Cover Image

A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera)

Lipase is a triacylglycerol-hydrolyzing enzyme which is catalyzed the hydrolysis of water insoluble free fatty acids and. The whole protein extraction process was conducted at 4°C and in a sterile manner. Mesocarp of the oil palm is used to extract lipase. The presence of lipase in crude protein is...

Full description

Saved in:
Bibliographic Details
Main Author: Wong, Kien Tiek
Format: Undergraduate Final Project Report
Published: 2013
Online Access:http://discol.umk.edu.my/id/eprint/5776/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.umk.eprints.5776
record_format eprints
spelling my.umk.eprints.57762022-05-23T08:43:06Z http://discol.umk.edu.my/id/eprint/5776/ A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera) Wong, Kien Tiek Lipase is a triacylglycerol-hydrolyzing enzyme which is catalyzed the hydrolysis of water insoluble free fatty acids and. The whole protein extraction process was conducted at 4°C and in a sterile manner. Mesocarp of the oil palm is used to extract lipase. The presence of lipase in crude protein is determined by plate agar with tributyrin as substrate. Purification step was done by size-exclusion chromatography. The molecularweight of partial purified lipase was determined by SDS-PAGE. Titration with NaOH was used to conduct lipase assay and applied to find out the enzyme characterization. The optimum pH range for activity of lipase was alkaline pH,ranges, about pH 8.0 and 9.0. The optimum temperature was 40°C. In the presence of metal ions, some additives sharply decreased enzyme activity while some additives does not affect the enzyme activity. Lipase activity increase significantly in presence of CaCl₂ and MgCl₂. Low concentration of CaCl₂ increases the lipase activity but when increased to 50mM, its activity reduce sharply. Approximate molecular mass of partially purified enzyme was between 45kDa and 66.2kDa. It was found that lipase activity from over ripe oil palm is slightly higher compared ripe oil palm. 2013 Undergraduate Final Project Report NonPeerReviewed Wong, Kien Tiek (2013) A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera). Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Lipase is a triacylglycerol-hydrolyzing enzyme which is catalyzed the hydrolysis of water insoluble free fatty acids and. The whole protein extraction process was conducted at 4°C and in a sterile manner. Mesocarp of the oil palm is used to extract lipase. The presence of lipase in crude protein is determined by plate agar with tributyrin as substrate. Purification step was done by size-exclusion chromatography. The molecularweight of partial purified lipase was determined by SDS-PAGE. Titration with NaOH was used to conduct lipase assay and applied to find out the enzyme characterization. The optimum pH range for activity of lipase was alkaline pH,ranges, about pH 8.0 and 9.0. The optimum temperature was 40°C. In the presence of metal ions, some additives sharply decreased enzyme activity while some additives does not affect the enzyme activity. Lipase activity increase significantly in presence of CaCl₂ and MgCl₂. Low concentration of CaCl₂ increases the lipase activity but when increased to 50mM, its activity reduce sharply. Approximate molecular mass of partially purified enzyme was between 45kDa and 66.2kDa. It was found that lipase activity from over ripe oil palm is slightly higher compared ripe oil palm.
format Undergraduate Final Project Report
author Wong, Kien Tiek
spellingShingle Wong, Kien Tiek
A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera)
author_facet Wong, Kien Tiek
author_sort Wong, Kien Tiek
title A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera)
title_short A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera)
title_full A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera)
title_fullStr A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera)
title_full_unstemmed A comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (E. guineensis var. Tenera)
title_sort comparative studies of partial purified lipase from different maturity stages of oil palm mesocarp (e. guineensis var. tenera)
publishDate 2013
url http://discol.umk.edu.my/id/eprint/5776/
_version_ 1763303601563762688
score 13.211869

Similar Items