Effect of Visible Light on Catalytic Hydrolysis of P-nitrophenyl Palmitate by the Pseudomonas Cepacia Lipase Immobilized on Sol–Gel Support
This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Springer Berlin Heidelberg
2014
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/12375/1/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis%20.pdf http://umpir.ump.edu.my/id/eprint/12375/2/Effect%20of%20visible%20light%20on%20catalytic%20hydrolysis.pdf http://umpir.ump.edu.my/id/eprint/12375/13/Effect%20Of%20Visible%20Light%20On%20Catalytic%20Hydrolysis%20Of%20P-Nitrophenyl%20Palmitate%20By%20The%20Pseudomonas%20Cepacia%20Lipase%20Immobilized%20On%20Sol%E2%80%93Gel%20Support.pdf http://umpir.ump.edu.my/id/eprint/12375/ http://dx.doi.org/10.1007/s00449-014-1213-6 |
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| Summary: | This paper demonstrates Pseudomonas cepacia lipase catalyzed hydrolysis of p-nitrophenyl palmitate under irradiation of light with wavelengths of 250–750 nm. The reaction follows Michaelis–Menten Kinetics and the light irradiation increases the overall rate of hydrolysis. Using Lineweaver–Burk plot K M and V max values for the reaction in presence of light are found to be 39.07 and 66.67 mM/min/g, respectively; while for the same reaction under dark condition, the values are 7.08 and 10.21 mM/min/g. The linear form of enzyme dependent rate of reaction confirms that no mass-transfer limitations are present and the reaction is a kinetically controlled enzymatic reaction. |
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