Evaluation of chondroprotective activity of Channa striatus in rabbit osteoarthritis model
Objectives. Te objective of the study is to evaluate the chondroprotective activity of Channa striatus (Channa) and glucosamine sulphate (glucosamine) on histomorphometric examinations, serum biomarker, and infammatory mediators in experimental osteoarthritis (OA) rabbit model. Design. Anterior cruc...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishers
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/87628/1/Dr.Azidah%20paper%202019.pdf http://irep.iium.edu.my/87628/ http://doi.org/10.1155/2019/6979585 |
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| Summary: | Objectives. Te objective of the study is to evaluate the chondroprotective activity of Channa striatus (Channa) and glucosamine sulphate (glucosamine) on histomorphometric examinations, serum biomarker, and infammatory mediators in experimental osteoarthritis (OA) rabbit model. Design. Anterior cruciate ligament transection (ACLT) was performed to induce OA in thirtythree male New Zealand white rabbits and were randomly divided into three groups: Channa, glucosamine, and control group. Te control group received drinking water and the Channa and glucosamine groups were orally administered with 51.4 mg/kg of Channa
extract and 77.5 mg/kg of glucosamine sulphate in drinking water, respectively, for eight weeks and then sacrifced. Te articular cartilage was evaluated macroscopically and histologically using semiquantitative and quantitative methods. Serum cartilage oligomeric matric protein (COMP), cyclooxygenase 2 (COX-2) enzyme, and prostaglandin E2 (PGE2) were also determined.
Results. Macroscopic analysis revealed that Channa group have a signifcantly lower severity grade of total macroscopic score compared to the control (p < 0.001) and glucosamine (p < 0.05) groups. Semiquantitative histology scoring showed that both Channa and glucosamine groups had lower severity grading of total histology score compared to the control group (p < 0.001). In comparison with the control, Channa group had lower histopathological changes in three compartments of the joint compared to glucosamine group which had lower histological scoring in two compartments only. Te cartilage thickness, area, and roughness of both Channa (p < 0.05) and glucosamine (p < 0.05) groups were superior compared to the control group. However, the Channa group demonstrated signifcantly less cartilage roughness compared to the glucosamine group (p < 0.05). Serum COMP levels were lower in both Channa (p < 0.05) and glucosamine (p < 0.05) groups compared to the control group. Conclusion. Both oral administration of Channa extract and glucosamine exhibited chondroprotective action on an ACLT OA-induced rabbit model. However, Channa was superior to glucosamine in maintaining the structure of the cartilage. |
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