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Proteomics of broiler’s liver

In this study, the differences in prote in expression from chicken liver between commercialbroilers (CB) and indigenous chicken (IC) have been analysed by using two-dimensional gel electrophoresis (2D-PAGE). The study was conducted adjacent to consumer experience in order...

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Main Authors: Abu Hassan, Muhamad Shafiq, Saidin, Muhammad Syazwan, Hamdan, Nurul Ashima, Abd Hamid, Shafida, Muhamad Bunnori, Noraslinda
Format: Article
Language:English
Published: Institute of Research and Journals 2018
Subjects:
Q Science (General)
Online Access:http://irep.iium.edu.my/64630/1/IJASEAT_MUHAMAD%20SHAFIQ.pdf
http://irep.iium.edu.my/64630/
http://www.iraj.in/journal/journal_file/journal_pdf/6-462-15302652828-17.pdf
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spelling my.iium.irep.64630 http://irep.iium.edu.my/64630/ Proteomics of broiler’s liver Abu Hassan, Muhamad Shafiq Saidin, Muhammad Syazwan Hamdan, Nurul Ashima Abd Hamid, Shafida Muhamad Bunnori, Noraslinda Q Science (General) In this study, the differences in prote in expression from chicken liver between commercialbroilers (CB) and indigenous chicken (IC) have been analysed by using two-dimensional gel electrophoresis (2D-PAGE). The study was conducted adjacent to consumer experience in order to find out the differences of the protein profile in liver tissues between the two popular types of chickens that have been offered to the Malaysians consumer for over 50 years. In 2D-PAGE, six spots were successfully identified differentially expressed in term of intensity. Those proteins are phosphoenolpyruvate carboxykinase (PEPCK) (2 spots), delta-1-pyrolline-5-carboxylate (P5CDH), soluble epoxide hydrolase (sEH), heat shock protein 90 (HSP90) and γ-actin. PEPCK and P5CDH enzymes were expressed higher in CB compared to IC, suggesting the utilization of alternative metabolic pathways for broilers’ energy metabolism. The sEH expression was found higher in CB compared to IC probably due to the process of xenobiotic metabolic activity to eliminate harmful epoxide inside the CB’s liver. HSP90 was found less in CB compared to IC, which may indicate the lack of regulation of heat stress response in CB. The higher expression of actin in IC liver suggested a good form of structure integrity in IC liver compared to CB, which may happen due to the age differences. Most of the differentially expressed proteins are involved in energy metabolism, stress regulation, and cellular structure. These differences could arise from many variables involved which may include breeds, age, feed regimen,drug treatment and rearing environment. Institute of Research and Journals 2018-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64630/1/IJASEAT_MUHAMAD%20SHAFIQ.pdf Abu Hassan, Muhamad Shafiq and Saidin, Muhammad Syazwan and Hamdan, Nurul Ashima and Abd Hamid, Shafida and Muhamad Bunnori, Noraslinda (2018) Proteomics of broiler’s liver. International Journal of Advances in Science Engineering and Technology, 6 (2). pp. 8-17. ISSN 2321-8991 http://www.iraj.in/journal/journal_file/journal_pdf/6-462-15302652828-17.pdf IJASEAT-IRAJ-DOI-11994
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Abu Hassan, Muhamad Shafiq
Saidin, Muhammad Syazwan
Hamdan, Nurul Ashima
Abd Hamid, Shafida
Muhamad Bunnori, Noraslinda
Proteomics of broiler’s liver
description In this study, the differences in prote in expression from chicken liver between commercialbroilers (CB) and indigenous chicken (IC) have been analysed by using two-dimensional gel electrophoresis (2D-PAGE). The study was conducted adjacent to consumer experience in order to find out the differences of the protein profile in liver tissues between the two popular types of chickens that have been offered to the Malaysians consumer for over 50 years. In 2D-PAGE, six spots were successfully identified differentially expressed in term of intensity. Those proteins are phosphoenolpyruvate carboxykinase (PEPCK) (2 spots), delta-1-pyrolline-5-carboxylate (P5CDH), soluble epoxide hydrolase (sEH), heat shock protein 90 (HSP90) and γ-actin. PEPCK and P5CDH enzymes were expressed higher in CB compared to IC, suggesting the utilization of alternative metabolic pathways for broilers’ energy metabolism. The sEH expression was found higher in CB compared to IC probably due to the process of xenobiotic metabolic activity to eliminate harmful epoxide inside the CB’s liver. HSP90 was found less in CB compared to IC, which may indicate the lack of regulation of heat stress response in CB. The higher expression of actin in IC liver suggested a good form of structure integrity in IC liver compared to CB, which may happen due to the age differences. Most of the differentially expressed proteins are involved in energy metabolism, stress regulation, and cellular structure. These differences could arise from many variables involved which may include breeds, age, feed regimen,drug treatment and rearing environment.
format Article
author Abu Hassan, Muhamad Shafiq
Saidin, Muhammad Syazwan
Hamdan, Nurul Ashima
Abd Hamid, Shafida
Muhamad Bunnori, Noraslinda
author_facet Abu Hassan, Muhamad Shafiq
Saidin, Muhammad Syazwan
Hamdan, Nurul Ashima
Abd Hamid, Shafida
Muhamad Bunnori, Noraslinda
author_sort Abu Hassan, Muhamad Shafiq
title Proteomics of broiler’s liver
title_short Proteomics of broiler’s liver
title_full Proteomics of broiler’s liver
title_fullStr Proteomics of broiler’s liver
title_full_unstemmed Proteomics of broiler’s liver
title_sort proteomics of broiler’s liver
publisher Institute of Research and Journals
publishDate 2018
url http://irep.iium.edu.my/64630/1/IJASEAT_MUHAMAD%20SHAFIQ.pdf
http://irep.iium.edu.my/64630/
http://www.iraj.in/journal/journal_file/journal_pdf/6-462-15302652828-17.pdf
_version_ 1643617269851357184
score 13.211869

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