Cover Image

Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update

The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began as a cluster of pneumonia cases in Wuhan, China before spreading to over 200 countries and territories on six continents in less than six months. Despite rigorous global containment a...

Full description

Saved in:
Bibliographic Details
Main Authors: Yu, Choo Yee, Chan, Kok Gan, Yean, Chan Yean, Ang, Geik Yong
Format: Article
Published: MDPI 2021
Subjects:
QH301 Biology
Online Access:http://eprints.um.edu.my/26602/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.um.eprints.26602
record_format eprints
spelling my.um.eprints.266022022-03-28T04:03:47Z http://eprints.um.edu.my/26602/ Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update Yu, Choo Yee Chan, Kok Gan Yean, Chan Yean Ang, Geik Yong QH301 Biology The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began as a cluster of pneumonia cases in Wuhan, China before spreading to over 200 countries and territories on six continents in less than six months. Despite rigorous global containment and quarantine efforts to limit the transmission of the virus, COVID-19 cases and deaths have continued to increase, leaving devastating impacts on the lives of many with far-reaching effects on the global society, economy and healthcare system. With over 43 million cases and 1.1 million deaths recorded worldwide, accurate and rapid diagnosis continues to be a cornerstone of pandemic control. In this review, we aim to present an objective overview of the latest nucleic acid-based diagnostic tests for the detection of SARS-CoV-2 that have been authorized by the Food and Drug Administration (FDA) under emergency use authorization (EUA) as of 31 October 2020. We systematically summarize and compare the principles, technologies, protocols and performance characteristics of amplification- and sequencing-based tests that have become alternatives to the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. We highlight the notable features of the tests including authorized settings, along with the advantages and disadvantages of the tests. We conclude with a brief discussion on the current challenges and future perspectives of COVID-19 diagnostics. MDPI 2021-01 Article PeerReviewed Yu, Choo Yee and Chan, Kok Gan and Yean, Chan Yean and Ang, Geik Yong (2021) Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update. Diagnostics, 11 (1). ISSN 2075-4418, DOI https://doi.org/10.3390/diagnostics11010053 <https://doi.org/10.3390/diagnostics11010053>. 10.3390/diagnostics11010053
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic QH301 Biology
spellingShingle QH301 Biology
Yu, Choo Yee
Chan, Kok Gan
Yean, Chan Yean
Ang, Geik Yong
Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update
description The coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) began as a cluster of pneumonia cases in Wuhan, China before spreading to over 200 countries and territories on six continents in less than six months. Despite rigorous global containment and quarantine efforts to limit the transmission of the virus, COVID-19 cases and deaths have continued to increase, leaving devastating impacts on the lives of many with far-reaching effects on the global society, economy and healthcare system. With over 43 million cases and 1.1 million deaths recorded worldwide, accurate and rapid diagnosis continues to be a cornerstone of pandemic control. In this review, we aim to present an objective overview of the latest nucleic acid-based diagnostic tests for the detection of SARS-CoV-2 that have been authorized by the Food and Drug Administration (FDA) under emergency use authorization (EUA) as of 31 October 2020. We systematically summarize and compare the principles, technologies, protocols and performance characteristics of amplification- and sequencing-based tests that have become alternatives to the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. We highlight the notable features of the tests including authorized settings, along with the advantages and disadvantages of the tests. We conclude with a brief discussion on the current challenges and future perspectives of COVID-19 diagnostics.
format Article
author Yu, Choo Yee
Chan, Kok Gan
Yean, Chan Yean
Ang, Geik Yong
author_facet Yu, Choo Yee
Chan, Kok Gan
Yean, Chan Yean
Ang, Geik Yong
author_sort Yu, Choo Yee
title Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update
title_short Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update
title_full Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update
title_fullStr Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update
title_full_unstemmed Nucleic acid-based diagnostic tests for the detection SARS-CoV-2: An update
title_sort nucleic acid-based diagnostic tests for the detection sars-cov-2: an update
publisher MDPI
publishDate 2021
url http://eprints.um.edu.my/26602/
_version_ 1735409433766264832
score 13.211869

Similar Items