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Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi

The study of the antimicrobial potency of the two aromatic vegetables Allium sativum Linn and Zingiber officinale Roscoe has been investigated against the two most common food spoilage bacteria, Escherichia coli and Salmonella enterica. The antibacterial activity was determined by the disc diffusion...

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Main Author: Apandi, Siti Noor Aliza
Format: Student Project
Language:English
Published: 2016
Subjects:
Bacteria
Online Access:https://ir.uitm.edu.my/id/eprint/108362/1/108362.pdf
https://ir.uitm.edu.my/id/eprint/108362/
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spelling my.uitm.ir.1083622025-02-27T23:13:20Z https://ir.uitm.edu.my/id/eprint/108362/ Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi Apandi, Siti Noor Aliza Bacteria The study of the antimicrobial potency of the two aromatic vegetables Allium sativum Linn and Zingiber officinale Roscoe has been investigated against the two most common food spoilage bacteria, Escherichia coli and Salmonella enterica. The antibacterial activity was determined by the disc diffusion method. Kirby-Bauer method was used to determine the susceptibility of the bacteria toward the aqueous extract of A.sativum and Z.officinale. The average inhibition zone of 300 mg/ml fresh A.sativum aqueous extracts of against the E.coli is 14.2 mm (σ=0.76) and 15.3 mm (σ=0.76) for S.enterica.At 100 mg/ml dried A.sativum aqueous extract its recorded the average of the inhibition zone of against E.coliis 6.7 mm (σ=0.29)and 5.5 mm (σ=1.32)for S.enterica. The average inhibition zone of 300 mg/ml of fresh Z.officinale against E.coli is 6.8 mm (σ=0.76) and 6.0 mm (σ=1.50) for S.enterica. The average zone of inhibition of 100 mg/ml of dried Z.officinale against E.coli is 12.2 mm (σ=0.29) and 11.5 mm (σ=0.50) for S.enterica. The minimum inhibitory concentration (MIC) of fresh A.sativum, dried A.sativum, fresh Z.officinale, and dried Z.officinale was recorded at 9.37 mg/ml, 50.00 mg/ml, 12.50 mg/ml, and 75.00 mg/ml respectively. These concentrations were used to disinfect the chicken meat for 90 minutes of exposure. Theenumeration of bacteria using pour plate method were performed every 30 minutes. The total colony count of untreated chicken meat were recorded at μ=67.25 per gram. The average total colony count after 60 minutes of fresh A.sativum, dried A.sativum, fresh Z.officinale, and dried Z.officinale extracts exposures were 1.6, 30.3, 17.6 and 9.0 respectively. At 90th minutes, the average total plate count of fresh A.sativum, dried A.sativum, fresh Z.officinale, and dried Z.officinale extracts exposures were 18.6 CFU/ml, 23.3 CFU/ml, 36.3 CFU/ml and 55.6 CFU/ml respectively. Overall study stated fresh A.sativum aqueous extract has the potential as natural antibacteriocidal agent. From this comparative study of these natural preservative, it was conclude that the fresh A.sativum is more effective than the dried A.sativum, and dried Z.officinale were stronger antibacterial than the fresh Z.officinale extracts. This comparative showed the fresh A.sativum extract is most effective, which inhibit the bacterial growth at lowest concentration of 9.37 mg/ml. It is recommended to increase the concentration of the bioactive compound, for both aromatic vegetables to obtain the higher antibacterial activity, for a better control of the bacterial growth. 2016 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/108362/1/108362.pdf Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi. (2016) [Student Project] (Submitted)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Bacteria
spellingShingle Bacteria
Apandi, Siti Noor Aliza
Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi
description The study of the antimicrobial potency of the two aromatic vegetables Allium sativum Linn and Zingiber officinale Roscoe has been investigated against the two most common food spoilage bacteria, Escherichia coli and Salmonella enterica. The antibacterial activity was determined by the disc diffusion method. Kirby-Bauer method was used to determine the susceptibility of the bacteria toward the aqueous extract of A.sativum and Z.officinale. The average inhibition zone of 300 mg/ml fresh A.sativum aqueous extracts of against the E.coli is 14.2 mm (σ=0.76) and 15.3 mm (σ=0.76) for S.enterica.At 100 mg/ml dried A.sativum aqueous extract its recorded the average of the inhibition zone of against E.coliis 6.7 mm (σ=0.29)and 5.5 mm (σ=1.32)for S.enterica. The average inhibition zone of 300 mg/ml of fresh Z.officinale against E.coli is 6.8 mm (σ=0.76) and 6.0 mm (σ=1.50) for S.enterica. The average zone of inhibition of 100 mg/ml of dried Z.officinale against E.coli is 12.2 mm (σ=0.29) and 11.5 mm (σ=0.50) for S.enterica. The minimum inhibitory concentration (MIC) of fresh A.sativum, dried A.sativum, fresh Z.officinale, and dried Z.officinale was recorded at 9.37 mg/ml, 50.00 mg/ml, 12.50 mg/ml, and 75.00 mg/ml respectively. These concentrations were used to disinfect the chicken meat for 90 minutes of exposure. Theenumeration of bacteria using pour plate method were performed every 30 minutes. The total colony count of untreated chicken meat were recorded at μ=67.25 per gram. The average total colony count after 60 minutes of fresh A.sativum, dried A.sativum, fresh Z.officinale, and dried Z.officinale extracts exposures were 1.6, 30.3, 17.6 and 9.0 respectively. At 90th minutes, the average total plate count of fresh A.sativum, dried A.sativum, fresh Z.officinale, and dried Z.officinale extracts exposures were 18.6 CFU/ml, 23.3 CFU/ml, 36.3 CFU/ml and 55.6 CFU/ml respectively. Overall study stated fresh A.sativum aqueous extract has the potential as natural antibacteriocidal agent. From this comparative study of these natural preservative, it was conclude that the fresh A.sativum is more effective than the dried A.sativum, and dried Z.officinale were stronger antibacterial than the fresh Z.officinale extracts. This comparative showed the fresh A.sativum extract is most effective, which inhibit the bacterial growth at lowest concentration of 9.37 mg/ml. It is recommended to increase the concentration of the bioactive compound, for both aromatic vegetables to obtain the higher antibacterial activity, for a better control of the bacterial growth.
format Student Project
author Apandi, Siti Noor Aliza
author_facet Apandi, Siti Noor Aliza
author_sort Apandi, Siti Noor Aliza
title Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi
title_short Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi
title_full Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi
title_fullStr Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi
title_full_unstemmed Antibacterial activity of fresh and dried samples of Allium sativum Linn and Zingiber officinale Roscoe against Salmonella enterica and Escherichia coli on raw chicken meat / Siti Noor Aliza Apandi
title_sort antibacterial activity of fresh and dried samples of allium sativum linn and zingiber officinale roscoe against salmonella enterica and escherichia coli on raw chicken meat / siti noor aliza apandi
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/108362/1/108362.pdf
https://ir.uitm.edu.my/id/eprint/108362/
_version_ 1825814803886637056
score 13.244413

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