The establishment of surface sterilization for the microprogation of ficus carica cv. Btm6 (brown turkey modified 6)
Sterilization technique is the most crucial procedure in preparing explants for in vitro propagation. It is difficult to controlling the microbial contaminants especially in woody plant. Four methods of surface sterilization by using different concentration and time exposure of different sterilizing...
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| Format: | Undergraduate Final Project Report |
| Language: | English |
| Published: |
2018
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| Online Access: | http://discol.umk.edu.my/id/eprint/5115/1/NUR%20ATHIRAH%20BINTI%20MOHD%20AZAM.pdf http://discol.umk.edu.my/id/eprint/5115/ |
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| Summary: | Sterilization technique is the most crucial procedure in preparing explants for in vitro propagation. It is difficult to controlling the microbial contaminants especially in woody plant. Four methods of surface sterilization by using different concentration and time exposure of different sterilizing agents; Sodium hypochlorite (NaOCl) and Hydrogen peroxide (H2O2) with the presence and absence of a fungicide treatment (2000mg/L of THIRAM 80) were conducted in this study. The main purpose of this study is to establish the sterilization method for the Ficus carica cv.BTM6 explants. BTM6 was cultured from the shoot tips and nodal segment in MS media fortified with hormones of 2.0mg/L BAP and 0.2mg/L NAA. The results obtained indicate that these treatments of sterilization methods do not give positive effects in reducing the contamination on the BTM6 in vitro propagation. Thus, further study is needed to establish in vitro propagation of BTM6. |
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