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Pineapple leaf juice characterization and kinetic study on microbial growth inhibition

Microbial growth inhibitor (MGI) is crucial in preventing the spreaders of infection. Pineapple (Ananas comosus) leaf juice (PLJ) is chosen as an alternative MGI agent due to its phenolic compounds content. Phenolic compounds in PLJ were quantified by ultra-high performance liquid chromatography-qua...

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Main Authors: Amirah, Ya'acob, Norazwina, Zainol, Kamaliah, Abdul Samad
Format: Article
Language:English
Published: Penerbit UTM Press 2022
Subjects:
QD Chemistry
T Technology (General)
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/33573/1/Pineapple%20leaf%20juice%20characterization%20and%20kinetic%20study%20on%20microbial%20growth%20inhibition.pdf
http://umpir.ump.edu.my/id/eprint/33573/
https://doi.org/10.11113/jurnalteknologi.v84.16541
https://doi.org/10.11113/jurnalteknologi.v84.16541
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spelling my.ump.umpir.335732022-04-15T04:00:35Z http://umpir.ump.edu.my/id/eprint/33573/ Pineapple leaf juice characterization and kinetic study on microbial growth inhibition Amirah, Ya'acob Norazwina, Zainol Kamaliah, Abdul Samad QD Chemistry T Technology (General) TP Chemical technology Microbial growth inhibitor (MGI) is crucial in preventing the spreaders of infection. Pineapple (Ananas comosus) leaf juice (PLJ) is chosen as an alternative MGI agent due to its phenolic compounds content. Phenolic compounds in PLJ were quantified by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) analysis. Phenolic compounds identified were confirmed based on their molecular mass and fragmentation pattern. From PLJ characterization, seven phenolic compounds were identified, namely meliadanoside A, feralolide, kukoamine A, methyl-5-O-caffeoylquinate, stilbostemin D, octahydrocurcumin and agrimol C. As the time increased from 0 to 60 min, the phenolic compounds concentration-absorbance (Au) value decreased, indicating a decrease in the phenolic concentration. This is due to the inhibition of microbes by phenolic compounds in PLJ. The inhibition data obtained in microbial inhibition assay were plotted according to the Hill equation, where the kinetic constants (Vmax, K0.5, and n) were estimated. Meliadanoside A has the highest K0.5 value followed by feralolide, kukoamine A, methyl-5-O-caffeoylquinate, and stilbostemin D. Meliadanoside A displayed a sigmoidal behaviour with a Hill coefficient (n) greater than 1. Feralolide, kukoamine A, methyl-5-O-caffeoylquinate, and stilbostemin D corresponded to negative cooperativity with n values lower than 1. This study demonstrated that PLJ could be exploited as a natural plant source that acts as an effective microbial growth inhibitor. Thus, it becomes one of the greener alternatives MGI compared to other synthetic agents. Penerbit UTM Press 2022-03 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/33573/1/Pineapple%20leaf%20juice%20characterization%20and%20kinetic%20study%20on%20microbial%20growth%20inhibition.pdf Amirah, Ya'acob and Norazwina, Zainol and Kamaliah, Abdul Samad (2022) Pineapple leaf juice characterization and kinetic study on microbial growth inhibition. Jurnal Teknologi, 84 (2). 133 -144. ISSN 0127-9696 https://doi.org/10.11113/jurnalteknologi.v84.16541 https://doi.org/10.11113/jurnalteknologi.v84.16541
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
T Technology (General)
TP Chemical technology
spellingShingle QD Chemistry
T Technology (General)
TP Chemical technology
Amirah, Ya'acob
Norazwina, Zainol
Kamaliah, Abdul Samad
Pineapple leaf juice characterization and kinetic study on microbial growth inhibition
description Microbial growth inhibitor (MGI) is crucial in preventing the spreaders of infection. Pineapple (Ananas comosus) leaf juice (PLJ) is chosen as an alternative MGI agent due to its phenolic compounds content. Phenolic compounds in PLJ were quantified by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) analysis. Phenolic compounds identified were confirmed based on their molecular mass and fragmentation pattern. From PLJ characterization, seven phenolic compounds were identified, namely meliadanoside A, feralolide, kukoamine A, methyl-5-O-caffeoylquinate, stilbostemin D, octahydrocurcumin and agrimol C. As the time increased from 0 to 60 min, the phenolic compounds concentration-absorbance (Au) value decreased, indicating a decrease in the phenolic concentration. This is due to the inhibition of microbes by phenolic compounds in PLJ. The inhibition data obtained in microbial inhibition assay were plotted according to the Hill equation, where the kinetic constants (Vmax, K0.5, and n) were estimated. Meliadanoside A has the highest K0.5 value followed by feralolide, kukoamine A, methyl-5-O-caffeoylquinate, and stilbostemin D. Meliadanoside A displayed a sigmoidal behaviour with a Hill coefficient (n) greater than 1. Feralolide, kukoamine A, methyl-5-O-caffeoylquinate, and stilbostemin D corresponded to negative cooperativity with n values lower than 1. This study demonstrated that PLJ could be exploited as a natural plant source that acts as an effective microbial growth inhibitor. Thus, it becomes one of the greener alternatives MGI compared to other synthetic agents.
format Article
author Amirah, Ya'acob
Norazwina, Zainol
Kamaliah, Abdul Samad
author_facet Amirah, Ya'acob
Norazwina, Zainol
Kamaliah, Abdul Samad
author_sort Amirah, Ya'acob
title Pineapple leaf juice characterization and kinetic study on microbial growth inhibition
title_short Pineapple leaf juice characterization and kinetic study on microbial growth inhibition
title_full Pineapple leaf juice characterization and kinetic study on microbial growth inhibition
title_fullStr Pineapple leaf juice characterization and kinetic study on microbial growth inhibition
title_full_unstemmed Pineapple leaf juice characterization and kinetic study on microbial growth inhibition
title_sort pineapple leaf juice characterization and kinetic study on microbial growth inhibition
publisher Penerbit UTM Press
publishDate 2022
url http://umpir.ump.edu.my/id/eprint/33573/1/Pineapple%20leaf%20juice%20characterization%20and%20kinetic%20study%20on%20microbial%20growth%20inhibition.pdf
http://umpir.ump.edu.my/id/eprint/33573/
https://doi.org/10.11113/jurnalteknologi.v84.16541
https://doi.org/10.11113/jurnalteknologi.v84.16541
_version_ 1731225783223975936
score 13.211869

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