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Gender identification of domesticated chicken using a PCR-based method

The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently, the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus, a PCR based gender determination protocol was established to obtain a higher accuracy rate than...

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Main Authors: Richard Teh Swee Aun, Jayaraj V.K
Format: Non-Indexed Article
Published: 2010
Online Access:http://discol.umk.edu.my/id/eprint/7667/
http://www.pertanika.upm.edu.my/Pertanika%20ARCHIVES/JTAS/2010/JTAS%20Vol.%2033%20(2)%20Aug.%202010%20.pdf
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spelling my.umk.eprints.76672022-05-23T10:21:34Z http://discol.umk.edu.my/id/eprint/7667/ Gender identification of domesticated chicken using a PCR-based method Richard Teh Swee Aun Jayaraj V.K The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently, the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus, a PCR based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing methods. Feathers of a day up to a week old chicks were collected for the molecular analysis using the PCR targeting the CHD-W and CHD-Z genes in the chicken’s chromosome Z and chromosome W, respectively. The results of this study showed that the PCR based gender determination protocol was a sensitive and accurate method for determining the gender of monomorphic chicks as compared to the conventional sexing method. 2010 Non-Indexed Article NonPeerReviewed Richard Teh Swee Aun and Jayaraj V.K (2010) Gender identification of domesticated chicken using a PCR-based method. Pertanika Journal of Tropical Agricultural Science, 33 (2). pp. 329-336. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20ARCHIVES/JTAS/2010/JTAS%20Vol.%2033%20(2)%20Aug.%202010%20.pdf
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently, the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus, a PCR based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing methods. Feathers of a day up to a week old chicks were collected for the molecular analysis using the PCR targeting the CHD-W and CHD-Z genes in the chicken’s chromosome Z and chromosome W, respectively. The results of this study showed that the PCR based gender determination protocol was a sensitive and accurate method for determining the gender of monomorphic chicks as compared to the conventional sexing method.
format Non-Indexed Article
author Richard Teh Swee Aun
Jayaraj V.K
spellingShingle Richard Teh Swee Aun
Jayaraj V.K
Gender identification of domesticated chicken using a PCR-based method
author_facet Richard Teh Swee Aun
Jayaraj V.K
author_sort Richard Teh Swee Aun
title Gender identification of domesticated chicken using a PCR-based method
title_short Gender identification of domesticated chicken using a PCR-based method
title_full Gender identification of domesticated chicken using a PCR-based method
title_fullStr Gender identification of domesticated chicken using a PCR-based method
title_full_unstemmed Gender identification of domesticated chicken using a PCR-based method
title_sort gender identification of domesticated chicken using a pcr-based method
publishDate 2010
url http://discol.umk.edu.my/id/eprint/7667/
http://www.pertanika.upm.edu.my/Pertanika%20ARCHIVES/JTAS/2010/JTAS%20Vol.%2033%20(2)%20Aug.%202010%20.pdf
_version_ 1763303873941864448
score 13.211869

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