Gender identification of domesticated chicken using a PCR-based method
The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently, the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus, a PCR based gender determination protocol was established to obtain a higher accuracy rate than...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Non-Indexed Article |
| Published: |
2010
|
| Online Access: | http://discol.umk.edu.my/id/eprint/7667/ http://www.pertanika.upm.edu.my/Pertanika%20ARCHIVES/JTAS/2010/JTAS%20Vol.%2033%20(2)%20Aug.%202010%20.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The monomorphism of newly hatched chicks poses a problem for farmers who need to separate them. Currently,
the most widely used technique is the cloacal sexing method which has a low accuracy rate. Thus, a PCR
based gender determination protocol was established to obtain a higher accuracy rate than conventional sexing
methods. Feathers of a day up to a week old chicks were collected for the molecular analysis using the PCR
targeting the CHD-W and CHD-Z genes in the chicken’s chromosome Z and chromosome W, respectively. The
results of this study showed that the PCR based gender determination protocol was a sensitive and accurate
method for determining the gender of monomorphic chicks as compared to the conventional sexing method. |
|---|