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Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract

Multi-walled carbon nanotubes (MWCNT)-tannase composite was investigated as an immobilized biocatalyst on the basis of its facile preparation, low cost, and excellent aqueous dispersibility. Cross-linked tannase enzymes, obtained in the presence of glutaraldehyde, were composited with MWCNT via phys...

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Main Authors: Ong, Chong-Boon, Annuar, Mohamad Suffian Mohamad
Format: Article
Published: Wiley 2021
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/26921/
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spelling my.um.eprints.269212022-04-12T00:47:34Z http://eprints.um.edu.my/26921/ Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract Ong, Chong-Boon Annuar, Mohamad Suffian Mohamad QH301 Biology Multi-walled carbon nanotubes (MWCNT)-tannase composite was investigated as an immobilized biocatalyst on the basis of its facile preparation, low cost, and excellent aqueous dispersibility. Cross-linked tannase enzymes, obtained in the presence of glutaraldehyde, were composited with MWCNT via physical adsorption. Multiple techniques were applied to investigate, and corroborate the successful adsorption of cross-linked tannase onto the MWCNT structure. Green tea infusion extract post-treatment using the composite preparation showed elevated radical scavenging activities relative to the control. Green tea infusion extract exhibited a markedly reduced EC50 value on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals following its treatment with the enzyme composite, which represents 20%-34% enhancement in its free radical scavenging capacity. Stoichiometry and number of reduced DPPH were determined and compared. The antioxidative potential of a widely consumed, health-beneficial green tea is elevated by the treatment with MWCNT-tannase composite. Practical applications Cross-linked tannase enzymes were composited with pristine multi-walled carbon nanotubes via simple physical adsorption. The composite presents key advantages such as low specific volume compared to other well-known immobilization media, inert, facile enzyme composition, and ease of recovery for repeated use. The work demonstrated carbon nanotube prosthetic utility in the biotransformation of food-based health commodity sought after for its nutritional benefits. The approach is of both industrial- and agricultural importance, and is a promising and viable strategy to obtain a natural, functional food supplement for the multi-billion dollar well-being and health-related industries. Wiley 2021-10 Article PeerReviewed Ong, Chong-Boon and Annuar, Mohamad Suffian Mohamad (2021) Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract. Journal of Food Biochemistry, 45 (10). ISSN 0145-8884, DOI https://doi.org/10.1111/jfbc.13924 <https://doi.org/10.1111/jfbc.13924>. 10.1111/jfbc.13924
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Ong, Chong-Boon
Annuar, Mohamad Suffian Mohamad
Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract
description Multi-walled carbon nanotubes (MWCNT)-tannase composite was investigated as an immobilized biocatalyst on the basis of its facile preparation, low cost, and excellent aqueous dispersibility. Cross-linked tannase enzymes, obtained in the presence of glutaraldehyde, were composited with MWCNT via physical adsorption. Multiple techniques were applied to investigate, and corroborate the successful adsorption of cross-linked tannase onto the MWCNT structure. Green tea infusion extract post-treatment using the composite preparation showed elevated radical scavenging activities relative to the control. Green tea infusion extract exhibited a markedly reduced EC50 value on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals following its treatment with the enzyme composite, which represents 20%-34% enhancement in its free radical scavenging capacity. Stoichiometry and number of reduced DPPH were determined and compared. The antioxidative potential of a widely consumed, health-beneficial green tea is elevated by the treatment with MWCNT-tannase composite. Practical applications Cross-linked tannase enzymes were composited with pristine multi-walled carbon nanotubes via simple physical adsorption. The composite presents key advantages such as low specific volume compared to other well-known immobilization media, inert, facile enzyme composition, and ease of recovery for repeated use. The work demonstrated carbon nanotube prosthetic utility in the biotransformation of food-based health commodity sought after for its nutritional benefits. The approach is of both industrial- and agricultural importance, and is a promising and viable strategy to obtain a natural, functional food supplement for the multi-billion dollar well-being and health-related industries.
format Article
author Ong, Chong-Boon
Annuar, Mohamad Suffian Mohamad
author_facet Ong, Chong-Boon
Annuar, Mohamad Suffian Mohamad
author_sort Ong, Chong-Boon
title Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract
title_short Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract
title_full Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract
title_fullStr Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract
title_full_unstemmed Cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract
title_sort cross-linked tannase-carbon nanotubes composite in elevating antioxidative potential of green tea extract
publisher Wiley
publishDate 2021
url http://eprints.um.edu.my/26921/
_version_ 1735409476147609600
score 13.211869

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