Staff View: Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model

Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model

Objectives. Te objective of the study is to evaluate the chondroprotective activity of Channa striatus (Channa) and glucosamine sulphate (glucosamine) on histomorphometric examinations, serum biomarker, and infammatory mediators in experimental osteoarthritis (OA) rabbit model. Design. Anterior cruc...

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Main Authors:	Abdul Kadir, Azidah, Abdul Kadir, Arifah, Abd Hamid, Roslida, Mat Jais, Abdul Manan, Omar, Julia, Sadagatullah, Abdul Nawfar, Badrin, Salziyan, Win, Thin Thin, Kuttulebbai Naina Mohamed Salam, Sirajudeen, Salleh, Annas
Format:	Article
Language:	English
Published:	Hindawi Publishers 2019
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/87628/1/Dr.Azidah%20paper%202019.pdf http://irep.iium.edu.my/87628/ http://doi.org/10.1155/2019/6979585
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spelling	my.iium.irep.876282021-01-11T00:23:15Z http://irep.iium.edu.my/87628/ Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model Abdul Kadir, Azidah Abdul Kadir, Arifah Abd Hamid, Roslida Mat Jais, Abdul Manan Omar, Julia Sadagatullah, Abdul Nawfar Badrin, Salziyan Win, Thin Thin Kuttulebbai Naina Mohamed Salam, Sirajudeen Salleh, Annas R Medicine (General) Objectives. Te objective of the study is to evaluate the chondroprotective activity of Channa striatus (Channa) and glucosamine sulphate (glucosamine) on histomorphometric examinations, serum biomarker, and infammatory mediators in experimental osteoarthritis (OA) rabbit model. Design. Anterior cruciate ligament transection (ACLT) was performed to induce OA in thirtythree male New Zealand white rabbits and were randomly divided into three groups: Channa, glucosamine, and control group. Te control group received drinking water and the Channa and glucosamine groups were orally administered with 51.4 mg/kg of Channa extract and 77.5 mg/kg of glucosamine sulphate in drinking water, respectively, for eight weeks and then sacrifced. Te articular cartilage was evaluated macroscopically and histologically using semiquantitative and quantitative methods. Serum cartilage oligomeric matric protein (COMP), cyclooxygenase 2 (COX-2) enzyme, and prostaglandin E2 (PGE2) were also determined. Results. Macroscopic analysis revealed that Channa group have a signifcantly lower severity grade of total macroscopic score compared to the control (p < 0.001) and glucosamine (p < 0.05) groups. Semiquantitative histology scoring showed that both Channa and glucosamine groups had lower severity grading of total histology score compared to the control group (p < 0.001). In comparison with the control, Channa group had lower histopathological changes in three compartments of the joint compared to glucosamine group which had lower histological scoring in two compartments only. Te cartilage thickness, area, and roughness of both Channa (p < 0.05) and glucosamine (p < 0.05) groups were superior compared to the control group. However, the Channa group demonstrated signifcantly less cartilage roughness compared to the glucosamine group (p < 0.05). Serum COMP levels were lower in both Channa (p < 0.05) and glucosamine (p < 0.05) groups compared to the control group. Conclusion. Both oral administration of Channa extract and glucosamine exhibited chondroprotective action on an ACLT OA-induced rabbit model. However, Channa was superior to glucosamine in maintaining the structure of the cartilage. Hindawi Publishers 2019 Article PeerReviewed application/pdf en http://irep.iium.edu.my/87628/1/Dr.Azidah%20paper%202019.pdf Abdul Kadir, Azidah and Abdul Kadir, Arifah and Abd Hamid, Roslida and Mat Jais, Abdul Manan and Omar, Julia and Sadagatullah, Abdul Nawfar and Badrin, Salziyan and Win, Thin Thin and Kuttulebbai Naina Mohamed Salam, Sirajudeen and Salleh, Annas (2019) Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model. BioMed Research International, 2019. pp. 1-11. ISSN 2314-6133 http://doi.org/10.1155/2019/6979585 doi:10.1155/2019/6979585
institution	Universiti Islam Antarabangsa Malaysia
building	IIUM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	International Islamic University Malaysia
content_source	IIUM Repository (IREP)
url_provider	http://irep.iium.edu.my/
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Abdul Kadir, Azidah Abdul Kadir, Arifah Abd Hamid, Roslida Mat Jais, Abdul Manan Omar, Julia Sadagatullah, Abdul Nawfar Badrin, Salziyan Win, Thin Thin Kuttulebbai Naina Mohamed Salam, Sirajudeen Salleh, Annas Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model
description	Objectives. Te objective of the study is to evaluate the chondroprotective activity of Channa striatus (Channa) and glucosamine sulphate (glucosamine) on histomorphometric examinations, serum biomarker, and infammatory mediators in experimental osteoarthritis (OA) rabbit model. Design. Anterior cruciate ligament transection (ACLT) was performed to induce OA in thirtythree male New Zealand white rabbits and were randomly divided into three groups: Channa, glucosamine, and control group. Te control group received drinking water and the Channa and glucosamine groups were orally administered with 51.4 mg/kg of Channa extract and 77.5 mg/kg of glucosamine sulphate in drinking water, respectively, for eight weeks and then sacrifced. Te articular cartilage was evaluated macroscopically and histologically using semiquantitative and quantitative methods. Serum cartilage oligomeric matric protein (COMP), cyclooxygenase 2 (COX-2) enzyme, and prostaglandin E2 (PGE2) were also determined. Results. Macroscopic analysis revealed that Channa group have a signifcantly lower severity grade of total macroscopic score compared to the control (p < 0.001) and glucosamine (p < 0.05) groups. Semiquantitative histology scoring showed that both Channa and glucosamine groups had lower severity grading of total histology score compared to the control group (p < 0.001). In comparison with the control, Channa group had lower histopathological changes in three compartments of the joint compared to glucosamine group which had lower histological scoring in two compartments only. Te cartilage thickness, area, and roughness of both Channa (p < 0.05) and glucosamine (p < 0.05) groups were superior compared to the control group. However, the Channa group demonstrated signifcantly less cartilage roughness compared to the glucosamine group (p < 0.05). Serum COMP levels were lower in both Channa (p < 0.05) and glucosamine (p < 0.05) groups compared to the control group. Conclusion. Both oral administration of Channa extract and glucosamine exhibited chondroprotective action on an ACLT OA-induced rabbit model. However, Channa was superior to glucosamine in maintaining the structure of the cartilage.
format	Article
author	Abdul Kadir, Azidah Abdul Kadir, Arifah Abd Hamid, Roslida Mat Jais, Abdul Manan Omar, Julia Sadagatullah, Abdul Nawfar Badrin, Salziyan Win, Thin Thin Kuttulebbai Naina Mohamed Salam, Sirajudeen Salleh, Annas
author_facet	Abdul Kadir, Azidah Abdul Kadir, Arifah Abd Hamid, Roslida Mat Jais, Abdul Manan Omar, Julia Sadagatullah, Abdul Nawfar Badrin, Salziyan Win, Thin Thin Kuttulebbai Naina Mohamed Salam, Sirajudeen Salleh, Annas
author_sort	Abdul Kadir, Azidah
title	Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model
title_short	Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model
title_full	Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model
title_fullStr	Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model
title_full_unstemmed	Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model
title_sort	evaluation of chondroprotective activity of channa striatus in rabbit osteoarthritis model
publisher	Hindawi Publishers
publishDate	2019
url	http://irep.iium.edu.my/87628/1/Dr.Azidah%20paper%202019.pdf http://irep.iium.edu.my/87628/ http://doi.org/10.1155/2019/6979585
_version_	1688646781531848704
score	13.211869

Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model

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