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Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process

The production of value-added products such as cellulase and ethanol via a consolidated bioprocess could be realized by tapping into the multiple actions of a microbial community. For this purpose, an in situ saccharification and fermentation process through a sequential co-culture white-rot fungus...

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Main Authors: Yoon, Li Wan, Ngoh, Gek Cheng, Chua, Adeline Seak May, Abdul Patah, Muhamad Fazly, Teoh, Wen Hui
Format: Article
Published: Elsevier 2019
Subjects:
TP Chemical technology
Online Access:http://eprints.um.edu.my/22958/
https://doi.org/10.1016/j.cep.2019.107528
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spelling my.um.eprints.229582019-11-04T08:38:18Z http://eprints.um.edu.my/22958/ Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process Yoon, Li Wan Ngoh, Gek Cheng Chua, Adeline Seak May Abdul Patah, Muhamad Fazly Teoh, Wen Hui TP Chemical technology The production of value-added products such as cellulase and ethanol via a consolidated bioprocess could be realized by tapping into the multiple actions of a microbial community. For this purpose, an in situ saccharification and fermentation process through a sequential co-culture white-rot fungus and Saccharomyces cerevisiae on NaOH-pretreated sugarcane bagasse (SCB) was investigated. In the present work, white rot fungus plays a role in the production of cellulase enzymes. With the produced cellulase, an in situ saccharification process took place in the reactor to depolymerize pretreated SCB into reducing sugar. The reducing sugar was converted into ethanol via fermentation by S. cerevisiae, which was added into the system sequentially. White rot fungus Pycnoporus sanguineus was selected due to its competency in producing cellulase and reducing sugar production. The operating condition to maximize the production of reducing sugar in situ was obtained through a Central Composite Design method. A total of 3.13 g reducing sugar/100 g SCB was obtained when P. sanguineus was cultivated at 0.6% inoculum loading, 70% moisture content and 4 days. Subsequently, 4.5 g ethanol/100 g SCB was obtained from the in situ saccharification and fermentation system after S. cerevisiae was sequentially inoculated. Elsevier 2019 Article PeerReviewed Yoon, Li Wan and Ngoh, Gek Cheng and Chua, Adeline Seak May and Abdul Patah, Muhamad Fazly and Teoh, Wen Hui (2019) Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process. Chemical Engineering and Processing - Process Intensification, 142. p. 107528. ISSN 0255-2701 https://doi.org/10.1016/j.cep.2019.107528 doi:10.1016/j.cep.2019.107528
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Yoon, Li Wan
Ngoh, Gek Cheng
Chua, Adeline Seak May
Abdul Patah, Muhamad Fazly
Teoh, Wen Hui
Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process
description The production of value-added products such as cellulase and ethanol via a consolidated bioprocess could be realized by tapping into the multiple actions of a microbial community. For this purpose, an in situ saccharification and fermentation process through a sequential co-culture white-rot fungus and Saccharomyces cerevisiae on NaOH-pretreated sugarcane bagasse (SCB) was investigated. In the present work, white rot fungus plays a role in the production of cellulase enzymes. With the produced cellulase, an in situ saccharification process took place in the reactor to depolymerize pretreated SCB into reducing sugar. The reducing sugar was converted into ethanol via fermentation by S. cerevisiae, which was added into the system sequentially. White rot fungus Pycnoporus sanguineus was selected due to its competency in producing cellulase and reducing sugar production. The operating condition to maximize the production of reducing sugar in situ was obtained through a Central Composite Design method. A total of 3.13 g reducing sugar/100 g SCB was obtained when P. sanguineus was cultivated at 0.6% inoculum loading, 70% moisture content and 4 days. Subsequently, 4.5 g ethanol/100 g SCB was obtained from the in situ saccharification and fermentation system after S. cerevisiae was sequentially inoculated.
format Article
author Yoon, Li Wan
Ngoh, Gek Cheng
Chua, Adeline Seak May
Abdul Patah, Muhamad Fazly
Teoh, Wen Hui
author_facet Yoon, Li Wan
Ngoh, Gek Cheng
Chua, Adeline Seak May
Abdul Patah, Muhamad Fazly
Teoh, Wen Hui
author_sort Yoon, Li Wan
title Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process
title_short Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process
title_full Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process
title_fullStr Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process
title_full_unstemmed Process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process
title_sort process intensification of cellulase and bioethanol production from sugarcane bagasse via an integrated saccharification and fermentation process
publisher Elsevier
publishDate 2019
url http://eprints.um.edu.my/22958/
https://doi.org/10.1016/j.cep.2019.107528
_version_ 1651867381870362624
score 13.211869

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