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Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields

Pulse electromagnetic fields (PEMFs) have been shown to recruit calcium-signaling cascades common to chondrogenesis. Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. MSCs undergoing chondrogenesis are preferentially responsive...

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Main Authors: Parate, D., Franco-Obregón, A., Fröhlich, J., Beyer, C., Abbas, A.A., Kamarul, Tunku, Hui, J.H.P., Yang, Z.
Format: Article
Language:English
Published: Nature Publishing Group 2017
Subjects:
R Medicine
Online Access:http://eprints.um.edu.my/19065/1/Enhancement_of_mesenchymal_stem_cell_chondrogenesis_with_short-term_low_intensity_pulsed_electromagnetic_fields.pdf
http://eprints.um.edu.my/19065/
http://dx.doi.org/10.1038/s41598-017-09892-w
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spelling my.um.eprints.190652018-10-10T09:12:00Z http://eprints.um.edu.my/19065/ Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields Parate, D. Franco-Obregón, A. Fröhlich, J. Beyer, C. Abbas, A.A. Kamarul, Tunku Hui, J.H.P. Yang, Z. R Medicine Pulse electromagnetic fields (PEMFs) have been shown to recruit calcium-signaling cascades common to chondrogenesis. Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. MSCs undergoing chondrogenesis are preferentially responsive to an electromagnetic efficacy window defined by field amplitude, duration and frequency of exposure. Contrary to conventional practice of administering prolonged and repetitive exposures to PEMFs, optimal chondrogenic outcome is achieved in response to brief (10 minutes), low intensity (2 mT) exposure to 6 ms bursts of magnetic pulses, at 15 Hz, administered only once at the onset of chondrogenic induction. By contrast, repeated exposures diminished chondrogenic outcome and could be attributed to calcium entry after the initial induction. Transient receptor potential (TRP) channels appear to mediate these aspects of PEMF stimulation, serving as a conduit for extracellular calcium. Preventing calcium entry during the repeated PEMF exposure with the co-administration of EGTA or TRP channel antagonists precluded the inhibition of differentiation. This study highlights the intricacies of calcium homeostasis during early chondrogenesis and the constraints that are placed on PEMF-based therapeutic strategies aimed at promoting MSC chondrogenesis. The demonstrated efficacy of our optimized PEMF regimens has clear clinical implications for future regenerative strategies for cartilage. Nature Publishing Group 2017 Article PeerReviewed application/pdf en http://eprints.um.edu.my/19065/1/Enhancement_of_mesenchymal_stem_cell_chondrogenesis_with_short-term_low_intensity_pulsed_electromagnetic_fields.pdf Parate, D. and Franco-Obregón, A. and Fröhlich, J. and Beyer, C. and Abbas, A.A. and Kamarul, Tunku and Hui, J.H.P. and Yang, Z. (2017) Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields. Scientific Reports, 7 (1). p. 9421. ISSN 2045-2322 http://dx.doi.org/10.1038/s41598-017-09892-w doi:10.1038/s41598-017-09892-w
institution Universiti Malaya
building UM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaya
content_source UM Research Repository
url_provider http://eprints.um.edu.my/
language English
topic R Medicine
spellingShingle R Medicine
Parate, D.
Franco-Obregón, A.
Fröhlich, J.
Beyer, C.
Abbas, A.A.
Kamarul, Tunku
Hui, J.H.P.
Yang, Z.
Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields
description Pulse electromagnetic fields (PEMFs) have been shown to recruit calcium-signaling cascades common to chondrogenesis. Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. MSCs undergoing chondrogenesis are preferentially responsive to an electromagnetic efficacy window defined by field amplitude, duration and frequency of exposure. Contrary to conventional practice of administering prolonged and repetitive exposures to PEMFs, optimal chondrogenic outcome is achieved in response to brief (10 minutes), low intensity (2 mT) exposure to 6 ms bursts of magnetic pulses, at 15 Hz, administered only once at the onset of chondrogenic induction. By contrast, repeated exposures diminished chondrogenic outcome and could be attributed to calcium entry after the initial induction. Transient receptor potential (TRP) channels appear to mediate these aspects of PEMF stimulation, serving as a conduit for extracellular calcium. Preventing calcium entry during the repeated PEMF exposure with the co-administration of EGTA or TRP channel antagonists precluded the inhibition of differentiation. This study highlights the intricacies of calcium homeostasis during early chondrogenesis and the constraints that are placed on PEMF-based therapeutic strategies aimed at promoting MSC chondrogenesis. The demonstrated efficacy of our optimized PEMF regimens has clear clinical implications for future regenerative strategies for cartilage.
format Article
author Parate, D.
Franco-Obregón, A.
Fröhlich, J.
Beyer, C.
Abbas, A.A.
Kamarul, Tunku
Hui, J.H.P.
Yang, Z.
author_facet Parate, D.
Franco-Obregón, A.
Fröhlich, J.
Beyer, C.
Abbas, A.A.
Kamarul, Tunku
Hui, J.H.P.
Yang, Z.
author_sort Parate, D.
title Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields
title_short Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields
title_full Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields
title_fullStr Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields
title_full_unstemmed Enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields
title_sort enhancement of mesenchymal stem cell chondrogenesis with short-term low intensity pulsed electromagnetic fields
publisher Nature Publishing Group
publishDate 2017
url http://eprints.um.edu.my/19065/1/Enhancement_of_mesenchymal_stem_cell_chondrogenesis_with_short-term_low_intensity_pulsed_electromagnetic_fields.pdf
http://eprints.um.edu.my/19065/
http://dx.doi.org/10.1038/s41598-017-09892-w
_version_ 1643690876867706880
score 13.211869

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