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Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir

Eggshell membranes (ESM) are considered food waste in the egg processing industry. Recently, researchers have shown an increased interest in enzymatically treated ESM hydrolysate (ESMH). This is because eggshell biopeptides may help to improve eggshell biocalcium bioavailability, and calcium-binding...

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Main Author: Abd Kadir, Nur Farah Syahirah
Format: Thesis
Language:English
Published: 2023
Subjects:
QD Chemistry
Online Access:https://ir.uitm.edu.my/id/eprint/91033/1/91033.pdf
https://ir.uitm.edu.my/id/eprint/91033/
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spelling my.uitm.ir.910332024-07-22T02:49:15Z https://ir.uitm.edu.my/id/eprint/91033/ Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir Abd Kadir, Nur Farah Syahirah QD Chemistry Eggshell membranes (ESM) are considered food waste in the egg processing industry. Recently, researchers have shown an increased interest in enzymatically treated ESM hydrolysate (ESMH). This is because eggshell biopeptides may help to improve eggshell biocalcium bioavailability, and calcium-binding capacity, but the data were limited. Thus, in this study, the ESM, and its hydrolysates (Alcalase-treated eggshell membrane hydrolysate (Al-ESMH), and Trypsin-treated eggshell membrane hydrolysate (Tr-ESMH)) were characterised. Subsequently, the characterisation of the ESM, and ESMHs was carried out using ultra-violet visible spectroscopy (UV-Vis), and Fourier transform infrared spectroscopy (FTIR). The calcium-binding capacity was performed by its protocol. Thereafter, the calcium bioavailability was carried out using in vitro equilibrium dialysis through a semipermeable membrane. The calcium quantitation was performed using atomic absorption spectroscopy (AAS). The obtained results from UV spectra showed two intense absorption peaks at 238 nm, and 280 nm. The second peak at 280 nm was used to quantify the soluble protein in ESMHs. 2023 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/91033/1/91033.pdf Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir. (2023) Masters thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/91033.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Abd Kadir, Nur Farah Syahirah
Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir
description Eggshell membranes (ESM) are considered food waste in the egg processing industry. Recently, researchers have shown an increased interest in enzymatically treated ESM hydrolysate (ESMH). This is because eggshell biopeptides may help to improve eggshell biocalcium bioavailability, and calcium-binding capacity, but the data were limited. Thus, in this study, the ESM, and its hydrolysates (Alcalase-treated eggshell membrane hydrolysate (Al-ESMH), and Trypsin-treated eggshell membrane hydrolysate (Tr-ESMH)) were characterised. Subsequently, the characterisation of the ESM, and ESMHs was carried out using ultra-violet visible spectroscopy (UV-Vis), and Fourier transform infrared spectroscopy (FTIR). The calcium-binding capacity was performed by its protocol. Thereafter, the calcium bioavailability was carried out using in vitro equilibrium dialysis through a semipermeable membrane. The calcium quantitation was performed using atomic absorption spectroscopy (AAS). The obtained results from UV spectra showed two intense absorption peaks at 238 nm, and 280 nm. The second peak at 280 nm was used to quantify the soluble protein in ESMHs.
format Thesis
author Abd Kadir, Nur Farah Syahirah
author_facet Abd Kadir, Nur Farah Syahirah
author_sort Abd Kadir, Nur Farah Syahirah
title Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir
title_short Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir
title_full Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir
title_fullStr Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir
title_full_unstemmed Enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / Nur Farah Syahirah Abd Kadir
title_sort enzymatically hydrolysed eggshell membranes biopeptides-biocalcium complex characterisation, binding capacity and bioavailability / nur farah syahirah abd kadir
publishDate 2023
url https://ir.uitm.edu.my/id/eprint/91033/1/91033.pdf
https://ir.uitm.edu.my/id/eprint/91033/
_version_ 1806422074397294592
score 13.211869

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