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Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine

Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techn...

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Main Authors: Ramanathan, Babu *, Poh, Chit Laa *, Kirk, Kristin, McBride, William John Hannan, Aaskov, John, Grollo, Lara
Format: Article
Language:English
Published: PLOS 2016
Subjects:
QR Microbiology
R Medicine (General)
Online Access:http://eprints.sunway.edu.my/454/1/Ramanathan%202016%20Synthetic%20B.pdf
http://eprints.sunway.edu.my/454/
http://dx.doi.org/10.1371/journal.pone.0155900
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spelling my.sunway.eprints.4542019-07-04T08:04:59Z http://eprints.sunway.edu.my/454/ Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine Ramanathan, Babu * Poh, Chit Laa * Kirk, Kristin McBride, William John Hannan Aaskov, John Grollo, Lara QR Microbiology R Medicine (General) Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine. PLOS 2016 Article PeerReviewed text en http://eprints.sunway.edu.my/454/1/Ramanathan%202016%20Synthetic%20B.pdf Ramanathan, Babu * and Poh, Chit Laa * and Kirk, Kristin and McBride, William John Hannan and Aaskov, John and Grollo, Lara (2016) Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine. PLOS ONE, 11 (5). e0155900. ISSN 1932-6203 http://dx.doi.org/10.1371/journal.pone.0155900 doi:10.1371/journal.pone.0155900
institution Sunway University
building Sunway Campus Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Sunway University
content_source Sunway Institutional Repository
url_provider http://eprints.sunway.edu.my/
language English
topic QR Microbiology
R Medicine (General)
spellingShingle QR Microbiology
R Medicine (General)
Ramanathan, Babu *
Poh, Chit Laa *
Kirk, Kristin
McBride, William John Hannan
Aaskov, John
Grollo, Lara
Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
description Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine.
format Article
author Ramanathan, Babu *
Poh, Chit Laa *
Kirk, Kristin
McBride, William John Hannan
Aaskov, John
Grollo, Lara
author_facet Ramanathan, Babu *
Poh, Chit Laa *
Kirk, Kristin
McBride, William John Hannan
Aaskov, John
Grollo, Lara
author_sort Ramanathan, Babu *
title Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
title_short Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
title_full Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
title_fullStr Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
title_full_unstemmed Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
title_sort synthetic b-cell epitopes eliciting cross-neutralizing antibodies: strategies for future dengue vaccine
publisher PLOS
publishDate 2016
url http://eprints.sunway.edu.my/454/1/Ramanathan%202016%20Synthetic%20B.pdf
http://eprints.sunway.edu.my/454/
http://dx.doi.org/10.1371/journal.pone.0155900
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score 13.211869

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