.1088/1757-899X/716/1/012019
_version_ 1753788573159522304
score 13.211869

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