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Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin

People nowadays have very little consideration on food safety and practice poor eating habits such as eating raw or undercooked seafood. There is a presence of microorganism that is said to be opportunistic pathogens such as in cockles, there is a presence of Vibrio spp. which can cause vibriosis. T...

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Main Author: Kamaruddin, Nur Hajaratul Aswat
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2014
Subjects:
Microorganisms
QR Microbiology
Online Access:http://ir.uitm.edu.my/id/eprint/24742/1/PPb_NUR%20HAJARATUL%20ASWAT%20KAMARUDDIN%20AS%20N%2014_5.pdf
http://ir.uitm.edu.my/id/eprint/24742/
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spelling my.uitm.ir.247422019-07-19T00:45:14Z http://ir.uitm.edu.my/id/eprint/24742/ Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin Kamaruddin, Nur Hajaratul Aswat Microorganisms QR Microbiology People nowadays have very little consideration on food safety and practice poor eating habits such as eating raw or undercooked seafood. There is a presence of microorganism that is said to be opportunistic pathogens such as in cockles, there is a presence of Vibrio spp. which can cause vibriosis. This study is to detect the presence of V spp. and V cholerae in cockles at three different retails in Kuala Pilah, Negeri Sembilan and the antibiotic susceptibility of that species. The findings of the study show that there is more than 2.4 x I 0 10 MPN/g in IO g of cockles from all three places. The result obtained by using Most Probable Number (MPN) method. Then in specifically determining of V cholerae, night market cockles were found to have highest rate of contamination with 71.43%. In order to clarify the presence of V spp. and V cholerae in cockles, Polymerase Chain Reaction (PCR) and visualization by Gel Electrophoresis had been done, proving that the presence of both species were positive. Lastly, for antibiotic test, the Multiple Antibiotic Resistance (MAR) and Disk Diffusion method were used and seven out of 14 types of antibiotics were found to be resistance since the MAR index was between 0.2 to 0.5. Bacitracin, Vancomycin, Furazididone, Tetracycline and Erythromycin are highly resistance to Vibrio spp. where Bacitracin is the best antibiotic to treat that species. Faculty of Applied Sciences 2014 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24742/1/PPb_NUR%20HAJARATUL%20ASWAT%20KAMARUDDIN%20AS%20N%2014_5.pdf Kamaruddin, Nur Hajaratul Aswat (2014) Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin. [Student Project] (Submitted)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Microorganisms
QR Microbiology
spellingShingle Microorganisms
QR Microbiology
Kamaruddin, Nur Hajaratul Aswat
Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
description People nowadays have very little consideration on food safety and practice poor eating habits such as eating raw or undercooked seafood. There is a presence of microorganism that is said to be opportunistic pathogens such as in cockles, there is a presence of Vibrio spp. which can cause vibriosis. This study is to detect the presence of V spp. and V cholerae in cockles at three different retails in Kuala Pilah, Negeri Sembilan and the antibiotic susceptibility of that species. The findings of the study show that there is more than 2.4 x I 0 10 MPN/g in IO g of cockles from all three places. The result obtained by using Most Probable Number (MPN) method. Then in specifically determining of V cholerae, night market cockles were found to have highest rate of contamination with 71.43%. In order to clarify the presence of V spp. and V cholerae in cockles, Polymerase Chain Reaction (PCR) and visualization by Gel Electrophoresis had been done, proving that the presence of both species were positive. Lastly, for antibiotic test, the Multiple Antibiotic Resistance (MAR) and Disk Diffusion method were used and seven out of 14 types of antibiotics were found to be resistance since the MAR index was between 0.2 to 0.5. Bacitracin, Vancomycin, Furazididone, Tetracycline and Erythromycin are highly resistance to Vibrio spp. where Bacitracin is the best antibiotic to treat that species.
format Student Project
author Kamaruddin, Nur Hajaratul Aswat
author_facet Kamaruddin, Nur Hajaratul Aswat
author_sort Kamaruddin, Nur Hajaratul Aswat
title Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_short Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_full Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_fullStr Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_full_unstemmed Prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in Kuala Pilah, Negeri Sembilan. / Nur Hajaratul Aswat Kamaruddin
title_sort prevalence and antibiotic susceptibility of vibrio spp. and vibrio cholerae in cockles (anadara granosa) at three retail levels in kuala pilah, negeri sembilan. / nur hajaratul aswat kamaruddin
publisher Faculty of Applied Sciences
publishDate 2014
url http://ir.uitm.edu.my/id/eprint/24742/1/PPb_NUR%20HAJARATUL%20ASWAT%20KAMARUDDIN%20AS%20N%2014_5.pdf
http://ir.uitm.edu.my/id/eprint/24742/
_version_ 1685649901771292672
score 13.211869

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