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Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah

Lactobacilli are gram positive, facultative bacteria which convert lactose and simple sugars to lactic acid. Species belonging to the genus Lactobacillus are used specifically in dairy products. The main purpose of this study was to isolate the Lactobacillus sp. from the dairy product samples and to...

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Main Author: Iskandarshah, Nurul Fatin
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2015
Subjects:
Extraction (Chemistry)
Bacteria
Online Access:http://ir.uitm.edu.my/id/eprint/25132/1/PPb_NURUL%20FATIN%20ISKANDARSHAH%20AS%20N%2015_5.pdf
http://ir.uitm.edu.my/id/eprint/25132/
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spelling my.uitm.ir.251322019-08-09T02:41:16Z http://ir.uitm.edu.my/id/eprint/25132/ Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah Iskandarshah, Nurul Fatin Extraction (Chemistry) Bacteria Lactobacilli are gram positive, facultative bacteria which convert lactose and simple sugars to lactic acid. Species belonging to the genus Lactobacillus are used specifically in dairy products. The main purpose of this study was to isolate the Lactobacillus sp. from the dairy product samples and to identify Lactobacillus sp. in dairy product samples by using PCR method. A total of four samples from dairy products which are yogurt, vitagen, yakult, goat milk and three Lactobacillus sp. strains were used in this study. Dairy product samples were purchased at Econsave supermarket and Lactobacillus strains were obtained from Makmal Halal UNISEL, Selangor. Streak plate method was carried out on MRS agar plates to isolate Lactobacillus sp. from the samples. The genomic DNA from these samples were extracted using RTP® Bacteria DNA Mini Kit and were subjected to PCR amplification for the detection of Lactobacillus species using specific primers. All samples showed positive result of DNA band on agarose gel electrophoresis. The extraction DNA method using RTP® Bacteria DNA Mini Kit is suggested for high quality and more reliable result. Faculty of Applied Sciences 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/25132/1/PPb_NURUL%20FATIN%20ISKANDARSHAH%20AS%20N%2015_5.pdf Iskandarshah, Nurul Fatin (2015) Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah. [Student Project] (Submitted)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Extraction (Chemistry)
Bacteria
spellingShingle Extraction (Chemistry)
Bacteria
Iskandarshah, Nurul Fatin
Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
description Lactobacilli are gram positive, facultative bacteria which convert lactose and simple sugars to lactic acid. Species belonging to the genus Lactobacillus are used specifically in dairy products. The main purpose of this study was to isolate the Lactobacillus sp. from the dairy product samples and to identify Lactobacillus sp. in dairy product samples by using PCR method. A total of four samples from dairy products which are yogurt, vitagen, yakult, goat milk and three Lactobacillus sp. strains were used in this study. Dairy product samples were purchased at Econsave supermarket and Lactobacillus strains were obtained from Makmal Halal UNISEL, Selangor. Streak plate method was carried out on MRS agar plates to isolate Lactobacillus sp. from the samples. The genomic DNA from these samples were extracted using RTP® Bacteria DNA Mini Kit and were subjected to PCR amplification for the detection of Lactobacillus species using specific primers. All samples showed positive result of DNA band on agarose gel electrophoresis. The extraction DNA method using RTP® Bacteria DNA Mini Kit is suggested for high quality and more reliable result.
format Student Project
author Iskandarshah, Nurul Fatin
author_facet Iskandarshah, Nurul Fatin
author_sort Iskandarshah, Nurul Fatin
title Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_short Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_full Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_fullStr Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_full_unstemmed Isolation and identification of lactobacillus sp. from dairy products using pcr method / Nurul Fatin Iskandarshah
title_sort isolation and identification of lactobacillus sp. from dairy products using pcr method / nurul fatin iskandarshah
publisher Faculty of Applied Sciences
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/25132/1/PPb_NURUL%20FATIN%20ISKANDARSHAH%20AS%20N%2015_5.pdf
http://ir.uitm.edu.my/id/eprint/25132/
_version_ 1685649956872912896
score 13.211869

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