Synthetic B-Cell epitopes eliciting cross-neutralizing antibodies: Strategies for future dengue vaccine
Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techn...
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| 主要な著者: | , , , , , |
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| フォーマット: | 論文 |
| 言語: | English |
| 出版事項: |
PLOS
2016
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| 主題: | |
| オンライン･アクセス: | http://eprints.sunway.edu.my/454/1/Ramanathan%202016%20Synthetic%20B.pdf http://eprints.sunway.edu.my/454/ http://dx.doi.org/10.1371/journal.pone.0155900 |
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| 要約: | Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides
representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine
constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope
mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine. |
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