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Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry

The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis t...

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第一著者: Tan, Darlene Tzet Lene
フォーマット: Undergraduates Project Papers
言語:English
出版事項: 2017
主題:
T Technology (General)
オンライン･アクセス:http://umpir.ump.edu.my/id/eprint/24521/1/Extraction%20of%20propolis%20by%20using%20water%20and%20evaluation%20by%20TLC.pdf
http://umpir.ump.edu.my/id/eprint/24521/
http://ecollib.ump.edu.my/36216/
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spelling my.ump.umpir.245212023-11-20T07:56:59Z http://umpir.ump.edu.my/id/eprint/24521/ Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry Tan, Darlene Tzet Lene T Technology (General) The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis to solvent ratio was determined by increasing the ratio from 1:5, 1:10 to 1:15 where the extraction yield of extract were 5.95%, 9.05% and 7.16% respectively. Larger ratio increases extract yield, however, extract yield at ratio larger 1:10 will not further increase percentage. Analysis by UV-Vis Spectrophotometry was carried out to determine the total flavone, flavonols and total phenolic contents in Water Extracted Propolis (WEP). Both contents showed a highest concentration in the 1:5 ratio, which were 3.33 mg Quercetin Equivalent (QE)/g and 6.12 mg Gallic Acid Equivalent (GAE)/g respectively. TLC analysis detected caffeic acid components in WEP at 2017-12 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24521/1/Extraction%20of%20propolis%20by%20using%20water%20and%20evaluation%20by%20TLC.pdf Tan, Darlene Tzet Lene (2017) Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry. Faculty of Engineering Technology, Universiti Malaysia Pahang. http://ecollib.ump.edu.my/36216/
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Tan, Darlene Tzet Lene
Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry
description The objective of this research was to perform extraction of propolis using water as extraction solvent and analyze the propolis extract by using Thin Layer Chromatography (TLC), High Performance liquid Chromatography (HPLC) and Ultraviolet–Visible (UV-Vis) Spectrophotometry. The effect of propolis to solvent ratio was determined by increasing the ratio from 1:5, 1:10 to 1:15 where the extraction yield of extract were 5.95%, 9.05% and 7.16% respectively. Larger ratio increases extract yield, however, extract yield at ratio larger 1:10 will not further increase percentage. Analysis by UV-Vis Spectrophotometry was carried out to determine the total flavone, flavonols and total phenolic contents in Water Extracted Propolis (WEP). Both contents showed a highest concentration in the 1:5 ratio, which were 3.33 mg Quercetin Equivalent (QE)/g and 6.12 mg Gallic Acid Equivalent (GAE)/g respectively. TLC analysis detected caffeic acid components in WEP at
format Undergraduates Project Papers
author Tan, Darlene Tzet Lene
author_facet Tan, Darlene Tzet Lene
author_sort Tan, Darlene Tzet Lene
title Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry
title_short Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry
title_full Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry
title_fullStr Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry
title_full_unstemmed Extraction of propolis by using water and evaluation by TLC, HPLC and UV VIS spectrophotometry
title_sort extraction of propolis by using water and evaluation by tlc, hplc and uv vis spectrophotometry
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/24521/1/Extraction%20of%20propolis%20by%20using%20water%20and%20evaluation%20by%20TLC.pdf
http://umpir.ump.edu.my/id/eprint/24521/
http://ecollib.ump.edu.my/36216/
_version_ 1822920698584104960
score 13.250246

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