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Ammonium uptake and growth kinetics of pseudomonas putida PGA1

Ammonium uptake kinetics of low specific growth rate cultures of Pseudomonas putida PGA1, a gram negative fluorescent pseudomonad, which is an important producer of poly(3-hydroxyalkanoates) (PHA) molecules, were studied. The ammonium uptake follows a first-order kinetics model, indicating that the...

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Main Authors: Annuar, Mohamad Suffian Mohamad, Tan, Irene Kit Ping, Ibrahim, Shaliza, Ramachandran, K.B.
Format: Article
Language:English
Published: Malaysian Society for Molecular Biology and Biotechnology 2006
Subjects:
Q Science (General)
QR Microbiology
Online Access:http://eprints.um.edu.my/5373/1/Ammonium_uptake_and_growth_kinetics_of_Pseudomonas_putida_PGA1.pdf
http://eprints.um.edu.my/5373/
http://www.msmbb.my/index.php/memberships/subscription/2-uncategorised/302-abstract-ammonium-uptake-and-growth-kinetics-of-pseudomonas-putida-pga1
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spelling my.um.eprints.53732020-05-29T02:45:31Z http://eprints.um.edu.my/5373/ Ammonium uptake and growth kinetics of pseudomonas putida PGA1 Annuar, Mohamad Suffian Mohamad Tan, Irene Kit Ping Ibrahim, Shaliza Ramachandran, K.B. Q Science (General) QR Microbiology Ammonium uptake kinetics of low specific growth rate cultures of Pseudomonas putida PGA1, a gram negative fluorescent pseudomonad, which is an important producer of poly(3-hydroxyalkanoates) (PHA) molecules, were studied. The ammonium uptake follows a first-order kinetics model, indicating that the micro-organism’s specific uptake rate of ammonium and its growth would increase as the ammonium ion concentrations become higher. Optimal ammonium uptake and growth rates were observed at 0.1 gL-1 ammonium ion in aqueous medium. It also indicated that the ammonium transport mechanism is probably inducible by ammonium concentration. The limit of ammonium ion level increase before cells stop growing completely is given by the substrate inhibition constant, Ki which equals to 1.2 gL-1 ammonium ion concentration. Malaysian Society for Molecular Biology and Biotechnology 2006 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5373/1/Ammonium_uptake_and_growth_kinetics_of_Pseudomonas_putida_PGA1.pdf Annuar, Mohamad Suffian Mohamad and Tan, Irene Kit Ping and Ibrahim, Shaliza and Ramachandran, K.B. (2006) Ammonium uptake and growth kinetics of pseudomonas putida PGA1. Asia Pacific Journal of Molecular Biology and Biotechnology, 14 (1). pp. 1-10. ISSN 0128-7451 http://www.msmbb.my/index.php/memberships/subscription/2-uncategorised/302-abstract-ammonium-uptake-and-growth-kinetics-of-pseudomonas-putida-pga1
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic Q Science (General)
QR Microbiology
spellingShingle Q Science (General)
QR Microbiology
Annuar, Mohamad Suffian Mohamad
Tan, Irene Kit Ping
Ibrahim, Shaliza
Ramachandran, K.B.
Ammonium uptake and growth kinetics of pseudomonas putida PGA1
description Ammonium uptake kinetics of low specific growth rate cultures of Pseudomonas putida PGA1, a gram negative fluorescent pseudomonad, which is an important producer of poly(3-hydroxyalkanoates) (PHA) molecules, were studied. The ammonium uptake follows a first-order kinetics model, indicating that the micro-organism’s specific uptake rate of ammonium and its growth would increase as the ammonium ion concentrations become higher. Optimal ammonium uptake and growth rates were observed at 0.1 gL-1 ammonium ion in aqueous medium. It also indicated that the ammonium transport mechanism is probably inducible by ammonium concentration. The limit of ammonium ion level increase before cells stop growing completely is given by the substrate inhibition constant, Ki which equals to 1.2 gL-1 ammonium ion concentration.
format Article
author Annuar, Mohamad Suffian Mohamad
Tan, Irene Kit Ping
Ibrahim, Shaliza
Ramachandran, K.B.
author_facet Annuar, Mohamad Suffian Mohamad
Tan, Irene Kit Ping
Ibrahim, Shaliza
Ramachandran, K.B.
author_sort Annuar, Mohamad Suffian Mohamad
title Ammonium uptake and growth kinetics of pseudomonas putida PGA1
title_short Ammonium uptake and growth kinetics of pseudomonas putida PGA1
title_full Ammonium uptake and growth kinetics of pseudomonas putida PGA1
title_fullStr Ammonium uptake and growth kinetics of pseudomonas putida PGA1
title_full_unstemmed Ammonium uptake and growth kinetics of pseudomonas putida PGA1
title_sort ammonium uptake and growth kinetics of pseudomonas putida pga1
publisher Malaysian Society for Molecular Biology and Biotechnology
publishDate 2006
url http://eprints.um.edu.my/5373/1/Ammonium_uptake_and_growth_kinetics_of_Pseudomonas_putida_PGA1.pdf
http://eprints.um.edu.my/5373/
http://www.msmbb.my/index.php/memberships/subscription/2-uncategorised/302-abstract-ammonium-uptake-and-growth-kinetics-of-pseudomonas-putida-pga1
_version_ 1669007995604828160
score 13.211869

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