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Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth

Epstein-Barr virus (EBV) is one of the common human herpesvirus types in the world. EBV is known to infect more than 95% of adults in the world. Te virus mainly infects B lymphocytes and could immortalize and transform the cells into EBVbearing lymphoblastoid cell lines (LCLs). Limited studies have...

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Main Authors: Yap, Hooi Yeen *, Siow, Thin Sam, Chow, Sook Khuan *, Teow, Sin Yeang *
Format: Article
Language:English
Published: Hindawi 2019
Subjects:
QR355 Virology
Online Access:http://eprints.sunway.edu.my/1153/1/Teow%20Sin%20Yeang%20Epstein%20Barr%20Virus.pdf
http://eprints.sunway.edu.my/1153/
http://doi.org/10.1155/2019/6464521
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spelling my.sunway.eprints.11532019-11-28T04:08:49Z http://eprints.sunway.edu.my/1153/ Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth Yap, Hooi Yeen * Siow, Thin Sam Chow, Sook Khuan * Teow, Sin Yeang * QR355 Virology Epstein-Barr virus (EBV) is one of the common human herpesvirus types in the world. EBV is known to infect more than 95% of adults in the world. Te virus mainly infects B lymphocytes and could immortalize and transform the cells into EBVbearing lymphoblastoid cell lines (LCLs). Limited studies have been focused on characterizing the surface marker expression of the immortalized LCLs. Tis study demonstrates the generation of 15 LCLs from sixteen rheumatoid arthritis (RA) patients and a healthy volunteer using B95-8 marmoset-derived EBV. Te success rate of LCL generation was 88.23%. All CD19+ LCLs expressed CD23 (16.94-58.9%) and CD27 (15.74-80.89%) on cell surface. Our data demonstrated two distinct categories of LCLs (fast- and slow-growing) (p<0.05) based on their doubling time. Te slow-growing LCLs showed lower CD23 level (35.28%) compared to fast-growing LCLs (42.39%). In contrast, the slow-growing LCLs showed higher percentage in both CD27 alone and CD23+CD27+ in combination. Overall, these fndings may suggest the correlations of cellular CD23 and CD27 expression with the proliferation rate of the generated LCLs. Increase expression of CD23 may play a role in EBV immortalization of B-cells and the growth and maintenance of the EBV-transformed LCLs while CD27 expression might have inhibitory efects on LCL proliferation. Further investigations are warranted to these speculations. Hindawi 2019-03-28 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1153/1/Teow%20Sin%20Yeang%20Epstein%20Barr%20Virus.pdf Yap, Hooi Yeen * and Siow, Thin Sam and Chow, Sook Khuan * and Teow, Sin Yeang * (2019) Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth. Advances in Virology, 2019. pp. 1-9. ISSN 1687-8639 http://doi.org/10.1155/2019/6464521 doi:10.1155/2019/6464521
institution Sunway University
building Sunway Campus Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Sunway University
content_source Sunway Institutional Repository
url_provider http://eprints.sunway.edu.my/
language English
topic QR355 Virology
spellingShingle QR355 Virology
Yap, Hooi Yeen *
Siow, Thin Sam
Chow, Sook Khuan *
Teow, Sin Yeang *
Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth
description Epstein-Barr virus (EBV) is one of the common human herpesvirus types in the world. EBV is known to infect more than 95% of adults in the world. Te virus mainly infects B lymphocytes and could immortalize and transform the cells into EBVbearing lymphoblastoid cell lines (LCLs). Limited studies have been focused on characterizing the surface marker expression of the immortalized LCLs. Tis study demonstrates the generation of 15 LCLs from sixteen rheumatoid arthritis (RA) patients and a healthy volunteer using B95-8 marmoset-derived EBV. Te success rate of LCL generation was 88.23%. All CD19+ LCLs expressed CD23 (16.94-58.9%) and CD27 (15.74-80.89%) on cell surface. Our data demonstrated two distinct categories of LCLs (fast- and slow-growing) (p<0.05) based on their doubling time. Te slow-growing LCLs showed lower CD23 level (35.28%) compared to fast-growing LCLs (42.39%). In contrast, the slow-growing LCLs showed higher percentage in both CD27 alone and CD23+CD27+ in combination. Overall, these fndings may suggest the correlations of cellular CD23 and CD27 expression with the proliferation rate of the generated LCLs. Increase expression of CD23 may play a role in EBV immortalization of B-cells and the growth and maintenance of the EBV-transformed LCLs while CD27 expression might have inhibitory efects on LCL proliferation. Further investigations are warranted to these speculations.
format Article
author Yap, Hooi Yeen *
Siow, Thin Sam
Chow, Sook Khuan *
Teow, Sin Yeang *
author_facet Yap, Hooi Yeen *
Siow, Thin Sam
Chow, Sook Khuan *
Teow, Sin Yeang *
author_sort Yap, Hooi Yeen *
title Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth
title_short Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth
title_full Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth
title_fullStr Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth
title_full_unstemmed Epstein-Barr Virus- (EBV-) immortalized Lymphoblastoid Cell Lines (LCLs) express high level of CD23 but low CD27 to support their growth
title_sort epstein-barr virus- (ebv-) immortalized lymphoblastoid cell lines (lcls) express high level of cd23 but low cd27 to support their growth
publisher Hindawi
publishDate 2019
url http://eprints.sunway.edu.my/1153/1/Teow%20Sin%20Yeang%20Epstein%20Barr%20Virus.pdf
http://eprints.sunway.edu.my/1153/
http://doi.org/10.1155/2019/6464521
_version_ 1651870929126424576
score 13.211869

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