Cover Image

Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes

Purpose: The aim of this study was to evaluate the protective effect of conditioned medium derived from human adipose mesenchymal stem cells (CM-hADSCs) on C28I2 chondrocytes against oxidative stress and mitochondrial apoptosis induced by high glucose (HG). Methods: C28I2 cells were pre-treated with...

Full description

Saved in:
Bibliographic Details
Main Authors: Safari, Sedigheh, Eidi, Akram, Mehrabani, Mehrnaz, Fatemi, Mohammad Javad, Sharifi, Ali Mohammad
Format: Article
Published: Tabriz University of Medical Sciences 2022
Subjects:
R Medicine
RS Pharmacy and materia medica
Online Access:http://eprints.um.edu.my/43903/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.43903
record_format eprints
spelling my.um.eprints.439032024-06-26T01:37:50Z http://eprints.um.edu.my/43903/ Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes Safari, Sedigheh Eidi, Akram Mehrabani, Mehrnaz Fatemi, Mohammad Javad Sharifi, Ali Mohammad R Medicine RS Pharmacy and materia medica Purpose: The aim of this study was to evaluate the protective effect of conditioned medium derived from human adipose mesenchymal stem cells (CM-hADSCs) on C28I2 chondrocytes against oxidative stress and mitochondrial apoptosis induced by high glucose (HG). Methods: C28I2 cells were pre-treated with CM-hADSCs for 24 hours followed by HG exposure (75 mM) for 48 hours. MTT assay was used to assess the cell viability. Reactive oxygen species (ROS) and lipid peroxidation were determined by 2,7-dichlorofluorescein diacetate (DCFH-DA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Expressions of glutathione peroxidase 3 (GPX 3), heme oxygenase-1 (HO-1), and NAD(P)H quinone dehydrogenase 1 (NQO1) were analyzed by RT-PCR. Finally, western blot analysis was used to measure Bax, Bcl-2, cleaved caspase-3, and Nrf-2 expression at protein levels. Results: CM-hADSCs pretreatment mitigated the cytotoxic effect of HG on C28I2 viability. Treatment also markedly reduced the levels of ROS, lipid peroxidation, and augmented the expression of HO-1, NQO1, and GPx3 genes in HG-exposed group. CM-ADSCs enhanced Nrf-2 protein expression and reduced mitochondrial apoptosis through reducing Bax/Bcl-2 ratio and Caspase-3 activation. Conclusion: MSCs, probably through its paracrine effects, declined the deleterious effect of HG on chondrocytes. Hence, therapies based on MSCs secretomes appear to be a promising therapeutic approaches to prevent joint complications in diabetic patients. © 2022 The Author (s). Tabriz University of Medical Sciences 2022 Article PeerReviewed Safari, Sedigheh and Eidi, Akram and Mehrabani, Mehrnaz and Fatemi, Mohammad Javad and Sharifi, Ali Mohammad (2022) Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes. Advanced Pharmaceutical Bulletin, 12 (3). 632 – 640. ISSN 2228-5881, DOI https://doi.org/10.34172/apb.2022.044 <https://doi.org/10.34172/apb.2022.044>. 10.34172/apb.2022.044
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic R Medicine
RS Pharmacy and materia medica
spellingShingle R Medicine
RS Pharmacy and materia medica
Safari, Sedigheh
Eidi, Akram
Mehrabani, Mehrnaz
Fatemi, Mohammad Javad
Sharifi, Ali Mohammad
Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes
description Purpose: The aim of this study was to evaluate the protective effect of conditioned medium derived from human adipose mesenchymal stem cells (CM-hADSCs) on C28I2 chondrocytes against oxidative stress and mitochondrial apoptosis induced by high glucose (HG). Methods: C28I2 cells were pre-treated with CM-hADSCs for 24 hours followed by HG exposure (75 mM) for 48 hours. MTT assay was used to assess the cell viability. Reactive oxygen species (ROS) and lipid peroxidation were determined by 2,7-dichlorofluorescein diacetate (DCFH-DA) and thiobarbituric acid reactive substances (TBARS) assays, respectively. Expressions of glutathione peroxidase 3 (GPX 3), heme oxygenase-1 (HO-1), and NAD(P)H quinone dehydrogenase 1 (NQO1) were analyzed by RT-PCR. Finally, western blot analysis was used to measure Bax, Bcl-2, cleaved caspase-3, and Nrf-2 expression at protein levels. Results: CM-hADSCs pretreatment mitigated the cytotoxic effect of HG on C28I2 viability. Treatment also markedly reduced the levels of ROS, lipid peroxidation, and augmented the expression of HO-1, NQO1, and GPx3 genes in HG-exposed group. CM-ADSCs enhanced Nrf-2 protein expression and reduced mitochondrial apoptosis through reducing Bax/Bcl-2 ratio and Caspase-3 activation. Conclusion: MSCs, probably through its paracrine effects, declined the deleterious effect of HG on chondrocytes. Hence, therapies based on MSCs secretomes appear to be a promising therapeutic approaches to prevent joint complications in diabetic patients. © 2022 The Author (s).
format Article
author Safari, Sedigheh
Eidi, Akram
Mehrabani, Mehrnaz
Fatemi, Mohammad Javad
Sharifi, Ali Mohammad
author_facet Safari, Sedigheh
Eidi, Akram
Mehrabani, Mehrnaz
Fatemi, Mohammad Javad
Sharifi, Ali Mohammad
author_sort Safari, Sedigheh
title Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes
title_short Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes
title_full Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes
title_fullStr Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes
title_full_unstemmed Conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured C28I2 chondrocytes
title_sort conditioned medium of adipose-derived mesenchymal stem cells as a promising candidate to protect high glucose-induced injury in cultured c28i2 chondrocytes
publisher Tabriz University of Medical Sciences
publishDate 2022
url http://eprints.um.edu.my/43903/
_version_ 1805881124023435264
score 13.211869

Similar Items