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Bioethanol production by baker's yeast in opts - effect of temperature and ph

This paper presented bioethanol production in oil palm trunk sap (OPTS) by using baker's yeast - effect of temperature and pH. Baker's yeast as known as Saccharomyces cerevisiae. Due to rapid growth in population and industrialization, ecofriendly bioethanol demand is rising rapidly w...

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Main Author: Farah Fadhilah, Fahrur Razi
Format: Undergraduates Project Papers
Language:English
Published: 2014
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/10186/1/FARAH%20FADHILAH%20BINTI%20FAHRUR%20RAZI.PDF
http://umpir.ump.edu.my/id/eprint/10186/
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spelling my.ump.umpir.101862021-07-26T08:05:50Z http://umpir.ump.edu.my/id/eprint/10186/ Bioethanol production by baker's yeast in opts - effect of temperature and ph Farah Fadhilah, Fahrur Razi TP Chemical technology This paper presented bioethanol production in oil palm trunk sap (OPTS) by using baker's yeast - effect of temperature and pH. Baker's yeast as known as Saccharomyces cerevisiae. Due to rapid growth in population and industrialization, ecofriendly bioethanol demand is rising rapidly wbrldwide. Bioethanol offers more advantages than fossil fuel since it provides renewable and sustainable sources of energy. The most commonly. employed methods for bioethanol generation are fermentation using baker's yeast (Ahrnad et al., 2011). Baker's yeast is commonly used because it is the cheapest strain available for conversion of V V biomass substrate and produces high yield of ethanol. Due to the cost of raw materials, the cheap renewable agricultural wastes are chosen as alternative substrates to produce ethanol. This study is focused on studying the parameters of temperature, (27 t-39 °C) and pH (3-7) for bioethanol production from oil palm trunk sap (OPTS) by baker's yeast, Saccharomyces cerevisiae. The yeast is grown in nutrient broth (NB) and ferment in oil palm trunk sap (OPTS) medium at- different conditions. By analyzing temperature and pH parameters, specific growth rate, glucose consumption rate, specific ethanol production- and production yield of ethanol can be determined. The analytical technique involved is high-performance liquid chromatography (HPLC) for glucose consumption and ethanol production while cell dry weight (CDW) is determined for growth. From this study, the maximum pH and temperature for bioethanol production were determined to be pH. 6 and 30 °C respectively. The maximum concentration of bioethanol for pH was 34.4 g/L while maximum concentration of bioethanol for temperature, it was 43.4 g/L. In addition, the maximum bioethanol production yield was 0.6 g/g at pH 6 and the maximum bioethanol production yield at temperature at 33 °C was 0.46 g/g. The maximum production yield was contrast with to maximum bioethanol production. This is because the total sugar concentration at 30 °C was too high compared to others: This level might exceed sugar critical level and cause substrate, inhibition to occur. This factor inhibited the ethanol productivity to be produced in high level. Last but not least, it could be concluded that the optimum pH and temperature for growth and production of bioethanol using S. cerevisiae in OPTS were in the range of pH 5-6 and temperature 30 -36°C. 2014-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/10186/1/FARAH%20FADHILAH%20BINTI%20FAHRUR%20RAZI.PDF Farah Fadhilah, Fahrur Razi (2014) Bioethanol production by baker's yeast in opts - effect of temperature and ph. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Farah Fadhilah, Fahrur Razi
Bioethanol production by baker's yeast in opts - effect of temperature and ph
description This paper presented bioethanol production in oil palm trunk sap (OPTS) by using baker's yeast - effect of temperature and pH. Baker's yeast as known as Saccharomyces cerevisiae. Due to rapid growth in population and industrialization, ecofriendly bioethanol demand is rising rapidly wbrldwide. Bioethanol offers more advantages than fossil fuel since it provides renewable and sustainable sources of energy. The most commonly. employed methods for bioethanol generation are fermentation using baker's yeast (Ahrnad et al., 2011). Baker's yeast is commonly used because it is the cheapest strain available for conversion of V V biomass substrate and produces high yield of ethanol. Due to the cost of raw materials, the cheap renewable agricultural wastes are chosen as alternative substrates to produce ethanol. This study is focused on studying the parameters of temperature, (27 t-39 °C) and pH (3-7) for bioethanol production from oil palm trunk sap (OPTS) by baker's yeast, Saccharomyces cerevisiae. The yeast is grown in nutrient broth (NB) and ferment in oil palm trunk sap (OPTS) medium at- different conditions. By analyzing temperature and pH parameters, specific growth rate, glucose consumption rate, specific ethanol production- and production yield of ethanol can be determined. The analytical technique involved is high-performance liquid chromatography (HPLC) for glucose consumption and ethanol production while cell dry weight (CDW) is determined for growth. From this study, the maximum pH and temperature for bioethanol production were determined to be pH. 6 and 30 °C respectively. The maximum concentration of bioethanol for pH was 34.4 g/L while maximum concentration of bioethanol for temperature, it was 43.4 g/L. In addition, the maximum bioethanol production yield was 0.6 g/g at pH 6 and the maximum bioethanol production yield at temperature at 33 °C was 0.46 g/g. The maximum production yield was contrast with to maximum bioethanol production. This is because the total sugar concentration at 30 °C was too high compared to others: This level might exceed sugar critical level and cause substrate, inhibition to occur. This factor inhibited the ethanol productivity to be produced in high level. Last but not least, it could be concluded that the optimum pH and temperature for growth and production of bioethanol using S. cerevisiae in OPTS were in the range of pH 5-6 and temperature 30 -36°C.
format Undergraduates Project Papers
author Farah Fadhilah, Fahrur Razi
author_facet Farah Fadhilah, Fahrur Razi
author_sort Farah Fadhilah, Fahrur Razi
title Bioethanol production by baker's yeast in opts - effect of temperature and ph
title_short Bioethanol production by baker's yeast in opts - effect of temperature and ph
title_full Bioethanol production by baker's yeast in opts - effect of temperature and ph
title_fullStr Bioethanol production by baker's yeast in opts - effect of temperature and ph
title_full_unstemmed Bioethanol production by baker's yeast in opts - effect of temperature and ph
title_sort bioethanol production by baker's yeast in opts - effect of temperature and ph
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/10186/1/FARAH%20FADHILAH%20BINTI%20FAHRUR%20RAZI.PDF
http://umpir.ump.edu.my/id/eprint/10186/
_version_ 1706957208150867968
score 13.211869

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