Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim

Watermelon or Citrullus lanatus (Thunb.) Matsum. And Nakai is a good source of phytonutrients and antioxidants such as Vitamin C, water soluble vitamins which is an essential micronutrient that have several important biological functions. Vitamin C as a potent antioxidant can eliminates reactive oxy...

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Main Author: Abd Rahim, Nurarifah
Format: Thesis
Language:English
Published: 2017
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Online Access:https://ir.uitm.edu.my/id/eprint/50238/1/50238.pdf
https://ir.uitm.edu.my/id/eprint/50238/
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spelling my.uitm.ir.502382021-09-09T01:45:48Z https://ir.uitm.edu.my/id/eprint/50238/ Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim Abd Rahim, Nurarifah Botanical chemistry. Phytochemicals Materia medica Fruit and fruit culture Watermelon or Citrullus lanatus (Thunb.) Matsum. And Nakai is a good source of phytonutrients and antioxidants such as Vitamin C, water soluble vitamins which is an essential micronutrient that have several important biological functions. Vitamin C as a potent antioxidant can eliminates reactive oxygen and nitrogen species and protective against oxidative damage. Due to lack of an enzyme human cannot synthesize ascorbic acid hence, it has to be supplemented through fruits such as watermelon. However, there are insufficient studies on ascorbic acid identification and quantification by using HPLC with better extraction method that can prevent ascorbic acid oxidation in local yellow watermelon. Therefore, this study was conducted to identify and quantify ascorbic acid compound in local yellow watermelon by using 3 Ortho-phosphoric and 8 Acetic acid extraction with 0.1 Ortho-phosphoric : Acetonitrile (95:5) mobile phase in reverse-phase High Performance Liquid Chromatography (HPLC). The retention time for the ascorbic acid in the chromatogram is 2.798 min for both standard and sample. Standard curves were linear over the concentration range from 100 to 500 µg/mL and the limits of detection and quantification were 0.47 and 1.43 µg/mL, respectively. The method was validated by doing spike recovery. The concentration of ascorbic acid in yellow flesh of local watermelon was found to be 19.05 µg/mL with percentage of 0.00002%. In conclusion, it can be stated that reverse-phase HPLC is the most preferred method to identify the presence of ascorbic acid. In addition, 3 Orthophosphoric and 8 Acetic acid in extraction method was a good method in order to prevent ascorbic acid oxidation. 2017 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/50238/1/50238.pdf ID50238 Abd Rahim, Nurarifah (2017) Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim. Degree thesis, thesis, Universiti Teknologi Mara (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Botanical chemistry. Phytochemicals
Materia medica
Fruit and fruit culture
spellingShingle Botanical chemistry. Phytochemicals
Materia medica
Fruit and fruit culture
Abd Rahim, Nurarifah
Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
description Watermelon or Citrullus lanatus (Thunb.) Matsum. And Nakai is a good source of phytonutrients and antioxidants such as Vitamin C, water soluble vitamins which is an essential micronutrient that have several important biological functions. Vitamin C as a potent antioxidant can eliminates reactive oxygen and nitrogen species and protective against oxidative damage. Due to lack of an enzyme human cannot synthesize ascorbic acid hence, it has to be supplemented through fruits such as watermelon. However, there are insufficient studies on ascorbic acid identification and quantification by using HPLC with better extraction method that can prevent ascorbic acid oxidation in local yellow watermelon. Therefore, this study was conducted to identify and quantify ascorbic acid compound in local yellow watermelon by using 3 Ortho-phosphoric and 8 Acetic acid extraction with 0.1 Ortho-phosphoric : Acetonitrile (95:5) mobile phase in reverse-phase High Performance Liquid Chromatography (HPLC). The retention time for the ascorbic acid in the chromatogram is 2.798 min for both standard and sample. Standard curves were linear over the concentration range from 100 to 500 µg/mL and the limits of detection and quantification were 0.47 and 1.43 µg/mL, respectively. The method was validated by doing spike recovery. The concentration of ascorbic acid in yellow flesh of local watermelon was found to be 19.05 µg/mL with percentage of 0.00002%. In conclusion, it can be stated that reverse-phase HPLC is the most preferred method to identify the presence of ascorbic acid. In addition, 3 Orthophosphoric and 8 Acetic acid in extraction method was a good method in order to prevent ascorbic acid oxidation.
format Thesis
author Abd Rahim, Nurarifah
author_facet Abd Rahim, Nurarifah
author_sort Abd Rahim, Nurarifah
title Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_short Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_full Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_fullStr Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_full_unstemmed Detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (HPLC) / Nurarifah Abd Rahim
title_sort detection and quantification of ascorbic acid in yellow crimson watermelon (citrullus lanatus) by using reverse-phase high performance liquid chromatography (hplc) / nurarifah abd rahim
publishDate 2017
url https://ir.uitm.edu.my/id/eprint/50238/1/50238.pdf
https://ir.uitm.edu.my/id/eprint/50238/
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