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Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support

This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot...

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Main Authors: Ponnarasy, Ganasen, Khan, Maksudur R., M., Abul Kalam, Mohd Sabri, Mahmud
Format: Article
Language:English
English
English
Published: Springer Berlin Heidelberg 2014
Subjects:
TA Engineering (General). Civil engineering (General)
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/12375/1/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis%20.pdf
http://umpir.ump.edu.my/id/eprint/12375/2/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis.pdf
http://umpir.ump.edu.my/id/eprint/12375/13/Effect%20Of%20Visible%20Light%20On%20Catalytic%20Hydrolysis%20Of%20P-Nitrophenyl%20Palmitate%20By%20The%20Pseudomonas%20Cepacia%20Lipase%20Immobilized%20On%20Sol%E2%80%93Gel%20Support.pdf
http://umpir.ump.edu.my/id/eprint/12375/
http://dx.doi.org/10.1007/s00449-014-1213-6
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spelling my.ump.umpir.123752017-08-17T03:07:53Z http://umpir.ump.edu.my/id/eprint/12375/ Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support Ponnarasy, Ganasen Khan, Maksudur R. M., Abul Kalam Mohd Sabri, Mahmud TA Engineering (General). Civil engineering (General) TP Chemical technology This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction. Springer Berlin Heidelberg 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12375/1/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis%20.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12375/2/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/12375/13/Effect%20Of%20Visible%20Light%20On%20Catalytic%20Hydrolysis%20Of%20P-Nitrophenyl%20Palmitate%20By%20The%20Pseudomonas%20Cepacia%20Lipase%20Immobilized%20On%20Sol%E2%80%93Gel%20Support.pdf Ponnarasy, Ganasen and Khan, Maksudur R. and M., Abul Kalam and Mohd Sabri, Mahmud (2014) Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support. Bioprocess and Biosystems Engineering, 37 (11). pp. 2353-2359. ISSN 1615-7591 (print); 1615-7605 (online) http://dx.doi.org/10.1007/s00449-014-1213-6 DOI 10.1007/s00449-014-1213-6
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
English
topic TA Engineering (General). Civil engineering (General)
TP Chemical technology
spellingShingle TA Engineering (General). Civil engineering (General)
TP Chemical technology
Ponnarasy, Ganasen
Khan, Maksudur R.
M., Abul Kalam
Mohd Sabri, Mahmud
Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support
description This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction.
format Article
author Ponnarasy, Ganasen
Khan, Maksudur R.
M., Abul Kalam
Mohd Sabri, Mahmud
author_facet Ponnarasy, Ganasen
Khan, Maksudur R.
M., Abul Kalam
Mohd Sabri, Mahmud
author_sort Ponnarasy, Ganasen
title Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support
title_short Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support
title_full Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support
title_fullStr Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support
title_full_unstemmed Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support
title_sort effect of visible light on catalytic hydrolysis of p-nitrophenyl palmitate by the pseudomonas cepacia lipase immobilized on sol–gel support
publisher Springer Berlin Heidelberg
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/12375/1/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis%20.pdf
http://umpir.ump.edu.my/id/eprint/12375/2/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis.pdf
http://umpir.ump.edu.my/id/eprint/12375/13/Effect%20Of%20Visible%20Light%20On%20Catalytic%20Hydrolysis%20Of%20P-Nitrophenyl%20Palmitate%20By%20The%20Pseudomonas%20Cepacia%20Lipase%20Immobilized%20On%20Sol%E2%80%93Gel%20Support.pdf
http://umpir.ump.edu.my/id/eprint/12375/
http://dx.doi.org/10.1007/s00449-014-1213-6
_version_ 1643666896773447680
score 13.211869

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