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Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe

Protease was extracted from bilimbi {Averrhoa bilimbi L.) and the juice obtained was purified by using two different concentrations of ammonium sulfate solution (40 and 60% (w/v)). The proteases were analysed for pH stability, temperature stability, storage stability, SDS-PAGE (molecular weight dist...

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Main Author: Mohamad Kharoe, Nur ‘Ain
Format: Student Project
Language:English
Published: 2013
Subjects:
Extraction (Chemistry)
Extraction
Online Access:https://ir.uitm.edu.my/id/eprint/109111/1/109111.pdf
https://ir.uitm.edu.my/id/eprint/109111/
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spelling my.uitm.ir.1091112025-01-15T06:02:48Z https://ir.uitm.edu.my/id/eprint/109111/ Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe Mohamad Kharoe, Nur ‘Ain Extraction (Chemistry) Extraction Protease was extracted from bilimbi {Averrhoa bilimbi L.) and the juice obtained was purified by using two different concentrations of ammonium sulfate solution (40 and 60% (w/v)). The proteases were analysed for pH stability, temperature stability, storage stability, SDS-PAGE (molecular weight distribution), protein concentration and protein content. Protein content of bilimbi is 0.89 g. Protease activity at both ripening stage and 40 and 60% ammonium sulfate purification was optimum at pH 2 to 4 and at temperature 30 to 50°C. As for storage stability at 4 °C, the protease activity decreased with storage time. Molecular weight distribution indicated that the proteases protein bands fall between 10 to 220 kDa with protein band at 50 kDa seen in both unripe and ripe bilimbi proteases purified with 40%) ammonium sulfate. As the concentration of ammonium sulfate decreases, the protein concentration increase at both ripening stages. Purification using 40%> ammonium sulfate precipitation could be a successful method to purify proteases from bilimbi especially from the unripe stage. 2013 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109111/1/109111.pdf Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe. (2013) [Student Project] <http://terminalib.uitm.edu.my/109111.pdf> (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Extraction (Chemistry)
Extraction
spellingShingle Extraction (Chemistry)
Extraction
Mohamad Kharoe, Nur ‘Ain
Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe
description Protease was extracted from bilimbi {Averrhoa bilimbi L.) and the juice obtained was purified by using two different concentrations of ammonium sulfate solution (40 and 60% (w/v)). The proteases were analysed for pH stability, temperature stability, storage stability, SDS-PAGE (molecular weight distribution), protein concentration and protein content. Protein content of bilimbi is 0.89 g. Protease activity at both ripening stage and 40 and 60% ammonium sulfate purification was optimum at pH 2 to 4 and at temperature 30 to 50°C. As for storage stability at 4 °C, the protease activity decreased with storage time. Molecular weight distribution indicated that the proteases protein bands fall between 10 to 220 kDa with protein band at 50 kDa seen in both unripe and ripe bilimbi proteases purified with 40%) ammonium sulfate. As the concentration of ammonium sulfate decreases, the protein concentration increase at both ripening stages. Purification using 40%> ammonium sulfate precipitation could be a successful method to purify proteases from bilimbi especially from the unripe stage.
format Student Project
author Mohamad Kharoe, Nur ‘Ain
author_facet Mohamad Kharoe, Nur ‘Ain
author_sort Mohamad Kharoe, Nur ‘Ain
title Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe
title_short Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe
title_full Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe
title_fullStr Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe
title_full_unstemmed Extraction of protease from bilimbi (Averrhoa bilimbi L.) / Nur ‘Ain Mohamad Kharoe
title_sort extraction of protease from bilimbi (averrhoa bilimbi l.) / nur ‘ain mohamad kharoe
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109111/1/109111.pdf
https://ir.uitm.edu.my/id/eprint/109111/
_version_ 1823097872609968128
score 13.235362

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