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Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system.

Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate Aspergillus niger USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30∘...

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Main Authors: C.K.Lee, Darah Ibrahim, Ibrahim Che Omar
Format: Non-Indexed Article
Published: 2011
Online Access:http://discol.umk.edu.my/id/eprint/7644/
http://www.hindawi.com/journals/btri/2011/658493/
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spelling my.umk.eprints.76442022-05-23T10:21:27Z http://discol.umk.edu.my/id/eprint/7644/ Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system. C.K.Lee Darah Ibrahim Ibrahim Che Omar Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate Aspergillus niger USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30∘C; aeration at 4 L/h·g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 U/g of Filter paper activity (FPase) was obtained. At the same time, comparative studies of the enzymes production under the same SSF conditions indicated that FPase produced by A. niger USM AI 1 was about 35.3% higher compared to Trichoderma reesei. This shows that the performance of this newly designed SSF bioreactor is acceptable and potentially used as prototype for larger-scale bioreactor design 2011 Non-Indexed Article NonPeerReviewed C.K.Lee and Darah Ibrahim and Ibrahim Che Omar (2011) Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system. Biotechnology Research International, 2011. pp. 1-6. ISSN 2090-3146 http://www.hindawi.com/journals/btri/2011/658493/ 10.4061/2011/658493 10.4061/2011/658493 10.4061/2011/658493
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate Aspergillus niger USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30∘C; aeration at 4 L/h·g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 U/g of Filter paper activity (FPase) was obtained. At the same time, comparative studies of the enzymes production under the same SSF conditions indicated that FPase produced by A. niger USM AI 1 was about 35.3% higher compared to Trichoderma reesei. This shows that the performance of this newly designed SSF bioreactor is acceptable and potentially used as prototype for larger-scale bioreactor design
format Non-Indexed Article
author C.K.Lee
Darah Ibrahim
Ibrahim Che Omar
spellingShingle C.K.Lee
Darah Ibrahim
Ibrahim Che Omar
Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system.
author_facet C.K.Lee
Darah Ibrahim
Ibrahim Che Omar
author_sort C.K.Lee
title Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system.
title_short Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system.
title_full Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system.
title_fullStr Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system.
title_full_unstemmed Production and optimization of cellulase enzyme using aspergillus niger USM A1 and comparison with trichoderma reesei via solid state fermentation system.
title_sort production and optimization of cellulase enzyme using aspergillus niger usm a1 and comparison with trichoderma reesei via solid state fermentation system.
publishDate 2011
url http://discol.umk.edu.my/id/eprint/7644/
http://www.hindawi.com/journals/btri/2011/658493/
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score 13.211869

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