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Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems

There are a lots of antioxidant food are thermo-labile. The effect of the decoction of Plectranthus amboinicus (P. amboinicus) stems which were rarely utilized by people had been study in this research with three difference solvents (ethanol, ethyl acetate, and water) extraction respectively. The de...

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Main Author: Tan, Soon Khing
Format: Undergraduate Final Project Report
Published: 2015
Online Access:http://discol.umk.edu.my/id/eprint/6763/
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spelling my.umk.eprints.67632022-05-23T08:49:12Z http://discol.umk.edu.my/id/eprint/6763/ Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems Tan, Soon Khing There are a lots of antioxidant food are thermo-labile. The effect of the decoction of Plectranthus amboinicus (P. amboinicus) stems which were rarely utilized by people had been study in this research with three difference solvents (ethanol, ethyl acetate, and water) extraction respectively. The decoction time were divided into 0, 30, 60, 90 and 120 minutes. The powdered water extracts were obtained by freeze dried of the filtrate of decoction. The remaining biomass were further extract by ethyl acetate followed by ethanol. The antioxidant activity of P.amboinicus stems was determined by DPPH (1, 1-diphenyl-2-picrylhydrazine) scavenging assay. The total phenolic content of the P.amboinicus stems were expressed as gallic acid equivalents using acid standard curve of gallic acid (R²=0.9834. The qualitative analysis of thin layer chromatography (TLC) and DPPH autobiography were used to support the quantitative analysis as the evidence. The percentage of scavenging activity showed the ethyl acetate extracted of P.amboinicus has the highest antioxidant activity with the lowest (1C50) value 32.58 ± 0.014 at 120 minutes decoction. There are no significant difference (P<0.05) between the decoction of time and the antioxidant activity. Therefore, the decoction are not deteriorate the antioxidant activity of P.amboinicus stems. The TLC-DPPH autobiography were showed similar pattern within the three difference solvents. 2015 Undergraduate Final Project Report NonPeerReviewed Tan, Soon Khing (2015) Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems. Undergraduate Final Project Report thesis, Faculty of Agro Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description There are a lots of antioxidant food are thermo-labile. The effect of the decoction of Plectranthus amboinicus (P. amboinicus) stems which were rarely utilized by people had been study in this research with three difference solvents (ethanol, ethyl acetate, and water) extraction respectively. The decoction time were divided into 0, 30, 60, 90 and 120 minutes. The powdered water extracts were obtained by freeze dried of the filtrate of decoction. The remaining biomass were further extract by ethyl acetate followed by ethanol. The antioxidant activity of P.amboinicus stems was determined by DPPH (1, 1-diphenyl-2-picrylhydrazine) scavenging assay. The total phenolic content of the P.amboinicus stems were expressed as gallic acid equivalents using acid standard curve of gallic acid (R²=0.9834. The qualitative analysis of thin layer chromatography (TLC) and DPPH autobiography were used to support the quantitative analysis as the evidence. The percentage of scavenging activity showed the ethyl acetate extracted of P.amboinicus has the highest antioxidant activity with the lowest (1C50) value 32.58 ± 0.014 at 120 minutes decoction. There are no significant difference (P<0.05) between the decoction of time and the antioxidant activity. Therefore, the decoction are not deteriorate the antioxidant activity of P.amboinicus stems. The TLC-DPPH autobiography were showed similar pattern within the three difference solvents.
format Undergraduate Final Project Report
author Tan, Soon Khing
spellingShingle Tan, Soon Khing
Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems
author_facet Tan, Soon Khing
author_sort Tan, Soon Khing
title Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems
title_short Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems
title_full Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems
title_fullStr Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems
title_full_unstemmed Effect of Decoction on Antioxidant Activity of Pletranthus Amboinicus Stems
title_sort effect of decoction on antioxidant activity of pletranthus amboinicus stems
publishDate 2015
url http://discol.umk.edu.my/id/eprint/6763/
_version_ 1763303743676219392
score 13.211869

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