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Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry

Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose l...

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Main Authors: Ramli, Nurul Izzati, Abd-Wahab, Firdaus, Wan Salim, Wan Wardatul Amani
Format: Conference or Workshop Item
Language:English
English
Published: AIP 2018
Subjects:
TP155 Chemical engineering
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf
http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf
http://irep.iium.edu.my/68443/
https://aip.scitation.org/doi/10.1063/1.5066993
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spelling my.iium.irep.684432018-12-17T06:18:37Z http://irep.iium.edu.my/68443/ Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry Ramli, Nurul Izzati Abd-Wahab, Firdaus Wan Salim, Wan Wardatul Amani TP155 Chemical engineering TP248.13 Biotechnology Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose levels in saliva instead of in blood, by incorporating rGO-PEDOT:PSS composites as the transducer layer on a working electrode – a screen-printed carbon electrode (SPCE) 2 mm in diameter. To evaluate the performance of the biosensor, the electrochemical technique of linear sweep voltammetry (LSV) was used. The results showed sensitivity of rGO-PEDOT:PSS-GOx/SPCE in artificial saliva: 105 μA/mM (σ = 12.52) at no glucose to 0.2 mM glucose concentrations and 35.33 μA/mM (σ = 5.79) at glucose concentrations of 0.2 mM to 0.6 mM, with correlation coefficient of 0.99. The sensitivity of the biosensor in 10 mM PBS (pH 6.8) is 9.88 μA/mM (σ = 2.74), while in 100 mM potassium ferricyanide it is 10.48 μA/mM (σ = 0.49). The high sensitivity obtained in artificial saliva suggests that the rGO-PEDOT:PSS composite is suitable for use as a transducer layer for non-invasive glucose monitoring. AIP 2018-11-29 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf application/pdf en http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf Ramli, Nurul Izzati and Abd-Wahab, Firdaus and Wan Salim, Wan Wardatul Amani (2018) Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry. In: 3rd International Sciences, Technology & Engineering Conference (ISTEC) 2018 - Material Chemistry, 18th April 2018, Penang, Malaysia. https://aip.scitation.org/doi/10.1063/1.5066993 10.1063/1.5066993
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic TP155 Chemical engineering
TP248.13 Biotechnology
spellingShingle TP155 Chemical engineering
TP248.13 Biotechnology
Ramli, Nurul Izzati
Abd-Wahab, Firdaus
Wan Salim, Wan Wardatul Amani
Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
description Existing practice of glucose-level monitoring for diabetic patients involves frequent finger pricking for blood samples. This is not only constantly painful, but could make patients susceptible to infections. A non-invasive, portable, graphene-based biosensor is being developed to measure glucose levels in saliva instead of in blood, by incorporating rGO-PEDOT:PSS composites as the transducer layer on a working electrode – a screen-printed carbon electrode (SPCE) 2 mm in diameter. To evaluate the performance of the biosensor, the electrochemical technique of linear sweep voltammetry (LSV) was used. The results showed sensitivity of rGO-PEDOT:PSS-GOx/SPCE in artificial saliva: 105 μA/mM (σ = 12.52) at no glucose to 0.2 mM glucose concentrations and 35.33 μA/mM (σ = 5.79) at glucose concentrations of 0.2 mM to 0.6 mM, with correlation coefficient of 0.99. The sensitivity of the biosensor in 10 mM PBS (pH 6.8) is 9.88 μA/mM (σ = 2.74), while in 100 mM potassium ferricyanide it is 10.48 μA/mM (σ = 0.49). The high sensitivity obtained in artificial saliva suggests that the rGO-PEDOT:PSS composite is suitable for use as a transducer layer for non-invasive glucose monitoring.
format Conference or Workshop Item
author Ramli, Nurul Izzati
Abd-Wahab, Firdaus
Wan Salim, Wan Wardatul Amani
author_facet Ramli, Nurul Izzati
Abd-Wahab, Firdaus
Wan Salim, Wan Wardatul Amani
author_sort Ramli, Nurul Izzati
title Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_short Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_full Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_fullStr Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_full_unstemmed Characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
title_sort characterization of enzymatic glucose biosensor in buffer solution, in artificial saliva, and in potassium ferricyanide by linear sweep voltammetry
publisher AIP
publishDate 2018
url http://irep.iium.edu.my/68443/1/ISTEC%202018%20AIP%20Conference%20Proceeding%20Nurul%20Izzati%20et%20al%202018.pdf
http://irep.iium.edu.my/68443/7/68443%20Characterization%20of%20Enzymatic%20Glucose%20Biosensor%20in%20Buffer.pdf
http://irep.iium.edu.my/68443/
https://aip.scitation.org/doi/10.1063/1.5066993
_version_ 1643618603351670784
score 13.211869

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