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The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6)

Sterilization technique is the most crucial procedure in preparing explants for in vitro propagation. It is difficult to controlling the microbial contaminants especially in woody plant. Four methods of surface sterilization by using different concentration and time exposure of different sterilizing...

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Main Author: Nur Athirah Mohd Azam
Format: Undergraduate Final Project Report
Language:English
Published: 2018
Online Access:http://discol.umk.edu.my/id/eprint/5115/1/NUR%20ATHIRAH%20BINTI%20MOHD%20AZAM.pdf
http://discol.umk.edu.my/id/eprint/5115/
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spelling my.umk.eprints.51152022-05-23T18:26:02Z http://discol.umk.edu.my/id/eprint/5115/ The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6) Nur Athirah Mohd Azam Sterilization technique is the most crucial procedure in preparing explants for in vitro propagation. It is difficult to controlling the microbial contaminants especially in woody plant. Four methods of surface sterilization by using different concentration and time exposure of different sterilizing agents; Sodium hypochlorite (NaOCl) and Hydrogen peroxide (H2O2) with the presence and absence of a fungicide treatment (2000mg/L of THIRAM 80) were conducted in this study. The main purpose of this study is to establish the sterilization method for the Ficus carica cv.BTM6 explants. BTM6 was cultured from the shoot tips and nodal segment in MS media fortified with hormones of 2.0mg/L BAP and 0.2mg/L NAA. The results obtained indicate that these treatments of sterilization methods do not give positive effects in reducing the contamination on the BTM6 in vitro propagation. Thus, further study is needed to establish in vitro propagation of BTM6. 2018 Undergraduate Final Project Report NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/5115/1/NUR%20ATHIRAH%20BINTI%20MOHD%20AZAM.pdf Nur Athirah Mohd Azam (2018) The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6). Final Year Project thesis, Universiti Malaysia Kelantan. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description Sterilization technique is the most crucial procedure in preparing explants for in vitro propagation. It is difficult to controlling the microbial contaminants especially in woody plant. Four methods of surface sterilization by using different concentration and time exposure of different sterilizing agents; Sodium hypochlorite (NaOCl) and Hydrogen peroxide (H2O2) with the presence and absence of a fungicide treatment (2000mg/L of THIRAM 80) were conducted in this study. The main purpose of this study is to establish the sterilization method for the Ficus carica cv.BTM6 explants. BTM6 was cultured from the shoot tips and nodal segment in MS media fortified with hormones of 2.0mg/L BAP and 0.2mg/L NAA. The results obtained indicate that these treatments of sterilization methods do not give positive effects in reducing the contamination on the BTM6 in vitro propagation. Thus, further study is needed to establish in vitro propagation of BTM6.
format Undergraduate Final Project Report
author Nur Athirah Mohd Azam
spellingShingle Nur Athirah Mohd Azam
The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6)
author_facet Nur Athirah Mohd Azam
author_sort Nur Athirah Mohd Azam
title The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6)
title_short The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6)
title_full The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6)
title_fullStr The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6)
title_full_unstemmed The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6)
title_sort establishment of surface sterilization for the microprogation of ficus carica cv. btm6 (brown turkey modified 6)
publishDate 2018
url http://discol.umk.edu.my/id/eprint/5115/1/NUR%20ATHIRAH%20BINTI%20MOHD%20AZAM.pdf
http://discol.umk.edu.my/id/eprint/5115/
_version_ 1763303508401979392
score 13.211869

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