Staff View: Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes

Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes

Thioredoxin interacting protein (TXNIP) has emerged as a significant regulator of 8-cell mass and loss, rendering it an attractive target for treating diabetes. We previously showed that Shiga-Y6, a fluorinated curcumin derivative, inhibited TXNIP mRNA and protein expression in vitro, raising the qu...

Full description

Saved in:

Bibliographic Details
Main Authors:	Aldoghachi, Asraa Faris, Yanagisawa, Daijiro, Arrozi, Aslina Pahrudin, Bakar, Zulzikry Hafiz Abu, Taguchi, Hiroyasu, Ishigaki, Shinsuke, Morino, Katsutaro, Tooyama, Ikuo
Format:	Article
Published:	ACADEMIC PRESS INC ELSEVIER SCIENCE 2024
Subjects:	R Medicine (General) RM Therapeutics. Pharmacology
Online Access:	http://eprints.um.edu.my/44220/ https://doi.org/10.1016/j.bbrc.2023.149392
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.um.eprints.44220
record_format	eprints
spelling	my.um.eprints.442202024-06-25T04:53:15Z http://eprints.um.edu.my/44220/ Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes Aldoghachi, Asraa Faris Yanagisawa, Daijiro Arrozi, Aslina Pahrudin Bakar, Zulzikry Hafiz Abu Taguchi, Hiroyasu Ishigaki, Shinsuke Morino, Katsutaro Tooyama, Ikuo R Medicine (General) RM Therapeutics. Pharmacology Thioredoxin interacting protein (TXNIP) has emerged as a significant regulator of 8-cell mass and loss, rendering it an attractive target for treating diabetes. We previously showed that Shiga-Y6, a fluorinated curcumin derivative, inhibited TXNIP mRNA and protein expression in vitro, raising the question of whether the same effect could be translated in vivo. Herein, we examined the effect of Shiga-Y6 on TNXIP levels and explored its therapeutic potential in a mouse model of diabetes, Akita mice. We intraperitoneally injected Shiga-Y6 (SY6; 30 mg/ kg of body weight) or vehicle into 8-week-old Akita mice for 28 consecutive days. On day 29, the mice were euthanized, following which the serum levels of glucose, insulin, and glucagon were measured using ELISA, the expression of TXNIP in pancreatic tissue lysates was determined using western blotting, and the level of 8-cell apoptosis was assessed using the TUNEL assay. TXNIP levels in the pancreatic tissue of Akita mice were significantly elevated compared with wild-type (WT) mice. Shiga-Y6 administration for 28 days significantly lowered those levels compared with Akita mice that received vehicle to a level comparable to WT mice. In immunohistochemical analysis, both a- to 8-cell ratio and the number of apoptotic 8-cells were significantly reduced in SY6-treated Akita mice, compared with vehicle-treated Akita mice. Findings from the present study suggest a potential of Shiga-Y6 as an antidiabetic agent through lowering TXNIP protein levels and ameliorating pancreatic 8-cells apoptosis. ACADEMIC PRESS INC ELSEVIER SCIENCE 2024-01-29 Article PeerReviewed Aldoghachi, Asraa Faris and Yanagisawa, Daijiro and Arrozi, Aslina Pahrudin and Bakar, Zulzikry Hafiz Abu and Taguchi, Hiroyasu and Ishigaki, Shinsuke and Morino, Katsutaro and Tooyama, Ikuo (2024) Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes. Biochemical and Biophysical Research Communications, 694. ISSN 1090-2104, DOI https://doi.org/10.1016/j.bbrc.2023.149392 <https://doi.org/10.1016/j.bbrc.2023.149392>. https://doi.org/10.1016/j.bbrc.2023.149392 10.1016/j.bbrc.2023.149392
institution	Universiti Malaya
building	UM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaya
content_source	UM Research Repository
url_provider	http://eprints.um.edu.my/
topic	R Medicine (General) RM Therapeutics. Pharmacology
spellingShingle	R Medicine (General) RM Therapeutics. Pharmacology Aldoghachi, Asraa Faris Yanagisawa, Daijiro Arrozi, Aslina Pahrudin Bakar, Zulzikry Hafiz Abu Taguchi, Hiroyasu Ishigaki, Shinsuke Morino, Katsutaro Tooyama, Ikuo Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes
description	Thioredoxin interacting protein (TXNIP) has emerged as a significant regulator of 8-cell mass and loss, rendering it an attractive target for treating diabetes. We previously showed that Shiga-Y6, a fluorinated curcumin derivative, inhibited TXNIP mRNA and protein expression in vitro, raising the question of whether the same effect could be translated in vivo. Herein, we examined the effect of Shiga-Y6 on TNXIP levels and explored its therapeutic potential in a mouse model of diabetes, Akita mice. We intraperitoneally injected Shiga-Y6 (SY6; 30 mg/ kg of body weight) or vehicle into 8-week-old Akita mice for 28 consecutive days. On day 29, the mice were euthanized, following which the serum levels of glucose, insulin, and glucagon were measured using ELISA, the expression of TXNIP in pancreatic tissue lysates was determined using western blotting, and the level of 8-cell apoptosis was assessed using the TUNEL assay. TXNIP levels in the pancreatic tissue of Akita mice were significantly elevated compared with wild-type (WT) mice. Shiga-Y6 administration for 28 days significantly lowered those levels compared with Akita mice that received vehicle to a level comparable to WT mice. In immunohistochemical analysis, both a- to 8-cell ratio and the number of apoptotic 8-cells were significantly reduced in SY6-treated Akita mice, compared with vehicle-treated Akita mice. Findings from the present study suggest a potential of Shiga-Y6 as an antidiabetic agent through lowering TXNIP protein levels and ameliorating pancreatic 8-cells apoptosis.
format	Article
author	Aldoghachi, Asraa Faris Yanagisawa, Daijiro Arrozi, Aslina Pahrudin Bakar, Zulzikry Hafiz Abu Taguchi, Hiroyasu Ishigaki, Shinsuke Morino, Katsutaro Tooyama, Ikuo
author_facet	Aldoghachi, Asraa Faris Yanagisawa, Daijiro Arrozi, Aslina Pahrudin Bakar, Zulzikry Hafiz Abu Taguchi, Hiroyasu Ishigaki, Shinsuke Morino, Katsutaro Tooyama, Ikuo
author_sort	Aldoghachi, Asraa Faris
title	Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes
title_short	Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes
title_full	Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes
title_fullStr	Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes
title_full_unstemmed	Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes
title_sort	fluorinated curcumin derivative (shiga-y6) modulates the level of thioredoxin-interacting protein (txnip) in a mouse model of diabetes
publisher	ACADEMIC PRESS INC ELSEVIER SCIENCE
publishDate	2024
url	http://eprints.um.edu.my/44220/ https://doi.org/10.1016/j.bbrc.2023.149392
_version_	1805881137007951872
score	13.211869

Fluorinated curcumin derivative (Shiga-Y6) modulates the level of thioredoxin-interacting protein (TXNIP) in a mouse model of diabetes

Similar Items