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The Tannase from red yeast Rhodotorula glutinis: Purification and characterization

The tannase enzyme was successfully purified to homogeneity from the culture broth of red yeast strain Rhodotorula glutinis DB2 in a three-tandem step involving ultrafiltration (5 kDa and 100 kDa systems), Sephadex G-200 gel filtration chromatography, and DEAE Sepharose CL-4B anion exchange chromato...

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Main Authors: Ong, Chong-Boon, Ibrahim, Darah, Kassim, Mohd Jain Noordin Mohd
Format: Article
Published: Taylor and Francis Ltd. 2024
Subjects:
QD Chemistry
QH301 Biology
Online Access:http://eprints.um.edu.my/46093/
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spelling my.um.eprints.460932024-08-13T03:08:21Z http://eprints.um.edu.my/46093/ The Tannase from red yeast Rhodotorula glutinis: Purification and characterization Ong, Chong-Boon Ibrahim, Darah Kassim, Mohd Jain Noordin Mohd QD Chemistry QH301 Biology The tannase enzyme was successfully purified to homogeneity from the culture broth of red yeast strain Rhodotorula glutinis DB2 in a three-tandem step involving ultrafiltration (5 kDa and 100 kDa systems), Sephadex G-200 gel filtration chromatography, and DEAE Sepharose CL-4B anion exchange chromatography. The purified tannase appeared to be homogeneous on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified tannase had a specific activity of 3.33 U mg(-1), with a 1.3% recovery and overall purification of 302-fold. The molecular mass of the tannase estimated by SDS-PAGE was about 73 kDa. The tannase had an optimum pH of 6.0 and an optimum temperature of 40 degrees C. The most stable pH was 7.0, and the enzyme was stable up to 40 degrees C. One mmol L-1 of Fe3+, Sr2+, Na+, and Pb2+ were found to promote tannase activity, whilst 1.0 mmol L-1 of Ba2+, Ca2+, Mg2+, Zn2+, Hg+, Ag+, Co2+, Fe2+, Mn2+, Cu2+, Cd-2+, Al3+, K+, Ni2+, and Li+ inhibited tannase activity. Taylor and Francis Ltd. 2024-03 Article PeerReviewed Ong, Chong-Boon and Ibrahim, Darah and Kassim, Mohd Jain Noordin Mohd (2024) The Tannase from red yeast Rhodotorula glutinis: Purification and characterization. Biocatalysis and Biotransformation, 42 (2). pp. 110-117. ISSN 1024-2422, DOI https://doi.org/10.1080/10242422.2022.2136523 <https://doi.org/10.1080/10242422.2022.2136523>. 10.1080/10242422.2022.2136523
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QD Chemistry
QH301 Biology
spellingShingle QD Chemistry
QH301 Biology
Ong, Chong-Boon
Ibrahim, Darah
Kassim, Mohd Jain Noordin Mohd
The Tannase from red yeast Rhodotorula glutinis: Purification and characterization
description The tannase enzyme was successfully purified to homogeneity from the culture broth of red yeast strain Rhodotorula glutinis DB2 in a three-tandem step involving ultrafiltration (5 kDa and 100 kDa systems), Sephadex G-200 gel filtration chromatography, and DEAE Sepharose CL-4B anion exchange chromatography. The purified tannase appeared to be homogeneous on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified tannase had a specific activity of 3.33 U mg(-1), with a 1.3% recovery and overall purification of 302-fold. The molecular mass of the tannase estimated by SDS-PAGE was about 73 kDa. The tannase had an optimum pH of 6.0 and an optimum temperature of 40 degrees C. The most stable pH was 7.0, and the enzyme was stable up to 40 degrees C. One mmol L-1 of Fe3+, Sr2+, Na+, and Pb2+ were found to promote tannase activity, whilst 1.0 mmol L-1 of Ba2+, Ca2+, Mg2+, Zn2+, Hg+, Ag+, Co2+, Fe2+, Mn2+, Cu2+, Cd-2+, Al3+, K+, Ni2+, and Li+ inhibited tannase activity.
format Article
author Ong, Chong-Boon
Ibrahim, Darah
Kassim, Mohd Jain Noordin Mohd
author_facet Ong, Chong-Boon
Ibrahim, Darah
Kassim, Mohd Jain Noordin Mohd
author_sort Ong, Chong-Boon
title The Tannase from red yeast Rhodotorula glutinis: Purification and characterization
title_short The Tannase from red yeast Rhodotorula glutinis: Purification and characterization
title_full The Tannase from red yeast Rhodotorula glutinis: Purification and characterization
title_fullStr The Tannase from red yeast Rhodotorula glutinis: Purification and characterization
title_full_unstemmed The Tannase from red yeast Rhodotorula glutinis: Purification and characterization
title_sort tannase from red yeast rhodotorula glutinis: purification and characterization
publisher Taylor and Francis Ltd.
publishDate 2024
url http://eprints.um.edu.my/46093/
_version_ 1809136926670716928
score 13.211869

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