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Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid

Lactobacillus sp. is lactic acid bacteria that act as probiotics which provide range of health benefits especially to human digestion. Lactobacillus sp. can be found on various dairy products sources such as fresh milk. The aim of this study was to isolate and identify Lactobacillus sp. from cow an...

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Main Author: A.Rasid, Syazrah Najiha
Format: Student Project
Language:English
Published: 2018
Subjects:
Laboratories. General works
Extraction (Chemistry)
Bacteria
Online Access:http://ir.uitm.edu.my/id/eprint/34249/1/34249.pdf
http://ir.uitm.edu.my/id/eprint/34249/
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spelling my.uitm.ir.342492020-09-03T09:16:36Z http://ir.uitm.edu.my/id/eprint/34249/ Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid A.Rasid, Syazrah Najiha Laboratories. General works Extraction (Chemistry) Bacteria Lactobacillus sp. is lactic acid bacteria that act as probiotics which provide range of health benefits especially to human digestion. Lactobacillus sp. can be found on various dairy products sources such as fresh milk. The aim of this study was to isolate and identify Lactobacillus sp. from cow and goat fresh milk. Biochemical test was used for the morphological identification of Lactobacillus sp. from animal fresh milk. The results obtained in the study confirmed that the tested cow and goat fresh milk consist of Lactobacillus sp. Identification of Lactobacillus sp. was carried out using molecular method, Polymerase Chain Reaction (PCR) with specific primers Lacto-16S-F (5’ GGA ATC TTC CAC AAT GGA CG 3’) and Lacto-16S-R (5’ CGC TTC ACG CCC AAT AAA TCC GG 3’). All samples showed positive results of DNA band on gel electrophoresis and amplicon size was 216 bp. Lactobacillus sp. was successfully identified in animal fresh milk. Finding in this study suggested to carry out DNA sequencing from the PCR products for the identification of Lactobacillus sp. on the species level. 2018 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/34249/1/34249.pdf A.Rasid, Syazrah Najiha (2018) Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Laboratories. General works
Extraction (Chemistry)
Bacteria
spellingShingle Laboratories. General works
Extraction (Chemistry)
Bacteria
A.Rasid, Syazrah Najiha
Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid
description Lactobacillus sp. is lactic acid bacteria that act as probiotics which provide range of health benefits especially to human digestion. Lactobacillus sp. can be found on various dairy products sources such as fresh milk. The aim of this study was to isolate and identify Lactobacillus sp. from cow and goat fresh milk. Biochemical test was used for the morphological identification of Lactobacillus sp. from animal fresh milk. The results obtained in the study confirmed that the tested cow and goat fresh milk consist of Lactobacillus sp. Identification of Lactobacillus sp. was carried out using molecular method, Polymerase Chain Reaction (PCR) with specific primers Lacto-16S-F (5’ GGA ATC TTC CAC AAT GGA CG 3’) and Lacto-16S-R (5’ CGC TTC ACG CCC AAT AAA TCC GG 3’). All samples showed positive results of DNA band on gel electrophoresis and amplicon size was 216 bp. Lactobacillus sp. was successfully identified in animal fresh milk. Finding in this study suggested to carry out DNA sequencing from the PCR products for the identification of Lactobacillus sp. on the species level.
format Student Project
author A.Rasid, Syazrah Najiha
author_facet A.Rasid, Syazrah Najiha
author_sort A.Rasid, Syazrah Najiha
title Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid
title_short Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid
title_full Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid
title_fullStr Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid
title_full_unstemmed Molecular identification of lactobacillus Sp. from animal fresh milk / Syazrah Najiha A.Rasid
title_sort molecular identification of lactobacillus sp. from animal fresh milk / syazrah najiha a.rasid
publishDate 2018
url http://ir.uitm.edu.my/id/eprint/34249/1/34249.pdf
http://ir.uitm.edu.my/id/eprint/34249/
_version_ 1685651168472072192
score 13.211869

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