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Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes

This study was concerned with the assessment of antioxidant activity and chemical composition of extracts made from nine legume seeds. The legume seeds include : Lablab purpueus (Lab-lab bean), Phaseolus vulgaris (Kidney bean), Vigna radiata (Mung bean, green seed coat variety), Cicer arietinum (Chi...

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Main Authors: M., Khan, S. A., Ali-Elhussain, T., Ghous, F., Kormin, A., Ajit, T., Ahmad, M. K., Muhammadi, T., Batool, F., Akhtar, I., Ahmed, A. Z., Sulaiman
Format: Conference or Workshop Item
Language:English
English
Published: Universiti Malaysia Pahang 2018
Subjects:
T Technology (General)
Online Access:http://umpir.ump.edu.my/id/eprint/22424/1/43.%20Assessment%20of%20in-vitro%20antioxidant%20capacity%20and%20polyphenolic.pdf
http://umpir.ump.edu.my/id/eprint/22424/2/43.1%20Assessment%20of%20in-vitro%20antioxidant%20capacity%20and%20polyphenolic.pdf
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spelling my.ump.umpir.224242018-12-13T01:24:03Z http://umpir.ump.edu.my/id/eprint/22424/ Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes M., Khan S. A., Ali-Elhussain T., Ghous F., Kormin A., Ajit T., Ahmad M. K., Muhammadi T., Batool F., Akhtar I., Ahmed A. Z., Sulaiman T Technology (General) This study was concerned with the assessment of antioxidant activity and chemical composition of extracts made from nine legume seeds. The legume seeds include : Lablab purpueus (Lab-lab bean), Phaseolus vulgaris (Kidney bean), Vigna radiata (Mung bean, green seed coat variety), Cicer arietinum (Chick pea, dark brown seed coat variety), Vigna radiate (Mung bean, red seed coat variety), Vigna unguiculata (Cowpea, Black eyed bean), Cicer arietinum (Chick pea, white seed coat variety), Trigonella foenumgraecum (Fenugreek), and Phaseolus vulgaris (Black bean). Two methods were used to evaluate antioxidant activity of ethanol extracts of these selected plants: β-carotene bleaching assay and 1,1-diphenyl-2-picrylhydrazyl radical method (DPPH). DPPH was also used as a TLC spray to detect separated antioxidant compounds. Ethanol extracts of the five legume seeds showed antioxidant activity with chick pea (Cicer arietinum) exhibiting the highest activity (Fernandez et al.,, 2009). Germination did not increased antioxidant activity as measured by the β-carotene method, in five legumes. However, TLC bioautography clearly indicated that at least four compounds with radical scavenging activity have been biosynthesized during the germination of chick pea seeds. The biosynthesized compounds were more polar compared to those present in ungerminated seeds. This broadens the solubility spectrum of chickpea antioxidants, an advantage for the food industry (Milos and Makhota, 2012). The potential of commercially using legume flours in processed baked and meat products are indicated, adding antioxidant activity to other functionalities. Synergistic studies showed that the antioxidant activity of chick pea could be synergistically increased by addition of the extract of roots of G. Gynandra (Chew et al., 2009) The AlCl3-method was found to be unsuitable for the determination of total flavonoids. Universiti Malaysia Pahang 2018-07 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22424/1/43.%20Assessment%20of%20in-vitro%20antioxidant%20capacity%20and%20polyphenolic.pdf pdf en http://umpir.ump.edu.my/id/eprint/22424/2/43.1%20Assessment%20of%20in-vitro%20antioxidant%20capacity%20and%20polyphenolic.pdf M., Khan and S. A., Ali-Elhussain and T., Ghous and F., Kormin and A., Ajit and T., Ahmad and M. K., Muhammadi and T., Batool and F., Akhtar and I., Ahmed and A. Z., Sulaiman (2018) Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes. In: 4th International Conference of Chemical Engineering & Industrial Biotechnology, 1-2 August 2018 , Seri Pacific Hotel Kuala Lumpur. pp. 1-18.. (Unpublished)
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic T Technology (General)
spellingShingle T Technology (General)
M., Khan
S. A., Ali-Elhussain
T., Ghous
F., Kormin
A., Ajit
T., Ahmad
M. K., Muhammadi
T., Batool
F., Akhtar
I., Ahmed
A. Z., Sulaiman
Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes
description This study was concerned with the assessment of antioxidant activity and chemical composition of extracts made from nine legume seeds. The legume seeds include : Lablab purpueus (Lab-lab bean), Phaseolus vulgaris (Kidney bean), Vigna radiata (Mung bean, green seed coat variety), Cicer arietinum (Chick pea, dark brown seed coat variety), Vigna radiate (Mung bean, red seed coat variety), Vigna unguiculata (Cowpea, Black eyed bean), Cicer arietinum (Chick pea, white seed coat variety), Trigonella foenumgraecum (Fenugreek), and Phaseolus vulgaris (Black bean). Two methods were used to evaluate antioxidant activity of ethanol extracts of these selected plants: β-carotene bleaching assay and 1,1-diphenyl-2-picrylhydrazyl radical method (DPPH). DPPH was also used as a TLC spray to detect separated antioxidant compounds. Ethanol extracts of the five legume seeds showed antioxidant activity with chick pea (Cicer arietinum) exhibiting the highest activity (Fernandez et al.,, 2009). Germination did not increased antioxidant activity as measured by the β-carotene method, in five legumes. However, TLC bioautography clearly indicated that at least four compounds with radical scavenging activity have been biosynthesized during the germination of chick pea seeds. The biosynthesized compounds were more polar compared to those present in ungerminated seeds. This broadens the solubility spectrum of chickpea antioxidants, an advantage for the food industry (Milos and Makhota, 2012). The potential of commercially using legume flours in processed baked and meat products are indicated, adding antioxidant activity to other functionalities. Synergistic studies showed that the antioxidant activity of chick pea could be synergistically increased by addition of the extract of roots of G. Gynandra (Chew et al., 2009) The AlCl3-method was found to be unsuitable for the determination of total flavonoids.
format Conference or Workshop Item
author M., Khan
S. A., Ali-Elhussain
T., Ghous
F., Kormin
A., Ajit
T., Ahmad
M. K., Muhammadi
T., Batool
F., Akhtar
I., Ahmed
A. Z., Sulaiman
author_facet M., Khan
S. A., Ali-Elhussain
T., Ghous
F., Kormin
A., Ajit
T., Ahmad
M. K., Muhammadi
T., Batool
F., Akhtar
I., Ahmed
A. Z., Sulaiman
author_sort M., Khan
title Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes
title_short Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes
title_full Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes
title_fullStr Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes
title_full_unstemmed Assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes
title_sort assessment of in-vitro antioxidant capacity and polyphenolic composition of frequently used legumes
publisher Universiti Malaysia Pahang
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/22424/1/43.%20Assessment%20of%20in-vitro%20antioxidant%20capacity%20and%20polyphenolic.pdf
http://umpir.ump.edu.my/id/eprint/22424/2/43.1%20Assessment%20of%20in-vitro%20antioxidant%20capacity%20and%20polyphenolic.pdf
http://umpir.ump.edu.my/id/eprint/22424/
_version_ 1643669377381302272
score 13.211869

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