Diversity of marine fungi isolated from selected scleractinian corals and their secondary metabolites / Lee Li Chuen
Scleractinian corals (also known as hard corals) are organisms comprised of colonies polyps that harbor assemblage of microbes, known collectively as coral holobionts which comprises of algae, bacteria, virus, fungi and archaea. Despite the prevalence of coral fungi, not much is known regarding thei...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Published: |
2021
|
| Subjects: | |
| Online Access: | http://studentsrepo.um.edu.my/15092/2/Lee_Li_Chuen.pdf http://studentsrepo.um.edu.my/15092/1/Lee_Li_Chuen.pdf http://studentsrepo.um.edu.my/15092/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Scleractinian corals (also known as hard corals) are organisms comprised of colonies polyps that harbor assemblage of microbes, known collectively as coral holobionts which comprises of algae, bacteria, virus, fungi and archaea. Despite the prevalence of coral fungi, not much is known regarding their role and interaction with its host, especially their secondary metabolites aspect. The marine environment hosts fungal community with a contrasting metabolic profile as compared to its terrestrial counterparts. In current study, a total of 31 marine fungi has been isolated from eight scleractinian corals. Penicillium and Neodevriesia both frequently occurred in the collected scleractinian
corals. Using multigene phylogeny approaches, selected strains were identified as
Colletotrichum siamense (from Porites sp2) and Neodevriesia sp. 4 (from Galaxea
astreata) for studies of their secondary metabolites. Fungal strains were cultured in potato
dextrose broth for 30 days and mycelium were filtered and macerated in methanol.
Methanol extract were further partitioned with ethyl acetate and water. Ethyl acetate
extract were undergone further purification using column chromatography and
preparative thin-layered chromatography to isolate secondary metabolites. Partially
purified isolates were evaluated using NMR and methylated lipid layers were analysed
for fatty acid composition. Investigated strains of fungus showed contrasting FAME
profiles making it potential identification markers to fungus. Crude extracts and fractions
of C. siamense were inactive against bacterial strains. 1H- NMR results showed that
majority of the fatty acids isolated are variation of very-long-chained fatty acids.
|
|---|