A bioanalytical method for quantification of telmisartan in rat plasma; development, validation and application to pharmacokinetic study
The telmisartan was determined in a rat plasma using developed and validated a reversed-phase high performance liquid chromatographic (HPLC). The pre-treatment of the plasma sample involving liquid-liquid extraction using ethanol as the extracting solvent. The HPLC method validation has been shown a...
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| Main Authors: | , , , |
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| Format: | Article |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/35473/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108295714&doi=10.52711%2f0974-360X.2021.00379&partnerID=40&md5=034635993c3af6155b22906ec0c00b34 |
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| Summary: | The telmisartan was determined in a rat plasma using developed and validated a reversed-phase high performance liquid chromatographic (HPLC). The pre-treatment of the plasma sample involving liquid-liquid extraction using ethanol as the extracting solvent. The HPLC method validation has been shown a linear calibration curve over a plasma concentrations range of 0.7 to 10µg/mL with a correlation coefficient of 0.9979, the limit of detection and the limit of quantification were determined to be 0.025µg/ml and 0.07µg/ml, respectively. The precision and accuracy were in an acceptable limit. The pharmacokinetic parameters of telmisartan were adequately evaluated following a single oral dose (4mg/kg) in Sprague-Dawley rats. The results observed conclude that the developed bioanalytical HPLC method is appropriate and applicable as an analytical tool in the pharmacokinetic study of telmisartan. © RJPT All right reserved. |
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