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Rapeseed meal hydrolysate as substrate for microbial astaxanthin production

Rapeseed meal, a by-product of oil processing industry, was evaluated as a substrate for astaxanthin production by the yeast Xanthophyllomyces dendrorhous DSMZ 5626. Four commercial enzymes were tested at different concentrations (1–15%, v/v) for their ability to break down the cellulosic and hemice...

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Main Authors: Tuan Harith, Zuharlida, Charalampopoulos, Dimitris, Chatzifragkou, Afroditi
Format: Indexed Article
Published: Elsevier 2019
Online Access:http://discol.umk.edu.my/id/eprint/7291/
https://www.sciencedirect.com/science/article/pii/S1369703X19302657
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spelling my.umk.eprints.72912022-05-23T09:55:39Z http://discol.umk.edu.my/id/eprint/7291/ Rapeseed meal hydrolysate as substrate for microbial astaxanthin production Tuan Harith, Zuharlida Charalampopoulos, Dimitris Chatzifragkou, Afroditi Rapeseed meal, a by-product of oil processing industry, was evaluated as a substrate for astaxanthin production by the yeast Xanthophyllomyces dendrorhous DSMZ 5626. Four commercial enzymes were tested at different concentrations (1–15%, v/v) for their ability to break down the cellulosic and hemicellulosic compounds of rapeseed meal into fermentable sugars. Viscozyme® L and cellulase demonstrated the highest glucose recovery yields (47–52%, w/w for 15% (v/v) of enzyme loading) with 7–11 g/l of net glucose released in the hydrolysates. Pectinase and Accellerase® hydrolysates supported the best cell growth and astaxanthin production in batch shake flask cultures, with maximum biomass of 26 g/l and 15 g/l, respectively, and astaxanthin yields (YP/X) of 258–332 μg per g of biomass. In batch bioreactor trials, pectinase hydrolysates resulted in high biomass (42 g/l) and astaxanthin production (11 mg/l) aided by the presence of glycerol (originating from the enzyme formulation) which served as additional energy and carbon source. Finally, simple glass beads disruption lead into satisfactory astaxanthin extraction (95%, w/w) in acetone. The findings of this study generate knowledge towards scale-up potential of microbial astaxanthin production using rapeseed meal hydrolysate as fermentation feedstock. Elsevier 2019 Indexed Article NonPeerReviewed Tuan Harith, Zuharlida and Charalampopoulos, Dimitris and Chatzifragkou, Afroditi (2019) Rapeseed meal hydrolysate as substrate for microbial astaxanthin production. Biochemical Engineering Journal, 151 (15). ISSN 1369-703X https://www.sciencedirect.com/science/article/pii/S1369703X19302657
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Rapeseed meal, a by-product of oil processing industry, was evaluated as a substrate for astaxanthin production by the yeast Xanthophyllomyces dendrorhous DSMZ 5626. Four commercial enzymes were tested at different concentrations (1–15%, v/v) for their ability to break down the cellulosic and hemicellulosic compounds of rapeseed meal into fermentable sugars. Viscozyme® L and cellulase demonstrated the highest glucose recovery yields (47–52%, w/w for 15% (v/v) of enzyme loading) with 7–11 g/l of net glucose released in the hydrolysates. Pectinase and Accellerase® hydrolysates supported the best cell growth and astaxanthin production in batch shake flask cultures, with maximum biomass of 26 g/l and 15 g/l, respectively, and astaxanthin yields (YP/X) of 258–332 μg per g of biomass. In batch bioreactor trials, pectinase hydrolysates resulted in high biomass (42 g/l) and astaxanthin production (11 mg/l) aided by the presence of glycerol (originating from the enzyme formulation) which served as additional energy and carbon source. Finally, simple glass beads disruption lead into satisfactory astaxanthin extraction (95%, w/w) in acetone. The findings of this study generate knowledge towards scale-up potential of microbial astaxanthin production using rapeseed meal hydrolysate as fermentation feedstock.
format Indexed Article
author Tuan Harith, Zuharlida
Charalampopoulos, Dimitris
Chatzifragkou, Afroditi
spellingShingle Tuan Harith, Zuharlida
Charalampopoulos, Dimitris
Chatzifragkou, Afroditi
Rapeseed meal hydrolysate as substrate for microbial astaxanthin production
author_facet Tuan Harith, Zuharlida
Charalampopoulos, Dimitris
Chatzifragkou, Afroditi
author_sort Tuan Harith, Zuharlida
title Rapeseed meal hydrolysate as substrate for microbial astaxanthin production
title_short Rapeseed meal hydrolysate as substrate for microbial astaxanthin production
title_full Rapeseed meal hydrolysate as substrate for microbial astaxanthin production
title_fullStr Rapeseed meal hydrolysate as substrate for microbial astaxanthin production
title_full_unstemmed Rapeseed meal hydrolysate as substrate for microbial astaxanthin production
title_sort rapeseed meal hydrolysate as substrate for microbial astaxanthin production
publisher Elsevier
publishDate 2019
url http://discol.umk.edu.my/id/eprint/7291/
https://www.sciencedirect.com/science/article/pii/S1369703X19302657
_version_ 1763303820217024512
score 13.211869

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