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In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram

Curcuma mangga Val., commonly known as the mango ginger has been used traditionally as a seasoning for food and as a treatment for stomach aches, fever, and cancer. Due to its medicinal importance, a systematic approach was taken to establish a rapidly growing suspension culture of C. mangga. In ini...

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Main Author: Meenakshi Sundram, Tamil Chelvan
Format: Thesis
Published: 2012
Subjects:
QH301 Biology
Online Access:http://studentsrepo.um.edu.my/3802/1/1._Title_page%2C_abstract%2C_content.pdf
http://studentsrepo.um.edu.my/3802/2/2._orginal_literacy_declaration.pdf
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spelling my.um.stud.38022013-08-30T04:19:19Z In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram Meenakshi Sundram, Tamil Chelvan QH301 Biology Curcuma mangga Val., commonly known as the mango ginger has been used traditionally as a seasoning for food and as a treatment for stomach aches, fever, and cancer. Due to its medicinal importance, a systematic approach was taken to establish a rapidly growing suspension culture of C. mangga. In initiating callus, various responses were obtained from shoot bud explants cultured on MS basal medium supplemented with different concentrations of 2,4-D, NAA and IAA either alone or in combinations. Different concentrations of sucrose were also tested. Rapid growing friable callusobtained from shoot explants cultured on MS basal medium supplemented with 1 mg l-1 2,4-D, 30 g l-1 sucrose and 2 g l-1 gelrite were selected for the initiation of suspension cultures based on histological morphology. From various medium screened, rapid growing suspension cultures were established by using MS liquid medium supplemented with 0.3 mg l-1 2,4-D, 0.1 mg l-1 NAA, 30 g l-1 sucrose, 0.1 g l-1 malt extract, 0.5 mg l-1 d-Biotin, 100 mg l-1 glutamine, 5 mg l-1 ascorbic acid and citric acid respectively. Phenolic compounds production was most effectively controlled by the incorporation of ascorbic and citric acid as antioxidants. (E)-labda-8(17),12-dien-15,16 dial is one of the bioactive compounds isolated from rhizomes of C. mangga. Recently, the cytotoxicity of this compound against cancer cells has been reported. (E)-Labda-8(17),12-dien-15,16 dial was extracted from cells, callus, rhizomes and shoots using solvent extraction method. Gas Chromatography (GC) and Gas Chromatography Flame Ionization Detector (GCFID) was used to compare the quantity of (E)-labda-8(17),12-dien-15,16 dial production in suspension cells and callus induced through various treatment and at different growth period.The presence of this compound in field grown rhizomes and in vivo shoot buds samples were also tested. The amount of (E)-labda-8(17),12-dien-15,16 dial in in vivo sources was higher than the in vitro sources, but the presence can be enhanced through various method in future studies. 2012 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/3802/1/1._Title_page%2C_abstract%2C_content.pdf application/pdf http://studentsrepo.um.edu.my/3802/2/2._orginal_literacy_declaration.pdf application/pdf http://studentsrepo.um.edu.my/3802/3/3._FULL_THESIS_final.pdf application/pdf http://studentsrepo.um.edu.my/3802/4/4._PUBLICATION%2D_APPENDIX.pdf application/pdf http://studentsrepo.um.edu.my/3802/5/FRONT_COVER_PAGE.pdf http://pendeta.um.edu.my/client/default/search/results?qu=In+vitro+cultures+of+Curcuma+mangga+Val.+for+the+production&te= Meenakshi Sundram, Tamil Chelvan (2012) In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram. Masters thesis, University of Malaya. http://studentsrepo.um.edu.my/3802/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Meenakshi Sundram, Tamil Chelvan
In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram
description Curcuma mangga Val., commonly known as the mango ginger has been used traditionally as a seasoning for food and as a treatment for stomach aches, fever, and cancer. Due to its medicinal importance, a systematic approach was taken to establish a rapidly growing suspension culture of C. mangga. In initiating callus, various responses were obtained from shoot bud explants cultured on MS basal medium supplemented with different concentrations of 2,4-D, NAA and IAA either alone or in combinations. Different concentrations of sucrose were also tested. Rapid growing friable callusobtained from shoot explants cultured on MS basal medium supplemented with 1 mg l-1 2,4-D, 30 g l-1 sucrose and 2 g l-1 gelrite were selected for the initiation of suspension cultures based on histological morphology. From various medium screened, rapid growing suspension cultures were established by using MS liquid medium supplemented with 0.3 mg l-1 2,4-D, 0.1 mg l-1 NAA, 30 g l-1 sucrose, 0.1 g l-1 malt extract, 0.5 mg l-1 d-Biotin, 100 mg l-1 glutamine, 5 mg l-1 ascorbic acid and citric acid respectively. Phenolic compounds production was most effectively controlled by the incorporation of ascorbic and citric acid as antioxidants. (E)-labda-8(17),12-dien-15,16 dial is one of the bioactive compounds isolated from rhizomes of C. mangga. Recently, the cytotoxicity of this compound against cancer cells has been reported. (E)-Labda-8(17),12-dien-15,16 dial was extracted from cells, callus, rhizomes and shoots using solvent extraction method. Gas Chromatography (GC) and Gas Chromatography Flame Ionization Detector (GCFID) was used to compare the quantity of (E)-labda-8(17),12-dien-15,16 dial production in suspension cells and callus induced through various treatment and at different growth period.The presence of this compound in field grown rhizomes and in vivo shoot buds samples were also tested. The amount of (E)-labda-8(17),12-dien-15,16 dial in in vivo sources was higher than the in vitro sources, but the presence can be enhanced through various method in future studies.
format Thesis
author Meenakshi Sundram, Tamil Chelvan
author_facet Meenakshi Sundram, Tamil Chelvan
author_sort Meenakshi Sundram, Tamil Chelvan
title In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram
title_short In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram
title_full In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram
title_fullStr In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram
title_full_unstemmed In vitro cultures of Curcuma mangga Val. for the production of (E)-labda-8(17), 12-diene-15, 16-dial / Tamil Chelvan Meenakshi Sundram
title_sort in vitro cultures of curcuma mangga val. for the production of (e)-labda-8(17), 12-diene-15, 16-dial / tamil chelvan meenakshi sundram
publishDate 2012
url http://studentsrepo.um.edu.my/3802/1/1._Title_page%2C_abstract%2C_content.pdf
http://studentsrepo.um.edu.my/3802/2/2._orginal_literacy_declaration.pdf
http://studentsrepo.um.edu.my/3802/3/3._FULL_THESIS_final.pdf
http://studentsrepo.um.edu.my/3802/4/4._PUBLICATION%2D_APPENDIX.pdf
http://studentsrepo.um.edu.my/3802/5/FRONT_COVER_PAGE.pdf
http://pendeta.um.edu.my/client/default/search/results?qu=In+vitro+cultures+of+Curcuma+mangga+Val.+for+the+production&te=
http://studentsrepo.um.edu.my/3802/
_version_ 1738505608405975040
score 13.211869

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