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Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study

Background: Interleukin-10 (IL10) genotypes have been closely correlated to the susceptibility for oral squamous cell carcinoma. More than half of oral cancers in the world occur in Asia with estimated 168,850 new cases were diagnosed in this geographical region alone. Considering the rising numbers...

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Main Authors: Goud, E V Soma Sekhar, Malleedi, Shanthi, Ramanathan, Anand, Wong, Gou Rean, Hwei Ern, Beatrix Tan, Yean, Goh Yin, Ann, Ho Hui, Syan, Tan Yuen, Zain, Rosnah
Format: Article
Published: West Asia Organization for Cancer Prevention 2019
Subjects:
RK Dentistry
Online Access:http://eprints.um.edu.my/23404/
https://doi.org/10.31557/APJCP.2019.20.3.935
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spelling my.um.eprints.234042020-01-14T01:35:34Z http://eprints.um.edu.my/23404/ Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study Goud, E V Soma Sekhar Malleedi, Shanthi Ramanathan, Anand Wong, Gou Rean Hwei Ern, Beatrix Tan Yean, Goh Yin Ann, Ho Hui Syan, Tan Yuen Zain, Rosnah RK Dentistry Background: Interleukin-10 (IL10) genotypes have been closely correlated to the susceptibility for oral squamous cell carcinoma. More than half of oral cancers in the world occur in Asia with estimated 168,850 new cases were diagnosed in this geographical region alone. Considering the rising numbers of oral cancer cases in Malaysia, association of IL10 A1082G gene polymorphism was correlated. Methodology: 41 oral squamous cell carcinoma (OSCC) cases and 48 healthy controls of comparable age, gender, and with habits like smoking, alcohol consumption and betel quid chewing were selected. In this case-control study, samples were collected from the Oral Cancer Research and Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Malaysia. Genotyping conditions were evaluated by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The PCR products were subjected to digestion by MnlI enzyme (NEB, UK) to screen for the IL10 A-1082G. Digested DNA products were analyzed by electrophoresis on 4% (w/v) agarose gel, stained with ethidium bromide and imaged under UV illumination. Chi-square test and Fisher's Exact test were used in statistical analysis. Results: AG genotypes were present in 81.3% and 86.0% of healthy control and OSCC cases respectively (OR=0.468, 95% CI=0.133-1.653). No significant association was found between IL10 A1082G polymorphism with risk habits, clinico-pathological parameters and 5-years overall survival. The findings also show no significant correlation between the IL10 genotype and features of OSCC within the case group as measured by tumor size, lymph node involvement, stage, invasive front, grading, depth, pattern of invasion. Conclusion: This study suggests that functional polymorphism AG of IL10 A1082G may have no influence with OSCC susceptibility. However, further investigation with larger sample sizes can be conducted to provide additional evidence to support the lack of association of IL10 A1082G polymorphism in oral cancer. © 2019 IL10 Gene Polymorphism in Oral Cancer. West Asia Organization for Cancer Prevention 2019 Article PeerReviewed Goud, E V Soma Sekhar and Malleedi, Shanthi and Ramanathan, Anand and Wong, Gou Rean and Hwei Ern, Beatrix Tan and Yean, Goh Yin and Ann, Ho Hui and Syan, Tan Yuen and Zain, Rosnah (2019) Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study. Asian Pacific Journal of Cancer Prevention, 20 (3). pp. 935-941. ISSN 1513-7368 https://doi.org/10.31557/APJCP.2019.20.3.935 doi:10.31557/APJCP.2019.20.3.935
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic RK Dentistry
spellingShingle RK Dentistry
Goud, E V Soma Sekhar
Malleedi, Shanthi
Ramanathan, Anand
Wong, Gou Rean
Hwei Ern, Beatrix Tan
Yean, Goh Yin
Ann, Ho Hui
Syan, Tan Yuen
Zain, Rosnah
Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study
description Background: Interleukin-10 (IL10) genotypes have been closely correlated to the susceptibility for oral squamous cell carcinoma. More than half of oral cancers in the world occur in Asia with estimated 168,850 new cases were diagnosed in this geographical region alone. Considering the rising numbers of oral cancer cases in Malaysia, association of IL10 A1082G gene polymorphism was correlated. Methodology: 41 oral squamous cell carcinoma (OSCC) cases and 48 healthy controls of comparable age, gender, and with habits like smoking, alcohol consumption and betel quid chewing were selected. In this case-control study, samples were collected from the Oral Cancer Research and Coordinating Centre (OCRCC), Faculty of Dentistry, University of Malaya, Malaysia. Genotyping conditions were evaluated by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The PCR products were subjected to digestion by MnlI enzyme (NEB, UK) to screen for the IL10 A-1082G. Digested DNA products were analyzed by electrophoresis on 4% (w/v) agarose gel, stained with ethidium bromide and imaged under UV illumination. Chi-square test and Fisher's Exact test were used in statistical analysis. Results: AG genotypes were present in 81.3% and 86.0% of healthy control and OSCC cases respectively (OR=0.468, 95% CI=0.133-1.653). No significant association was found between IL10 A1082G polymorphism with risk habits, clinico-pathological parameters and 5-years overall survival. The findings also show no significant correlation between the IL10 genotype and features of OSCC within the case group as measured by tumor size, lymph node involvement, stage, invasive front, grading, depth, pattern of invasion. Conclusion: This study suggests that functional polymorphism AG of IL10 A1082G may have no influence with OSCC susceptibility. However, further investigation with larger sample sizes can be conducted to provide additional evidence to support the lack of association of IL10 A1082G polymorphism in oral cancer. © 2019 IL10 Gene Polymorphism in Oral Cancer.
format Article
author Goud, E V Soma Sekhar
Malleedi, Shanthi
Ramanathan, Anand
Wong, Gou Rean
Hwei Ern, Beatrix Tan
Yean, Goh Yin
Ann, Ho Hui
Syan, Tan Yuen
Zain, Rosnah
author_facet Goud, E V Soma Sekhar
Malleedi, Shanthi
Ramanathan, Anand
Wong, Gou Rean
Hwei Ern, Beatrix Tan
Yean, Goh Yin
Ann, Ho Hui
Syan, Tan Yuen
Zain, Rosnah
author_sort Goud, E V Soma Sekhar
title Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study
title_short Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study
title_full Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study
title_fullStr Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study
title_full_unstemmed Association of Interleukin-10 Genotypes and Oral Cancer Susceptibility in Selected Malaysian Population: A Case- Control Study
title_sort association of interleukin-10 genotypes and oral cancer susceptibility in selected malaysian population: a case- control study
publisher West Asia Organization for Cancer Prevention
publishDate 2019
url http://eprints.um.edu.my/23404/
https://doi.org/10.31557/APJCP.2019.20.3.935
_version_ 1657488204385746944
score 13.211869

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