Cover Image

Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen

Scleractinian corals (also known as hard corals) are organisms comprised of colonies polyps that harbor assemblage of microbes, known collectively as coral holobionts which comprises of algae, bacteria, virus, fungi and archaea. Despite the prevalence of coral fungi, not much is known regarding thei...

Full description

Saved in:
Bibliographic Details
Main Author: Lee , Li Chuen
Format: Thesis
Published: 2021
Subjects:
Q Science (General)
QH301 Biology
Online Access:http://studentsrepo.um.edu.my/15092/2/Lee_Li_Chuen.pdf
http://studentsrepo.um.edu.my/15092/1/Lee_Li_Chuen.pdf
http://studentsrepo.um.edu.my/15092/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.stud.15092
record_format eprints
spelling my.um.stud.150922024-06-13T18:52:35Z Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen Lee , Li Chuen Q Science (General) QH301 Biology Scleractinian corals (also known as hard corals) are organisms comprised of colonies polyps that harbor assemblage of microbes, known collectively as coral holobionts which comprises of algae, bacteria, virus, fungi and archaea. Despite the prevalence of coral fungi, not much is known regarding their role and interaction with its host, especially their secondary metabolites aspect. The marine environment hosts fungal community with a contrasting metabolic profile as compared to its terrestrial counterparts. In current study, a total of 31 marine fungi has been isolated from eight scleractinian corals. Penicillium and Neodevriesia both frequently occurred in the collected scleractinian corals. Using multigene phylogeny approaches, selected strains were identified as Colletotrichum siamense (from Porites sp2) and Neodevriesia sp. 4 (from Galaxea astreata) for studies of their secondary metabolites. Fungal strains were cultured in potato dextrose broth for 30 days and mycelium were filtered and macerated in methanol. Methanol extract were further partitioned with ethyl acetate and water. Ethyl acetate extract were undergone further purification using column chromatography and preparative thin-layered chromatography to isolate secondary metabolites. Partially purified isolates were evaluated using NMR and methylated lipid layers were analysed for fatty acid composition. Investigated strains of fungus showed contrasting FAME profiles making it potential identification markers to fungus. Crude extracts and fractions of C. siamense were inactive against bacterial strains. 1H- NMR results showed that majority of the fatty acids isolated are variation of very-long-chained fatty acids. 2021-10 Thesis NonPeerReviewed application/pdf http://studentsrepo.um.edu.my/15092/2/Lee_Li_Chuen.pdf application/pdf http://studentsrepo.um.edu.my/15092/1/Lee_Li_Chuen.pdf Lee , Li Chuen (2021) Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen. Masters thesis, Universiti Malaya. http://studentsrepo.um.edu.my/15092/
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Student Repository
url_provider http://studentsrepo.um.edu.my/
topic Q Science (General)
QH301 Biology
spellingShingle Q Science (General)
QH301 Biology
Lee , Li Chuen
Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen
description Scleractinian corals (also known as hard corals) are organisms comprised of colonies polyps that harbor assemblage of microbes, known collectively as coral holobionts which comprises of algae, bacteria, virus, fungi and archaea. Despite the prevalence of coral fungi, not much is known regarding their role and interaction with its host, especially their secondary metabolites aspect. The marine environment hosts fungal community with a contrasting metabolic profile as compared to its terrestrial counterparts. In current study, a total of 31 marine fungi has been isolated from eight scleractinian corals. Penicillium and Neodevriesia both frequently occurred in the collected scleractinian corals. Using multigene phylogeny approaches, selected strains were identified as Colletotrichum siamense (from Porites sp2) and Neodevriesia sp. 4 (from Galaxea astreata) for studies of their secondary metabolites. Fungal strains were cultured in potato dextrose broth for 30 days and mycelium were filtered and macerated in methanol. Methanol extract were further partitioned with ethyl acetate and water. Ethyl acetate extract were undergone further purification using column chromatography and preparative thin-layered chromatography to isolate secondary metabolites. Partially purified isolates were evaluated using NMR and methylated lipid layers were analysed for fatty acid composition. Investigated strains of fungus showed contrasting FAME profiles making it potential identification markers to fungus. Crude extracts and fractions of C. siamense were inactive against bacterial strains. 1H- NMR results showed that majority of the fatty acids isolated are variation of very-long-chained fatty acids.
format Thesis
author Lee , Li Chuen
author_facet Lee , Li Chuen
author_sort Lee , Li Chuen
title Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen
title_short Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen
title_full Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen
title_fullStr Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen
title_full_unstemmed Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen
title_sort diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / lee li chuen
publishDate 2021
url http://studentsrepo.um.edu.my/15092/2/Lee_Li_Chuen.pdf
http://studentsrepo.um.edu.my/15092/1/Lee_Li_Chuen.pdf
http://studentsrepo.um.edu.my/15092/
_version_ 1805882086003834880
score 13.211869

Similar Items